[1]李君朝,申志国,纪朋曼,等.急性脑梗死患者血清FGL2 和TSPO 表达水平及其与神经功能缺损程度和预后的相关性研究[J].现代检验医学杂志,2023,38(03):138-142.[doi:10.3969/j.issn.1671-7414.2023.03.025]
　LI Jun-chao,SHEN Zhi-guo,JI Peng-man,et al.Expression Levels of Serum FGL2 and TSPO in Patients with Acute Cerebral Infarction and Their Correlation with the Degree of Neurological Impairment and Prognosis[J].Journal of Modern Laboratory Medicine,2023,38(03):138-142.[doi:10.3969/j.issn.1671-7414.2023.03.025]
点击复制

急性脑梗死患者血清FGL2 和TSPO 表达水平及其与神经功能缺损程度和预后的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年03期
页码:
138-142
栏目:
论著
出版日期:
2023-05-15

文章信息/Info

Title:
Expression Levels of Serum FGL2 and TSPO in Patients with Acute Cerebral Infarction and Their Correlation with the Degree of Neurological Impairment and Prognosis
文章编号:
1671-7414（2023）03-138-05
作者:
李君朝1; 申志国2; 纪朋曼3; 齐赛卿2; 张亚伟2
（1. 邯郸市邯钢医院神经内科，河北邯郸 056001；2. 华北医疗健康集团峰峰总医院临床药学部，河北邯郸056200；3. 河北省第七人民医院，河北保定 073000）
Author(s):
LI Jun-chao1;  SHEN Zhi-guo2;  JI Peng-man3;  QI Sai-qing2;  ZHANG Ya-wei2
（1. Department of Neurology, Handan Hangang Hospital, Hebei Handan 056001, China; 2. Department of Clinical Pharmacology, Fengfeng General Hospital, North China Medical & Health Group, Hebei Handan 056200, China; 3. the Seventh People’s Hospital of Hebei
关键词:
急性脑梗死; 纤维蛋白原样蛋白2; 转运蛋白; 神经功能缺损程度
分类号:
R743.33；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.03.025
文献标志码:
A
摘要:
目的 检测急性脑梗死（acute cerebral infarct, ACI）患者血清纤维蛋白原样蛋白2（FGL2）和转运蛋白（TSPO）水平，探讨二者与患者神经功能缺损程度及预后的关系。方法 选取2020 年8 月～ 2021 年8 月河北省邯郸市邯钢医院收治的110 例急性脑梗死患者为研究对象，另取同期体检健康者90 例为对照组。酶联免疫吸附法检测血清FGL2 和TSPO 水平；美国国立卫生研究院卒中量表（NIHSS）评估患者神经功能缺损程度，采用Spearman 等级相关分析急性脑梗死患者神经功能缺损程度与血清FGL2 和TSPO 水平的相关性；根据急性脑梗死患者预后情况将其分为预后良好组（n=76）和预后不良组（n=34），受试者工作特征（ROC）曲线分析血清FGL2 和TSPO 对急性脑梗死患者预后的预测效能。结果 与对照组比较，急性脑梗死患者血清FGL2 水平（231.52±41.29 ng/ml vs 125.26±24.16 ng/ml）和TSPO水平（6.28±1.05 ng/L vs 3.54±0.74 ng/L）升高，差异均有统计学意义（t=21.573，20.872，均P ＜ 0.05）。与轻度组比较，中度组血清FGL2（236.63±42.49ng/ml vs196.62±36.61ng/ml），TSPO（6.36±1.04ng/L vs 4.84±0.87ng/L）水平升高，与中度组比较，重度组血清FGL2（275.20±50.25ng/ml vs 236.63±42.49ng/ml），TSPO（8.26±1.32ng/L vs 6.36±1.04ng/L）水平升高，差异均有统计学意义（t=4.549，7.153；3.432，6.640，均P ＜ 0.05）。Spearman 相关性分析显示血清FGL2，TSPO 水平与神经功能缺损程度呈正相关（r=0.514，0.495，均P ＜ 0.05）。与预后良好组比较，预后不良组血清FGL2 水平（287.75±43.81 ng/ml vs 206.36±40.16 ng/ml）和TSPO 水平（7.83±1.80 ng/L vs 5.58±0.72 ng/L）升高，差异均有统计学意义（t= 9.549，9.386，均P＜ 0.05）。血清FGL2，TSPO预测急性脑梗死患者预后不良的曲线下面积（AUC）分别为0.926，0.825，联合预测的AUC 为0.975，优于各指标单独预测（Z=2.621，3.100，均P ＜ 0.05）。结论 急性脑梗死患者血清FGL2 和TSPO 水平增高，与神经功能缺损程度呈正相关，对患者的预后状况具有较高的预测效能，为临床的合理干预和改善患者预后提供依据。
Abstract:
Objective To detect the levels of serum fibrinogen-like protein 2 (FGL2) and transport protein (TSPO) in patients with acute cerebral infarction（ACI） and explore their relationship with the degree of neurological deficit and prognosis. Methods A total of 110 patients with ACI admitted to Handan Hangang Hospital, Hebei Province from August 2020 to August 2021 were regarded as the research objects, another 90 healthy subjects who underwent physical examination were regarded as the control group. Serum FGL2 and TSPO levels were measured by enzyme-linked immunosorbent assay, the National Institutes of Health Stroke Scale (NIHSS) was performed to assess the degree of neurological deficit in patients, Spearman rank correlation was used to analyze the correlation between the degree of neurological deficit and serum FGL2 and TSPO levels in patients with ACI，according to the prognosis of patients with acute cerebral infarction, they were grouped into a good prognosis group (n=76) and a poor prognosis group (n=34), receiver operating characteristic (ROC) curve was performed to analyze the predictive efficacy of serum FGL2 and TSPO on the prognosis of patients with ACI. Results Compared with the control group, the levels of serum FGL2 (231.52 ± 41.29 ng/ml vs 125.26 ± 24.16 ng/ml) and TSPO (6.28 ± 1.05 ng/L vs 3.54 ± 0.74 ng/L) in patients with ACI were higher, the differences was statistically significant (t=21.573, 20.872, all P<0.05). Compared with mild group, serum FGL2 (236.63 ± 42.49 ng/ml vs 196.62 ± 36.61 ng/ml) and TSPO (6.36 ± 1.04 ng/L vs 4.84 ± 0.87 ng/L) levels in moderate group were increased，compared with moderate group, serum FGL2 (275.20 ± 50.25 ng/ml vs 236.63 ± 42.49 ng/ ml) and TSPO (8.26 ± 1.32 ng/L vs 6.36 ± 1.04 ng/L) levels in severe group were increased，the differences were statistically significant (t=4.549，7.153，3.432, 6.640, all P<0.05)，respectively. Spearman correlation analysis showed that serum FGL2 and TSPO levels were positively correlated with the degree of neurological deficit (r=0.514, 0.495, all P<0.05). Compared with the group with good prognosis, the serum FGL2 level (287.75 ± 43.81 ng/ml vs 206.36 ± 40.16 ng/ml) and TSPO level (7.83 ± 1.80 ng/L vs 5.58 ± 0.72 ng/L) in the group with poor prognosis were higher, with difference were statistically significant (t=9.549，9.386，all P<0.05). The area under the curve (AUC) of serum FGL2 and TSPO combined to predict poor prognosis in patients with ACI was 0.926 and 0.825, respectively, and the AUC of combined prediction was 0.975, which was better than the single prediction of each index (Z=2.621, 3.100, all P<0.05). Conclusion Serum FGL2 and TSPO levels in patients with ACI were increased, which were positively correlated with the degree of neurological deficit. It have a high predictive effect on the prognosis of patients, providing a basis for reasonable clinical intervention and improvement of prognosis.

参考文献/References:

[1] 孙原, 崔凡凡, 李冬梅, 等. 急性脑梗死患者ox- LDL, hs-CRP, Hcy, FIB 表达水平及其与颈动脉狭窄 的关系[J]. 中国老年学杂志, 2020, 40(2): 250-254. SUN Yuan, CUI Fanfan, LI Dongmei, et al. Expressions of ox-LDL, hs-CRP, Hcy, FIB of acute cerebral infarction and the relationships with carotid artery stenosis [J]. Chinese Journal of Gerontology, 2020,40(2): 250-254.
[2] 代玲玲, 徐榛敏, 梁晓, 等. 近三年针刺治疗急性缺 血性脑卒中随机对照试验中结局指标现状分析[J]. 中国中药杂志, 2021, 46(12): 2949-2962. DAI Lingling, XU Zhenmin, LIANG Xiao, et al. Analysis of status quo of outcome indicators of randomized controlled trials in treatment of acute ischemic stroke with acupuncture in recent three years [J]. China Journal of Chinese Materia Medica, 2021,46(12): 2949-2962.
[3] 梁菊萍, 杨旸, 董继存. 急性脑梗死患者流行病学 调查及危险因素[J]. 中国老年学杂志, 2021, 41(12): 2484-2487. LIANG Juping, YANG Yang, DONG Jicun. Epidemiological investigation and risk factors of patients with acute cerebral infarction[J]. Chinese Journal of Gerontology, 2021, 41(12): 2484-2487.
[4] BAO Lei, ZHANG Shenyang, GONG Xiangyao, et al. Trousseau syndrome related cerebral infarction: clinical manifestations, laboratory findings and radiological features[J]. Journal of Stroke and Cerebrovascular Diseases, 2020, 29(9): 104891.
[5] SHALEV I, SELZNER N, HELMY A, et al. The role of FGL2 in the pathogenesis and treatment of hepatitis C virus infection[J]. Rambam Maimonides Medical Journal, 2010, 1(1): e0004.
[6] PATEL R, TRAYLOR J I, LATHA K, et al. Fibrinogen- like protein 2: a potential molecular target for glioblastoma treatment[J]. Expert Opinion on Therapeutic Targets, 2019, 23(8): 647-649.
[7] MELNYK M C, SHALEV I, ZHANG J, et al. The prothrombinase activity of FGL2 contributes to the pathogenesis of experimental arthritis[J]. Scandinavian Journal of Rheumatology, 2011, 40(4): 269-278.
[8] TANG Ming, CAO Xu, LI Peng, et al. Increased expression of fibrinogen-like protein 2 is associated with poor prognosis in patients with clear cell renal cell carcinoma[J]. Scientific Reports, 2017, 7(1): 12676.
[9] YUAN Bangqing, FU Fenlan, HUANG Shaokuan,et al. C5a/C5aR pathway plays a vital role in brain inflammatory injury via initiating Fgl-2 in intracerebral hemorrhage[J]. Molecular Microbiology, 2017, 54(8): 6187-6197.
[10] LI Fei, LIU Jian, GARAVITO R M, et al. Evolving understanding of translocator protein 18 kDa (TSPO) [J]. Pharmacological Research, 2015, 99: 404-409.
[11] VENNETI S, LOPRESTI B J, WILEY C A. The peripheral benzodiazepine receptor (Translocator protein 18kDa) in microglia: from pathology to imaging[J]. Progress in Neurobiology, 2006, 80(6): 308-322.
[12] 钟迪, 张舒婷, 吴波. 《中国急性缺血性脑卒中诊治 指南2018》解读[J]. 中国现代神经疾病杂志, 2019,19(11): 897-901. ZHONG Di, ZHANG Shuting, WU Bo. Interpretation of Chinese guidelines for diagnosis and treatment of acute ischemic stroke 2018[J]. Chinese Journal of Contemporary Neurology and Neurosurgery, 2019,19(11): 897-901.
[13] JEYASEELAN R D, VARGO M M, CHAE J. National institutes of health stroke scale (NIHSS) as an early predictor of poststroke dysphagia[J]. PM & R, 2015,7(6): 593-598.
[14] 袁俊亮, 张晓丹, 李海英, 等. smRSq 量表在部分中 国脑卒中人群中信度和效度的初步研究[J]. 临床神 经病学杂志, 2016, 29(3): 161-163. YUAN Junliang, ZHANG Xiaodan, LI Haiying, et al. The preliminary study of reliability and validity of simplified modified Rankin scale used in some of Chinese stroke patients [J]. Journal of Clinical Neurology, 2016, 29(3): 161-163.
[15] 陈敏, 陈秀贞, 张辉, 等. 南平市延平区2016 年急 性心脑血管病的流行病学特征[J]. 海峡预防医学杂 志, 2018, 24(3): 64-67. CHEN Min, CHEN Xiuzhen, ZHANG Hui,et al. Epidemiological characteristics of acute cardiovascular and cerebrovascular diseases in Yanping District,Nanping City in 2016[J]. Strait Journal of Preventive Medicine, 2018, 24(3): 64-67.
[16] 罗星星, 郑雪莲, 张瑞娴, 等. 高血压并发动脉粥样 硬化患者血清同型半胱氨酸水平与凝血功能的相关 性研究[J]. 现代检验医学杂志, 2021, 36(3): 144-147,150. LUO Xingxing, ZHENG Xuelian, ZHANG Ruixian,et al. Correlation between serum homocysteine level and coagulation function in patients with hypertension complicated with atherosclerosis [J]. Journal of Modern Laboratory Medicine, 2021, 36(3): 144-147, 150.
[17] 何贤禄, 王鹏宇, 何孟珈, 等. NLRP12 对小鼠病毒 性肝炎的保护作用及机制探讨[J]. 现代预防医学,2022, 49(12): 2247-2253, 2286. HE Xianlu, WANG Pengyu, HE Mengjia, et al. Exploring the protective effect and mechanism of NLRP12 against viral hepatitis in mice [J]. Modern Preventive Medicine, 2022, 49(12): 2247-2253, 2286.
[18] 王小东. 过表达sFgl2 的MSCs 调节巨噬细胞抑制 小鼠心脏移植急性排斥反应研究[D]. 天津: 天津医 科大学, 2019. WANG Xiaodong. MSCs overexpressing sFgl2 inhibit acute rejection of heart transplantation in mice by regulating macrophages [D]. Tianjin: Tianjin Medical University, 2019.
[19] 余秋实. Fgl2 和TNF-α 在急性心肌梗死患者和缺 血再灌注大鼠外周血中表达和意义[D]. 武汉: 华中 科技大学, 2010. YU Qiushi. The expression and significance of Fgl2 prothrombinase and Cytokine TNF- α in the peripheral blood of AMI patients and heart ischemiareperfusion rats models[D]. Wuhan: Huazhong University of Science and Technology, 2010.
[20] 熊武芳, 刘友迎, 黄雪梅, 等. 自身免疫性肝炎患者 外周血PBMC 中FGL2 表达及其与外周血Th17、 Treg 细胞及相关因子的关系[J]. 标记免疫分析与临 床, 2019, 26(12): 2109-2114. XIONG Wufang, LIU Youying, HUANG Xuemei, et al. The expression of FGL2 in peripheral blood of patients with autoimmune hepatitis and its relationships with Th17 and Treg cells [J]. Labeled Immunoassays and Clinical Medicine, 2019, 26(12): 2109-2114.
[21] XU Wenbin, HU Qianhua, WU Channi, et al. Serum soluble fibrinogen-like protein 2 concentration predicts delirium after acute pancreatitis[J]. Brain and Behavior,2019, 9(4): e01261.
[22] 仉慧颖, 耿威, 陈岩, 等. Fgl2 基因对H9C2 心肌 细胞凋亡的影响[J]. 中国老年学杂志, 2018, 38(3): 687-689. ZHANG Huiying, GENG Wei, CHEN Yan, et al. Effect of Fgl2 gene on apoptosis of H9C2 cardiomyocytes[J]. Chinese Journal of Gerontology, 2018, 38(3): 687-689.
[23] 陈欣, 朱力. 血清转运蛋白联合格拉斯哥评分与休 克指数比值对重型颅脑外伤患者预后的预测价值 [J]. 中华医学杂志, 2021, 101(3): 218-223. CHEN Xin, ZHU Li. Prognostic factors of severe traumatic brain injury and the predictive value of serum translocator protein combined with the reverse shock index multiplied by Glasgow Coma Scale [J]. National Medical Journal of China, 2021, 101(3): 218-223.
[24] V?LLEZ GARCIA D, DE VRIES E F J, TOYOHARA J, et al. Evaluation of [(11)C]CB184 for imaging and quantification of TSPO overexpression in a rat model of herpes encephalitis[J]. European Journal of Nuclear Medicine and Molecular Imaging, 2015, 42(7): 1106- 1118.
[25] 戴恩云. 急性缺血性脑卒中患者血清Galectin-3,TSPO 水平及意义[J]. 黑龙江医药科学, 2022, 45(2): 42-45. DAI Enyun. Serum levels of Galectin-3 and TSPO in patients with acute ischemic stroke and their significance [J]. Heilongjiang Medicine and Pharmacy,2022, 45(2): 42-45.
[26] 郭蔚虹, 孙琼其, 陈瑞娟. 创伤性脑损伤患者血清 IL-1β, MMP-9 及TSPO 水平与疾病程度及预后评 估的价值研究[J]. 现代检验医学杂志, 2021, 36(6): 162-165. GUO Weihong, SUN Qiongqi, CHEN Ruijuan. Value of levels of serum IL-1β, MMP-9 and TSPO in evaluating the severity and prognosis of traumatic brain injury [J]. Journal of Modern Laboratory Medicine,2021, 36(6): 162-165.

相似文献/References:

[1]何凤娥,刘依霜,段春艳.ACI患者TEG各项参数与PAgT和D-二聚体之间的相关性[J].现代检验医学杂志,2017,32(01):134.[doi:10.3969/j.issn.1671-7414.2017.01.037]
　HE Feng-e,LIU Yi-shuang,DUAN Chun-yan.Correlation between TEG Parameters and PAgT and D-Dimer in Patients with ACIH[J].Journal of Modern Laboratory Medicine,2017,32(03):134.[doi:10.3969/j.issn.1671-7414.2017.01.037]
[2]王 禺,李秀娥,赵 曼,等.急性脑梗死患者的NT-proBNP和Hcy及部分凝血纤溶指标变化的临床意义[J].现代检验医学杂志,2017,32(03):89.[doi:10.3969/j.issn.1671-7414.2017.03.024]
　WANG Yu,LI Xiu-e,ZHAO Man,et al.Clinical Significance of Changes in NT-proBNP，Hcy and Partial Coagulation-Fibrinolytic Indexes with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2017,32(03):89.[doi:10.3969/j.issn.1671-7414.2017.03.024]
[3]刘学芳,霍 豆,赵 茜,等.急性脑梗死患者血清VEGF,SAA和Lp-PLA2联合检测的临床研究[J].现代检验医学杂志,2018,33(04):76.[doi:10.3969/j.issn.1671-7414.2018.04.020]
　LIU Xue-fang,HUO-Dou,ZHAO-Qian,et al.Diagnostic Value of Combined Detection of VEGF,SAA and Lp-PLA2 for Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2018,33(03):76.[doi:10.3969/j.issn.1671-7414.2018.04.020]
[4]张同庆,杨文东.急性脑梗死并发幽门螺旋杆菌感染者血脂水平及氧化应激反应的观察[J].现代检验医学杂志,2018,33(02):66.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　ZHANG Tong-qing,YANG Wen-dong.Observation of Serum Lipid Level and Oxidative Stress Response in Patients with Acute Cerebral Infarction Complicated with Helicobacter Pylori Infection[J].Journal of Modern Laboratory Medicine,2018,33(03):66.[doi:10.3969/j.issn.1671-7414.2018.02.001]
[5]刘 莉,张 军.急性脑梗死患者血清hs-CRP,TNF-α和TIMP-1水平检测在疾病预后评估中的应用价值[J].现代检验医学杂志,2018,33(05):87.[doi:10.3969/j.issn.1671-7414.2018.05.023]
　LIU Li,ZHANG Jun.Value of Serum hs-CRP,TNF-α and TIMP-1 Levels in Prognostic Evaluation of Patients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2018,33(03):87.[doi:10.3969/j.issn.1671-7414.2018.05.023]
[6]陈 霏,王 碧.血清PDGF 及SDF-1 水平与急性脑梗死患者脑侧支循环建立的相关性研究[J].现代检验医学杂志,2020,35(01):82.[doi:10.3969/j.issn.1671-7414.2020.01.022]
　CHEN Fei,WANG Bi.Study on the Correlation between Levels of MMP-9 and SDF-1 in Serumand the Establishment of Cerebral Collateral Circulation inPatients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2020,35(03):82.[doi:10.3969/j.issn.1671-7414.2020.01.022]
[7]王 淼,玄洪雷.老年急性脑梗死患者血清Pannexin1 和sTREM-1 表达水平及其与病情和预后的相关性研究[J].现代检验医学杂志,2022,37(04):123.[doi:10.3969/j.issn.1671-7414.2022.04.024]
　WANG Miao,XUAN Hong-lei.Serum Pannexin1 and sTREM-1 Expression Levels in Elderly Patients with Acute Cerebral Infarction and Their Correlation with Disease and Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(03):123.[doi:10.3969/j.issn.1671-7414.2022.04.024]
[8]杨 华a,李致文b,曹明善a,等.急性脑梗死患者血清miR-130a 水平表达与溶栓后再出血转化风险的预测模型及验证[J].现代检验医学杂志,2022,37(06):28.[doi:10.3969/j.issn.1671-7414.2022.06.006]
　YANG Huaa,LI Zhi-wenb,CAO Ming-shana,et al.Prediction Model and Validation of Serum miR-130a Expression in Patients with Acute Cerebral Infarction and the Risk of Hemorrhagic Transformation after Thrombolysis[J].Journal of Modern Laboratory Medicine,2022,37(03):28.[doi:10.3969/j.issn.1671-7414.2022.06.006]
[9]聂晓慧,薛 茜,寇文辉,等.急性脑梗死患者外周血LncRNA-NEAT1，miR-93-5p 表达水平及临床意义[J].现代检验医学杂志,2022,37(06):94.[doi:10.3969/j.issn.1671-7414.2022.06.017]
　NIE Xiao-hui,XUE Qian,KOU Wen-hui,et al.Expression Level and Clinical Significance of Peripheral Blood LncRNANEAT1 and miR-93-5p in Patients with Acute Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2022,37(03):94.[doi:10.3969/j.issn.1671-7414.2022.06.017]
[10]张 磊,赵腾跃,李 彬.急性脑梗死患者血清Beclin-1，3-MST，TSP-1 表达水平及与病情和预后的相关性研究[J].现代检验医学杂志,2022,37(06):124.[doi:10.3969/j.issn.1671-7414.2022.06.023]
　ZHANG Lei,ZHAO Teng-yue,LI Bin.Serum Beclin-1, 3-MST and TSP-1 Expression Levels in Patients with Acute Cerebral Infarction and Their Correlation with Disease Condition and Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(03):124.[doi:10.3969/j.issn.1671-7414.2022.06.023]

备注/Memo

备注/Memo:
基金项目：邯郸市科学技术研究与发展计划（22422083093ZC）：注射用丹参多酚盐酸联合依达拉奉治疗急性脑梗死疗效及安全性研究。
作者简介：李君朝（1987-），男，本科，主治医师，研究方向：神经内科，E-mail：ljc1987chao@163.com。
更新日期/Last Update: 2023-05-15