[1]黎珊珊,李 珍,谢桃梅,等.糖尿病足溃疡患者血清Hsa_circ_0080425 表达水平与疾病严重程度及预后的关系研究[J].现代检验医学杂志,2023,38(04):59-62+82.[doi:10.3969/j.issn.1671-7414.2023.04.010]
　LI Shanshan,LI Zhen,XIE Taomei,et al.Relationship between Serum Hsa_circ_0080425 Expression Level and Disease Severity and Prognosis in Diabetic Foot Ulcer Patients[J].Journal of Modern Laboratory Medicine,2023,38(04):59-62+82.[doi:10.3969/j.issn.1671-7414.2023.04.010]
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糖尿病足溃疡患者血清Hsa_circ_0080425 表达水平与疾病严重程度及预后的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年04期
页码:
59-62+82
栏目:
论著
出版日期:
2023-07-15

文章信息/Info

Title:
Relationship between Serum Hsa_circ_0080425 Expression Level and Disease Severity and Prognosis in Diabetic Foot Ulcer Patients
文章编号:
1671-7414（2023）04-059-05
作者:
黎珊珊; 李 珍; 谢桃梅; 谢雪花; 李文霞
（儋州市人民医院内分泌代谢科，海南儋州 571179）
Author(s):
LI Shanshan;  LI Zhen;  XIE Taomei;  XIE Xuehua;  LI Wenxia
（Department of Endocrine Metabolism, Danzhou People’s Hospital， Hainan Danzhou 571179，China）
关键词:
糖尿病足; 溃疡; Hsa_circ_0080425; 疾病严重程度
分类号:
R587.2；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.04.010
文献标志码:
A
摘要:
目的　探讨血清Hsa_circ_0080425 在糖尿病足溃疡（diabetes foot ulcer，DFU）患者中的表达及其与疾病严重程度和预后的关系。方法　选取2020 年1 月～ 2022 年12 月儋州市人民医院收治的110 例DFU 患者（DFU 组），根据Wagner 分级标准分为1 级(n=24)，2 ～ 3 级(n=51) 和4 ～ 5 级(n=35)；按感染严重程度分为1 ～ 2 级（无和轻度感染，n=36），3 ～ 4 级（中重度感染，n=74）。根据DFU 患者预后情况分为预后良好组(n=66) 和预后不良组(n=44)。比较各组Hsa_circ_0080425，C- 反应蛋白(CRP) 及降钙素原（PCT）水平。应用多因素Logistic 回归分析影响DFU 患者预后不良的危险因素。绘制受试者工作特征曲线（ROC）分析Hsa_circ_0080425，CRP 及PCT 预测DFU 患者预后不良的价值。采用Spearman 相关分析DFU 患者血清Hsa_circ_0080425 表达水平与感染分级和Wagner 分级的相关性。结果　预后不良组Hsa_circ_0080425（1.96±0.85），CRP（57.35±16.82 mg/L）及PCT（0.93±0.26 ng/ml）均明显高于预后良好组（0.81±0.24，28.62±8.70 mg/L，0.25±0.12 ng/ml），差异具有统计学意义（t=17.984，12.847，14.513，均P<0.001）。中重度感染DFU患者血清Hsa_circ_0080425 表达水平（1.72±0.58）明显高于无和轻度感染患者（1.06±0.30），差异有统计学意义（t=11.820，P<0.001）。DFU 患者随着Wagner 分级增加，Hsa_circ_0080425 表达水平逐渐升高(4 ～ 5级＞ 2 ～ 3 级＞ 1 级；2.28±0.94,1.57±0.53,0.68±0.05），差异有统计学意义（F=24.716，P<0.001)。多因素Logistic回归分析显示，Hsa_circ_0080425，CRP 及PCT 水平升高是影响DFU 患者预后不良的危险因素（P<0.001）。ROC 曲线显示，Hsa_circ_0080425 ≥ 1.60 预测DFU 患者预后不良的AUC 最大（0.879，95%CI：0.816 ～ 0.940）。相关分析显示，DFU 患者血清Hsa_circ_0080425 表达水平与感染程度分级和Wagner 分级均呈正相关（r=0.706， 0.775，均P<0.001）。结论　Hsa_circ_0080425 高表达与疾病严重程度及预后不良有关，是预测DFU 患者预后不良的分子标志物。
Abstract:
Objective　To investigate the expression of serum Hsa_circ_0080425 in patients with diabetic foot ulcer (DFU) and its relationship with the severity and prognosis. Methods　A total of 110 DFU patients (DFU group) admitted to Danzhou People’s Hospital from January 2020 to December 2022 were selected and divided into grade 1 (n=24), grade 2 ～ 3 (n=51) and grade 4 ～ 5 (n=35) according to Wagner grading criteria. According to the severity of infection, 36 cases were classified into 1 ～ 2 grades (mild infection) and 74 cases were classified into 3 ～ 4 grades (moderate and severe infection). According to the prognosis of DFU patients, they were divided into 66 patients with good prognosis and 44 patients with poor prognosis. The levels of Hsa_ circ_ 0080425, C-reactive protein (CRP) and procalcitonin (PCT) were compared in each group. Multivariate logistic regression was used to analyze the risk factors of poor prognosis in patients with DFU. Draw the working characteristic curve (ROC) of subjects to analyze Hsa_circ_0080425，CRP and PCT predict the poor prognosis of patients with DFU. Spearman correlation was used to analyze the correlation between serum Hsa_circ_0080425 expression level and infection grade and Wagner grade in DFU patients. Results　The Hsa_circ_0080425（1.96±0.85），CRP（57.35±16.82 mg/L） and PCT （0.93±0.26 ng/ml）in the poor prognosis group were significantly higher than those in the good prognosis group（0.81±0.24， 28.62±8.70 mg/L，0.25±0.12 ng/ml）, and the differences were statistically significant（t=17.984，12.847，14.513，all P<0.001）. The serum Hsa_circ_0003928(1.72±0.58 vs 1.06±0.30) expression level in patients with moderate and severe DFU infection was significantly higher than that in patients with no and mild infection，the difference was statistically significant (t=11.820，P<0.001). The expression level of Hsa_circ_0080425 in DFU patients increased with the increase of Wagner grade (4 ～ 5 级＞ 2 ～ 3 级＞ 1 级；2.28±0.94,1.57±0.53,0.68±0.05）, and the difference was statistically significant（F=24.716， P<0.001). Multivariate Logistic regression analysis showed that the elevated levels of Hsa_circ_0080425, CRP and PCT were risk factors for poor prognosis in DFU patients (P<0.001). ROC curve showed that Hsa_circ_0080425 ≥ 1.60 had the greatest AUC (0.879，95%CI：0.816 ～ 0.940) in predicting poor prognosis in DFU patients. Correlation analysis showed that serum Hsa_circ_0080425 expression level of DFU patients were positively correlated with infection grade and Wagner grade (r=0.706， 0.775, all P<0.001). Conclusion　The high expression of Hsa_circ_0080425 is associated with the severity of the disease and poor prognosis, and is a molecular marker for predicting poor prognosis in DFU patients.

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备注/Memo

备注/Memo:
作者简介：黎珊珊（1989-）, 女，本科，主治医师，主要从事临床内分泌疾病研究,E-mail：lishanshan1206@126.com。
更新日期/Last Update: 2023-07-15