[1]祁秋霞,吴良宇,张文雅.早产儿坏死性小肠结肠炎患者血清miR-21-3p 和miR-22-3p 水平表达与病情严重程度的分析研究[J].现代检验医学杂志,2023,38(06):59-63+70.[doi:10.3969/j.issn.1671-7414.2023.06.011]
　QI Qiuxia,WU Liangyu,ZHANG Wenya.Study on the Relationship between Serum miR-21-3p and miR-22-3p Expression Levels and Severity of Necrotizing Enterocolitis in Premature Infants[J].Journal of Modern Laboratory Medicine,2023,38(06):59-63+70.[doi:10.3969/j.issn.1671-7414.2023.06.011]
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早产儿坏死性小肠结肠炎患者血清miR-21-3p 和miR-22-3p 水平表达与病情严重程度的分析研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年06期
页码:
59-63+70
栏目:
论著
出版日期:
2023-11-15

文章信息/Info

Title:
Study on the Relationship between Serum miR-21-3p and miR-22-3p Expression Levels and Severity of Necrotizing Enterocolitis in Premature Infants
文章编号:
1671-7414（2023）06-059-06
作者:
祁秋霞; 吴良宇; 张文雅
（海南省人民医院新生儿科，海口 570100）
Author(s):
QI Qiuxia;  WU Liangyu;  ZHANG Wenya
（Department of Neonatology, Hainan General Hospital, Haikou 570100, China）
关键词:
坏死性小肠结肠炎; 微小核糖核酸-21-3p; 微小核糖核酸-22-3p
分类号:
R574.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.06.011
文献标志码:
A
摘要:
目的 探究坏死性小肠结肠炎（necrotizing enterocolitis，NEC）早产儿血清微小核糖核酸（micro RNA，miR）-21-3p 和微小核糖核酸（micro RNA，miR）-22-3p 水平与病情严重程度的分析。方法 选取海南省人民医院2020 年3 月～ 2022 年12 月收治的112 例NEC 早产儿为NEC 组，根据患儿病情严重程度将其分为轻度组（n=35）、中度组（n=47）和重度组（n=30）；同时根据患儿肠道菌群紊乱程度将其分为Ⅰ级紊乱组（n=34）、Ⅱ级紊乱组（n=52）和 Ⅲ级紊乱组（n=26）。另选取在该院同期体检健康的早产儿100 例作为对照组。采用实时荧光定量PCR（qRT-PCR）测定研究对象血清miR-21-3p 和miR-22-3p 水平；采用spearman 分析血清miR-21-3p 和miR-22-3p 水平与NEC 患儿病情程度和肠道紊乱程度相关性；采用受试者工作特征（ROC）曲线评价血清miR-21-3p 和miR-22-3p 水平在预测NEC中重度患儿中的价值。结果 NEC组肿瘤坏死因子-α（TNF-α）（36.27±8.56 pg/ml）、白细胞介素-6（IL-6）（429.52±68.36pg/ml）、白细胞介素-10（IL-10）（25.83±6.46 pg/ml）和miR-21-3p（2.16±0.73 ）水平均较对照组（12.26±2.73 pg/ml，14.34±3.36 pg/ml，3.62±1.05 pg/ml ，0.92±0.28）上调，血清miR-22-3p（0.74±0.26 ）水平较对照组（1.36±0.42）下调，差异具有统计学意义（t=26.850，60.654，33.974，15.967，13.069，均P<0.05）；NEC 患儿血清miR-21-3p 水平随患儿肠道菌群紊乱程度和病情严重程度加重而逐渐升高（F=53.934，55.720，均P<0.05）；血清miR-22-3p 水平随患儿肠道菌群紊乱程度和病情严重程度加重而逐渐降低，差异具有统计学意义（F=18.037，24.017，均P<0.05）；miR-21-3p 与NEC 患儿病情严重程度以及肠道菌群紊乱程度呈正相关（r=0.516，0.531，均P<0.05），miR-22-3p 与NEC 患儿病情严重程度以及肠道菌群紊乱程度呈负相关（r=-0.504, -0.529，均P<0.05）；ROC 结果显示，血清miR-21-3p，miR-22-3p 预测NEC 患儿是否为中重度患儿的AUC 分别为0.837，0.852，两者联合预测的AUC 为0.912，均高于两者单独预测，且特异度为97.14 %。结论 NEC 早产儿血清miR-21-3p 水平上调，miR-22-3p 水平下调，且两者与肠道菌群紊乱程度和病情严重程度相关。
Abstract:
Objective To explore the relationship between serum levels of microRNA (miR) -21-3p and microRNA (miR) -22- 3p in premature infants with necrotizing enterocolitis (NEC) and the severity of the condition. Methods A total of 112 premature infants with NEC admitted to Hainan General Hospital from March 2020 to December 2022 were regarded as NEC group. According to the severity of the disease, the children were grouped into mild group (n=35), moderate group (n=47) and severe group (n=30). Meantime, according to the degree of intestinal flora disorder, the children were grouped into grade I disorder group (n=34), grade II disorder group (n=52) and grade III disorder group (n=26). In addition, 100 premature infants with healthy physical examination meantime in the hospital were included as the control group. The serum levels of miR-21-3p and miR-22-3p were measured by real-time fluorescence quantitative PCR (qRT-PCR). Spearman was applied to analyze the correlation between the levels of serum miR-21-3p and miR-22-3p and the degree of disease and intestinal disorders in children with NEC. The value of serum miR-21-3p and miR-22-3p levels in predicting moderate and severe NE children was evaluated by subject working characteristic (ROC) curve. Results Compared with the control group, the levels of tumor necrosis factor-α (TNF-α) (36.27±8.56 pg/ml), interleukin-6 (IL-6) (429.52±68.36 pg/ml), interleukin-10 (IL-10) (25.83±6.46 pg/ml) and miR-21-3p (2.16±0.73) in NEC group were increased（ 12.26±2.73 pg/ml，14.34±3.36 pg/ml，3.62±1.05 pg/ml ，0.92±0.28）, while the level of serum miR-22-3p (0.74±0.26 vs 1.36±0.42) was decreased, and the differences were statistically significant (t=26.850，60.654，33.974，15.967，13.069, all P<0.05). The level of serum miR-21-3p in children with NEC gradually increased with the degree of intestinal flora disorder and the severity of the disease (F=53.934, 55.720, all P<0.05), while the level of serum miR-22-3p gradually decreased with the degree of intestinal flora disorder and the severity of the disease (F=18.037, 24.017, all P<0.05). MiR-21-3p was positively correlated with the severity of NEC and the degree of intestinal flora disorders(r=0.516, 0.531, P<0.05), while miR-22-3p was negatively correlated with the severity of NEC and the degree of intestinal flora disorders (r=-0.504, -0.529, P<0.05). ROC results showed that the AUC predicted by serum miR-21-3p and miR- 22-3p was 0.837 and 0.852, respectively, and the AUC predicted by the combination of the two was 0.912, which was higher than that predicted by the two alone, and the specificity was 97.14%. Conclusion The serum miR-21-3p level of NEC premature infants was up-regulated, and the miR-22-3p level was down-regulated, and both were related to the degree of intestinal flora disorder and the severity of the disease.

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备注/Memo

备注/Memo:
基金项目：2020 年海南省卫生健康行业科研项目（20A200029）：kasai 术对胆道闭锁患儿免疫功能及肠道菌群的影响。
作者简介：祁秋霞（1988-），女，硕士，主治医师，研究方向：新生儿，E-mail：k6s5225e@163.com。
更新日期/Last Update: 2023-11-15