[1]白 雪,董巧云,赵 丽,等.帕金森病患者血清miR-7 和α-Syn 表达水平与认知功能障碍的相关性研究[J].现代检验医学杂志,2023,38(04):78-82.[doi:10.3969/j.issn.1671-7414.2023.04.014]
　BAI Xue,DONG Qiaoyun,ZHAO Li,et al.Correlation between Serum miR-7 and α-Syn Expression Levels and Cognitive Impairment in Patients with Parkinson’s Disease[J].Journal of Modern Laboratory Medicine,2023,38(04):78-82.[doi:10.3969/j.issn.1671-7414.2023.04.014]
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帕金森病患者血清miR-7 和α-Syn 表达水平与认知功能障碍的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年04期
页码:
78-82
栏目:
论著
出版日期:
2023-07-15

文章信息/Info

Title:
Correlation between Serum miR-7 and α-Syn Expression Levels and Cognitive Impairment in Patients with Parkinson’s Disease
文章编号:
1671-7414（2023）04-078-05
作者:
白 雪; 董巧云; 赵 丽; 姚 彦; 王 博
（沧州市中心医院神经内五科，河北沧州 061000）
Author(s):
BAI Xue; DONG Qiaoyun; ZHAO Li; YAO Yan; WANG Bo
（Department of Neurology, Cangzhou Central Hospital, Hebei Cangzhou 061000, China）
关键词:
帕金森病; 微小核糖核酸-7; α- 突触核蛋白; 认知功能障碍
分类号:
R742.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.04.014
文献标志码:
A
摘要:
目的　通过检测帕金森病（Parkinson's disease，PD）患者血清微小核糖核酸（miRNA，miR）-7 和α- 突触核蛋白（α-synuclein，α-Syn）水平，分析两者与认知功能障碍的关系。方法　收集2020 年1 月～ 2022 年1 月在沧州市中心医院就诊的PD 患者106 例作为PD 组，并根据Hoehn-Yahr 分级标准分为六个等级，随机选择同期性别、年龄相匹配的106 例健康体检者作为对照组。采用qRT-PCR 法检测两组血清miR-7 水平，双抗夹心酶联免疫吸附试验（enzymelinked immunosorbent assay，ELISA）法检测血清α-Syn 水平；采用简易精神状态量表（mini-mental state examination，MMSE）和蒙特利尔认知评估量表（montreal cognitive assessment，MoCA）评估PD 患者认知功能情况；采用Spearman法分析血清miR-7，α-Syn 水平与MMSE 评分、MoCA 评分的相关性，采用Pearson 法分析PD 患者血清miR-7 与α-Syn水平的相关性。结果　PD 组血清miR-7（6.95±1.74）水平低于对照组（8.21±1.35），血清α-Syn（6.28±1.05 μg/L）水平高于对照组（3.52±0.56 μg/L），差异有统计学意义（t=5.890，23.879，均P ＜ 0.05）。PD 患者Hoehn-Yahr 1 级、2 级、2.5 级、3 级、4 级和5 级血清miR-7（8.03±0.99，7.65±0.76，7.12±1.06，6.64±0.65，6.31±0.73，5.93±0.82）水平逐渐降低，α-Syn（3.79±0.39，4.29±0.81，5.48±1.07，6.37±0.82，7.55±1.34，9.01±0.76 μg/L）水平逐渐升高，差异均有统计学意义（F=13.201，55.330，均P ＜ 0.05）。Spearman 法分析显示，PD 患者血清miR-7 水平和MMSE 评分、MoCA 评分均呈正相关（r=0.574，0.597，均P ＜ 0.05）；血清α-Syn 水平和MMSE 评分、MoCA 评分均呈负相关（r=-0.550，-0.537，均P ＜ 0.05）。Pearson 法分析显示，PD 患者血清miR-7 与α-Syn 水平呈负相关（r=-0.608，P ＜ 0.05）。结论　随着PD 患者病情加重，血清miR-7 水平明显降低，α-Syn 水平明显升高，且与患者认知功能障碍密切相关。
Abstract:
Objective　To detect the levels of serum miRNA (miR) -7 and α-synuclein (α-Syn) in patients with Parkinson’s disease (PD), and analyze the relationship between the two and cognitive dysfunction. Methods　106 patients with PD in Cangzhou Central Hospital from January 2020 to January 2022 were selected as PD group, and they were divided into six grades according to Hoehn-Yahr grading standard, 106 healthy people matched with gender and age in the same period were randomly selected as the control group. The qRT-PCR method to detect serum miR-7 levels in the two groups, double resistant sandwich enzyme linked immunosorbent assay (ELISA) method to detect serum α-Syn levels, and the mini-mental state examination (MMSE) and montreal cognitive assessment (MoCA) was used to assess the cognitive function of PD patients. Spearman method was used to analyze the correlation between serum miR-7, α-Syn level and MMSE score, MoCA score, Pearson method was used to analyze the correlation between serum miR-7 and α-Syn level in PD patients. Results　The level of serum miR-7 (6.95±1.74) in the PD group was lower than that of the control group (8.21±1.35), and the level of serum α-Syn (6.28±1.05 μg/L) was higher than that of the control group (3.52±0.56 μg/L), the difference was statistically significant (t=5.890, 23.879, all P<0.05). The level of serum miR-7 (8.03±0.99，7.65±0.76，7.12±1.06，6.64±0.65，6.31±0.73，5.93±0.82) in Hoehn-Yahr grade 1， 2，2.5，3，4 and 5 patients with PD gradually decreased, and the level of α-Syn (3.79±0.39，4.29±0.81， 5.48±1.07，6.37±0.82，7.55±1.34，9.01±0.76 μg/L) gradually increased, with a statistically significant difference (F=13.201,55.330, all P<0.05). Spearman analysis showed that the serum miR-7 level in PD patients was positively correlated with MMSE score and MoCA score (r=0.574, 0.597, all P<0.05), while the serum α-Syn level was negatively correlated with MMSE score and MoCA score (r=-0.550, -0.537, all P<0.05). Pearson analysis showed that there was a negative correlation between serum miR-7 and α-Syn levels in PD patients (r=-0.608，P<0.05). Conclusion　With the aggravation of PD patients, the level of serum miR-7 was significantly decreased, and the level of α-Syn was significantly increased, which was closely related to cognitive dysfunction.

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备注/Memo

备注/Memo:
基金项目：河北省2020 年度医学科学研究课题计划（20200301）：早期帕金森病患者嗅觉与认知障碍的特点及其相关性研究。
作者简介：白雪（1986-），女，硕士，主治医师，研究方向：神经内科，E-mail：xbj381@163.com。
通讯作者：董巧云，主任医师，研究方向：神经内科，E-mail：Dqy-20051048@163.com。
更新日期/Last Update: 2023-07-15