[1]沈华,盛晓鹃.HPV 阳性宫颈癌组织中IGF2BP2 和RPRD1B 的表达水平及临床价值研究[J].现代检验医学杂志,2023,38(05):121-126.[doi:10.3969/j.issn.1671-7414.2023.05.023]
　SHEN Hua,SHENG Xiaojuan.Expression and Clinical Value of IGF2BP2 and RPRD1B in HPV Positive Cervical Cancer Tissues[J].Journal of Modern Laboratory Medicine,2023,38(05):121-126.[doi:10.3969/j.issn.1671-7414.2023.05.023]
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HPV 阳性宫颈癌组织中IGF2BP2 和RPRD1B 的表达水平及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
121-126
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Expression and Clinical Value of IGF2BP2 and RPRD1B in HPV Positive Cervical Cancer Tissues
文章编号:
1671-7414（2023）05-121-06
作者:
沈华; 盛晓鹃
（南通市海门区人民医院妇产科，江苏南通 226100）
Author(s):
SHEN Hua;  SHENG Xiaojuan
（Department of Obstetrics and Gynecology, Nantong Haimen District People’s Hospital, Jiangsu Nantong 226100, China)
关键词:
人乳头瘤病毒; 宫颈癌; 胰岛素样生长因子2 结合蛋白2; 细胞核前mRNA 结构域的调节因子1B
分类号:
R737.33；R730.43
DOI:
10.3969/j.issn.1671-7414.2023.05.023
文献标志码:
A
摘要:
目的 研究人乳头瘤病毒（human papilloma virus，HPV）阳性宫颈癌组织中胰岛素样生长因子2 结合蛋白2(insulin-like growth factor2 binding protein 2，IGF2BP2) 与细胞核前mRNA 结构域调节因子1B(regulatory nuclearpre-mRNA domain containing 1B，RPRD1B) 的表达及临床价值。方法 选取自2018 年1 月～ 2019 年1 月期间于南通市海门区人民医院诊治的82 例HPV 阳性宫颈癌患者的癌组织和癌旁组织，以41 例HPV 阴性宫颈癌组织为对照。应用免疫组织化学分析组织中IGF2BP2，RPRD1B 蛋白表达。Spearman 秩相关分析IGF2BP2 与RPRD1B 蛋白表达的相关性。Kaplan-Meier 生存分析IGF2BP2，RPRD1B 蛋白表达对生存预后的影响。COX 比例风险模型分析影响HPV 阳性宫颈癌患者生存预后的因素。结果 HPV 阳性癌组织IGF2BP2，RPRD1B 蛋白阳性率高于HPV 阴性癌组织（67.07% vs 9.76%，6.10%）及癌旁正常组织（70.73% vs 17.07%，7.32%），差异具有统计学意义（χ2=35.978，65.705；31.582，62.290，均P<0.05）。IGF2BP2 与RPRD1B 蛋白表达呈显著正相关（r=0.717，P<0.05）。肿瘤FIGO 分期Ⅱ A 期、伴淋巴结转移HPV 阳性宫颈癌组织中IGF2BP2(82.61%，90.63%)，RPRD1B(86.95%，93.75%) 蛋白阳性率分别高于Ⅰ A ～Ⅰ B期（47.22%，52.00%）、无淋巴结转移组织（50.00%，56.00%），差异均具有统计学意义（χ2=11.450 ～ 13.432，均P＜ 0.05）。IGF2BP2 阳性组三年累积生存率低于IGF2BP2 阴性组（65.45% vs 88.89%）、RPRD1B 阳性组三年累积生存率低于RPRD1B 阴性组（65.32% vs 91.67%），差异具有统计学意义（Log Rank χ2=6.487，5.192，均P<0.05）。FIGO分期Ⅱ A 期（OR=1.579，95%CI=1.042 ～ 2.393）、并发淋巴结转移（OR=1.960，95%CI=1.180 ～ 3.256），IGF2BP2阳性（OR=1.786，95%CI=1.226 ～ 2.602）和RPRD1B 阳性（OR=1.602，95%CI=1.119 ～ 2.293）是影响HPV 阳性宫颈癌患者生存预后的独立危险因素。结论 宫颈癌中IGF2BP2 和RPRD1B 表达升高，两者与FIGO 分期、淋巴结转移有关，是影响HPV 阳性宫颈癌患者预后的独立危险因素。
Abstract:
Objective To study the expression and clinical significance of insulin-like growth factor 2 binding protein 2 (IGF2BP2) and regulatory nuclear pre-mRNA domain containing 1B (RPRD1B) in HPV-positive cervical cancer tissues. Methods From January 2018 to January 2019, 82 cases of HPV-positive cervical cancer tissues and paracancerous tissues collected in Nantong Haimen District People’s Hospital were selected as the study subjects, and 41 cases of HPV-negative cervical cancer tissues were selected as the control. Immunohistochemistry was used to analyze the expression of IGF2BP2 and RPRD1B protein in tissues. Spearman rank correlation analysis was used to analyze the correlation between IGF2BP2 and RPRD1B protein expression. Kaplan-Meier survival analysis was used to analyze the effect of IGF2BP2, RPRD1B protein expression on survival prognosis in HPV-positive cervical cancer patients. COX proportional risk model was used to analyze factors affecting the survival and prognosis of HPV positive cervical cancer patients. Results The positive rate of IGF2BP2 and RPRD1B protein in HPV-positive cancer tissue was significantly higher than that in HPV-negative cancer tissue（67.07% vs 9.76%, 6.10%） and adjacent normal tissue（70.73% vs 17.07%，7.32%）, and the differences were statistically significant （χ2=35.978, 65.705; 31.582, 69.290， all P<0.05). IGF2BP2 was positively correlated with RPRD1B protein expression (r=0.717, P<0.05). The positive rates of IGF2BP2 (82.61% , 90.63%) and RPRD1B (86.95%, 93.75%) protein expression in cervical cancer tissues with FIGO stage IIA and HPV positive lymph node metastasis were higher than those in stage IA to stage IB （47.22%，52.00%）and tissues without lymph node metastasis（50.00%，56.00%），and the differences were statistically significant（χ2=11.450 ～ 13.432，all P ＜ 0.05）. The 3-year cumulative survival rates of patients in the IGF2BP2 positive expression group and RPRD1B positive expression group were lower than those in the IGF2BP2 negative expression group(65.45% vs 88.89%) and RPRD1B negative expression group(65.32% vs 91.67%), respectively (Log Rank χ2=6.487， 5.192，P<0.05）. FIGO stage Ⅱ A (OR=1.579，95% CI=1.042 ～ 2.393), combined with lymph node metastasis (OR=1.960， 95% CI=1.180 ～ 3.256), IGF2BP2 positive (OR=1.786，95% CI=1.226 ～ 2.602), RPRD1B positive (OR=1.602，95% CI=1.119 ～ 2.293) were independent risk factors affecting the survival and prognosis of HPV-positive cervical cancer patients. Conclusion The increased expression of IGF2BP2 and RPRD1B in cervical cancer were related to FIGO stage and lymph node metastasis, and both of them were independent risk factors affecting the prognosis of HPV-positive cervical cancer patients.

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备注/Memo

备注/Memo:
基金项目：江苏省科学技术厅资助项目( 编号：20180537)：高危型HPVE6/E7 蛋白在宫颈癌中的作用机制研究。
作者简介：沈华（1978-），女，本科，副主任医师，研究方向：妇产科临床，E-mail：shenhua53261@163.com。
通讯作者：盛晓鹃（1979-），女，本科，副主任医师，研究方向：妇产科临床。
更新日期/Last Update: 2023-09-15