[1]陈 静,邓健美,周瑞娴,等.复发性流产患者胎盘绒毛中FTO mRNA 和ALKBH5 mRNA 水平表达及临床价值研究[J].现代检验医学杂志,2023,38(06):71-74+97.[doi:10.3969/j.issn.1671-7414.2023.06.013]
　CHEN Jing,DENG Jianmei,ZHOU Ruixian,et al.Study on the Expression and Clinical Value of FTO mRNA and ALKBH5 mRNA Levels in Placental Villi of Patients with Recurrent Miscarriage[J].Journal of Modern Laboratory Medicine,2023,38(06):71-74+97.[doi:10.3969/j.issn.1671-7414.2023.06.013]
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复发性流产患者胎盘绒毛中FTO mRNA 和ALKBH5 mRNA 水平表达及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年06期
页码:
71-74+97
栏目:
论著
出版日期:
2023-11-15

文章信息/Info

Title:
Study on the Expression and Clinical Value of FTO mRNA and ALKBH5 mRNA Levels in Placental Villi of Patients with Recurrent Miscarriage
文章编号:
1671-7414（2023）06-071-05
作者:
陈 静; 邓健美; 周瑞娴; 黄真轩; 郑晓芳
（深圳市中西医结合医院产科，广东深圳 518104）
Author(s):
CHEN Jing; DENG Jianmei; ZHOU Ruixian; HUANG Zhenxuan; ZHENG Xiaofang
（Department of Obstetrics, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Guangdong Shenzhen 518104，China）
关键词:
目的 探讨胎盘绒毛组织中肥胖相关蛋白（fat mass and obesity associated protein; FTO）mRNA 和AlkB 同源蛋白5（Alk B homologous 5; ALKBH5）mRNA 水平与复发性流产的相关性。方法 选取2019 年12 月～ 2021 年1 月深圳市中西医结合医院产科接收的98 例复发性流产患者为复发性流产组; 同期选取正常早孕人工流产者96 例作为对照组。比较两组一般资料; 采用实时荧光定量PCR（qRT-PCR）法检测两组受试者胎盘绒毛组织FTO m
分类号:
R714.21；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.06.013
文献标志码:
A
摘要:
目的 探讨胎盘绒毛组织中肥胖相关蛋白（fat mass and obesity associated protein，FTO）mRNA 和AlkB 同源蛋白5（Alk B homologous 5，ALKBH5）mRNA 水平与复发性流产的相关性。方法 选取2019 年12 月～ 2021 年1 月深圳市中西医结合医院产科接收的98 例复发性流产患者为复发性流产组，同期选取正常早孕人工流产者96 例作为对照组。比较两组一般资料；采用实时荧光定量PCR（qRT-PCR）法检测两组受试者胎盘绒毛组织FTO mRNA 和ALKBH5mRNA 水平；采用Pearson 法分析复发性流产患者胎盘绒毛组织FTO mRNA 与ALKBH5 mRNA 水平相关性；采用单因素及多因素Logistic 回归分析影响复发性流产的因素。结果 复发性流产组胎盘绒毛组织FTO mRNA（0.72±0.16），ALKBH5 mRNA（0.64±0.18）水平低于对照组（1.02±0.24，1.01±0.21），差异均有统计学意义（t=10.263，13.185，均P ＜ 0.05）。复发性流产患者胎盘绒毛组织FTO mRNA 与ALKBH5 mRNA 水平呈正相关（r=0.537，P ＜ 0.05）。流产次数＞ 3 次的复发性流产患者胎盘绒毛组织FTO mRNA（0.40±0.12），ALKBH5 mRNA（0.47±0.11）水平低于流产次数3 次患者（0.86±0.18，0.72±0.14），差异具有统计学意义（t=12.615，8.561，均P ＜ 0.05）。单因素Logistic 回归分析结果显示，孕囊大小为复发性流产发生的危险因素[OR（95%CI）：2.025（1.468 ～ 2.794），P ＜ 0.05]，FTO mRNA，ALKBH5 mRNA为复发性流产发生的保护因素[OR（95%CI）：0.730（0.548 ～ 0.972）；0.655（0.492 ～ 0.873），均P ＜ 0.05]。多因素Logistic 回归分析结果显示，FTO mRNA 和ALKBH5 mRNA 为复发性流产发生的独立保护因素[OR（95%CI）：0.674（0.519 ～ 0.874），0.541（0.392 ～ 0.747），均P ＜ 0.05]。结论 复发性流产患者胎盘绒毛组织FTO mRNA 和ALKBH5 mRNA 低表达，二者为复发性流产发生的独立保护因素。
Abstract:
Objective To investigate the correlation between the levels of fat mass and obesity associated protein (FTO) mRNA and AlkB homologues 5 (ALKBH5) mRNA in placental villi tissues and recurrent miscarriage. Methods From December 2019 to January 2021, 98 patients with recurrent miscarriage received in Obstetrics Department of Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine were selected as the research objects (recurrent miscarriage group), and 96 patients with normal early pregnancy induced abortion were selected as control (control group) during the same period. The general data of the two groups were compared; the levels of FTO mRNA and ALKBH5 mRNA in the placental villi tissues of the two groups of subjects were detected by real-time fluorescent quantitative PCR (qRT-PCR). The correlation between FTO mRNA and ALKBH5 mRNA levels in the placental villi of patients with recurrent miscarriage was detected by Pearson method. The factors affecting recurrent miscarriage were detected by single factor and multivariate Logistic regression. Results The levels of FTO mRNA (0.72±0.16) and ALKBH5 mRNA (0.64±0.18) in placental villi in the recurrent miscarriage group were lower than those in the control group（1.02±0.24，1.01±0.21）, and the differences were statistically significant (t=10.263,13.185, all P <0.05). There was a positive correlation between FTO mRNA and ALKBH5 mRNA levels in the placental villi of patients with recurrent miscarriage (r=0.537, P<0.05). The levels of FTO mRNA(0.40±0.12) and ALKBH5 mRNA ( 0.47±0.11) in the placental villous tissue of patients with recurrent miscarriage more than 3 miscarriages were lower than those of patients with 3 miscarriages（0.86±0.18，0.72±0.14）, and the differences were statistically significant (t=12.615,8.561, all P <0.05). The results of univariate logistic regression analysis showed that the gestational sac size was a risk factor affecting the occurrence of recurrent miscarriage [OR(95%CI):2.025(1.468 ～ 2.794), P<0.05], and FTO mRNA and ALKBH5 mRNA were protective factors affecting the occurrence of recurrent miscarriage [OR(95%CI): 0.730(0.548 ～ 0.972), 0.655(0.492 ～ 0.873), all P<0.05]. Multivariate Logistic regression analysis showed that FTO mRNA and ALKBH5 mRNA were independent protective factors affecting the occurrence of recurrent miscarriage [OR(95%CI):0.674(0.519 ～ 0.874), 0.541(0.392 ～ 0.747), all P<0.05]. Conclusion Low expression of FTO mRNA and ALKBH5 mRNA in placental villi of patients with recurrent abortion, they are independent protective factors of recurrent abortion.

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备注/Memo

备注/Memo:
作者简介：陈静（1985-），女，本科，主治医师，研究方向：复发性流产的临床研究，E-mail：gdyxywkn@126.com。
更新日期/Last Update: 2023-11-15