[1]赵兴东a,刘木波b,张艳金a,等.老年膝骨性关节炎患者血清SIRT1-NT 与SIRT1-CT 水平及NT/CT 比值与疾病严重程度及其诊断价值研究[J].现代检验医学杂志,2023,38(05):138-141+194.[doi:10.3969/j.issn.1671-7414.2023.05.026]
　ZHAO Xingdonga,LIU Mubob,ZHANG Yanjina,et al.Study on the Relationship between Serum SIRT1-NT and SIRT1-CT Levels, NT/CT Ratio, Disease Severity and Diagnostic Value in Elderly Patients with Knee Osteoarthritis[J].Journal of Modern Laboratory Medicine,2023,38(05):138-141+194.[doi:10.3969/j.issn.1671-7414.2023.05.026]
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老年膝骨性关节炎患者血清SIRT1-NT 与SIRT1-CT 水平及NT/CT 比值与疾病严重程度及其诊断价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
138-141+194
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Study on the Relationship between Serum SIRT1-NT and SIRT1-CT Levels, NT/CT Ratio, Disease Severity and Diagnostic Value in Elderly Patients with Knee Osteoarthritis
文章编号:
1671-7414（2023）05-138-05
作者:
赵兴东a; 刘木波b; 张艳金a; 邬夏荣a
（贵州航天医院a. 骨科；b. 中心实验室, 贵州遵义 563000）
Author(s):
ZHAO Xingdonga; LIU Mubob; ZHANG Yanjina; WU Xiaronga
（a. Department of Orthopedics; b. Central Laboratory, Guizhou Aerospace Hospital, Guizhou Zunyi 563000，China）
关键词:
老年膝骨性关节炎; 去乙酰化酶1; N 端多肽片段; C 端多肽片段
分类号:
R684.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.05.026
文献标志码:
A
摘要:
目的 研究血清去乙酰化酶1（sirtuin1，SIRT1）N 端多肽片段（NT），去乙酰化酶1 C 端多肽片段（CT）及SIRT1 NT/CT 比值与老年膝骨性关节炎(knee osteoarthritis，KOA) 疾病严重程度及诊断价值。方法 选取2018 年6月～ 2022 年8 月期间贵州航天医院诊治的164 例老年KOA 患者作为研究对象（KOA 组），根据K-L 分级分为轻中度组（K-L 2 ～ 3 级，n=74）和重度组（K-L 4 级，n=90），以同期健康体检的健康老年人群82 例为对照组。酶联免疫吸附实验检测血清SIRT1-NT 和SIRT1-CT 水平，并计算SIRT1-NT/CT 比值。分析血清SIRT1-NT，SIRT1-CT 及NT/CT比值与老年KOA 患者疾病严重程度的关系。Spearman 秩相关分析血清SIRT1-NT，SIRT1-CT 及NT/CT 比值与K-L 分级的相关性。多因素Logistic 回归分析影响老年KOA 发生的因素。受试者工作特征曲线分析血清SIRT1-NT，SIRT1-CT 及NT/CT 比值对老年KOA 的诊断价值。结果 KOA 组血清SIRT1-NT（7.91±1.43mg/L），SIRT1-CT（2.14±0.36mg/L）及NT/CT 比值（3.77±0.48）均高于对照组（3.21±1.20mg/L，1.34±0.25mg/L，2.41±0.43），差异具有统计学意义(t=25.590，18.055，21.671，均P<0.05)。重度组KOA 患者血清SIRT1-NT（9.23±1.38mg/L），SIRT1-CT（2.30±0.33mg/L）及NT/CT 比值（4.21±0.50）高于轻中度组患者（6.30±1.50mg/L，1.95±0.41mg/L，3.23±0.44），差异具有统计学意义(t=13.009，6.057，13.178，均P<0.05)。老年KOA 患者血清SIRT1-NT，SIRT1-CT 及NT/CT 比值与K-L 分级呈显著正相关（r=0.645，0.573，0.653，均P<0.05）。血清SIRT1-NT（OR=1.594，95%CI：1.252 ～ 2.028），SIRT1-CT（OR=1.589，95%CI：1.258 ～ 2.006）及NT/CT 比值（OR=1.733，95%CI：1.249 ～ 2.404）升高是影响老年KOA 发生的独立危险因素。血清SIRT1-NT/CT 比值对诊断老年KOA 的曲线下面积（95%CI）为0.884(0.838 ～ 0.919)，大于血清SIRT1-NT0.836(0.789 ～ 0.874) 和SIRT1-CT 0.747(0.704 ～ 0.799)，差异具有统计学意义（Z=4.018，2.547，均P<0.05）。结论 老年KOA 患者血清SIRT1-NT，SIRT1-CT 及NT/CT 比值升高，三者与老年KOA 疾病严重程度有关，SIRT1-NT/CT 比值对老年KOA 具有较高的诊断价值。
Abstract:
Objective To study the severity and diagnostic value of serum N-terminal polypeptide fragment (NT), SIRT1 C-terminal polypeptide fragment (CT) and NT/CT ratio in elderly patients with knee osteoarthritis (KOA). Methods 164 elderly KOA patients treated in Guizhou Aerospace Hospital from June 2018 to August 2022 were selected as the study subjects (KOA group). According to K-L classification, they were divided into mild to moderate group (K-L 2~3, n=74) and severe group (K-L 4, n=90), and 82 healthy elderly people in the same period were selected as the control group. The serum levels of SIRT1- NT and SIRT1-CT were detected by enzyme-linked immunosorbent assay, and the ratio of SIRT1-NT/CT was calculated. The relationship between serum SIRT1-NT, SIRT1-CT and NT/CT ratio and the disease severity of elderly KOA patients was statistically analyzed. Spearman rank correlation was used to analyze the correlation between serum SIRT1-NT, SIRT1-CT and NT/CT ratio and K-L grade. Multivariate logistic regression analysis was used to analyze the factors affecting the occurrence of elderly KOA. The diagnostic value of serum SIRT1-NT, SIRT1-CT and NT/CT ratio for elderly KOA was analyzed by ROC curve. Results Serum SIRT1-NT (7.91 ± 1.43mg/L), SIRT1-CT (2.14 ± 0.36mg/L) and NT/CT ratio (3.77 ± 0.48) in KOA group were significantly higher than those in the control group（3.21±1.20mg/L，1.34±0.25mg/L，2.41±0.43），and the differences were statistically significant (t=25.590，18.055，21.671，all P<0.05). The serum SIRT1-NT (9.23 ± 1.38mg/L), SIRT1-CT (2.30 ± 0.33mg/L) and NT/CT ratio (4.21 ± 0.50) in severe KOA patients were significantly higher than those in mild to moderate KOA patients（6.30±1.50mg/L，1.95±0.41mg/L，3.23±0.44）, and the differences were statistically significant (t=13.009，6.057，13.178， all P<0.05). The serum SIRT1-NT, SIRT1-CT and NT/CT ratio in elderly KOA patients were significantly positively correlated with K-L grading (r=0.645，0.573，0.653， all P<0.05). Elevated serum SIRT1-NT (OR=1.594, 95% CI ：1.252 ～ 2.028), SIRT1-CT (OR=1.589, 95% CI：1.258 ～ 2.006) and NT/CT ratio (OR=1.733, 95% CI：1.249 ～ 2.404) were independent risk factors for the occurrence of elderly KOA. The area under the curve (95% CI) of serum SIRT1-NT/CT ratio for the diagnosis of elderly KOA was 0.884 (0.838 ～ 0.919), which was greater than serum SIRT1- NT 0.836 (0.789 ～ 0.874) and SIRT1-CT 0.747 (0.704 ～ 0.799), the differences were statistically significant (Z=4.018, 2.547, all P<0.05). Conclusion The increase of serum SIRT1-NT, SIRT1-CT and NT/CT ratio in elderly patients with KOA were related to the severity of KOA. The ratio of SIRT1-NT/CT has high diagnostic value for KOA in the elderly.

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备注/Memo

备注/Memo:
基金项目：贵州省科技支撑计划（黔科合SY 字[2020]1033 号）：不同方法诊断老年膝关节骨性关节炎的临床观察。
作者简介：赵兴东(1976-)，男，硕士研究生，主任医师，研究方向：四肢创伤修复，E-mail：zhaoxingdong59@163.com。
更新日期/Last Update: 2023-09-15