[1]李文静,刘凌云,解 媛,等.产单核细胞李斯特菌分子分型技术的最新研究进展[J].现代检验医学杂志,2023,38(06):200-204.[doi:10.3969/j.issn.1671-7414.2023.06.036]
　LI Wenjing,LIU Lingyun,XIE Yuan,et al.Advances in Molecular Typing Techniques of Listeria Monocytogenes[J].Journal of Modern Laboratory Medicine,2023,38(06):200-204.[doi:10.3969/j.issn.1671-7414.2023.06.036]
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产单核细胞李斯特菌分子分型技术的最新研究进展()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年06期
页码:
200-204
栏目:
综述
出版日期:
2023-11-15

文章信息/Info

Title:
Advances in Molecular Typing Techniques of Listeria Monocytogenes
文章编号:
1671-7414（2023）06-200-05
作者:
李文静1; 刘凌云2; 解 媛2; 史 简1; 李 雪1; 陆书华2
（1. 济宁医学院临床医学院，山东济宁 272067；2. 济宁医学院附属医院检验科，山东济宁 272000）
Author(s):
LI Wenjing1;  LIU Lingyun2;  XIE Yuan2;  SHI Jian1;  LI Xue1;  LU Shuhua2
（1.School of Clinical Medicine, Jining Medical University, Shandong Jining 272067, China；2.Department of Clinical Laboratory, the Affiliated Hospital of Jining Medical College, Shandong Jining 272000, China）
关键词:
产单核细胞李斯特菌; 分子分型技术; 食源性疾病; 追踪溯源
分类号:
R446.5；R378.994
DOI:
10.3969/j.issn.1671-7414.2023.06.036
文献标志码:
A
摘要:
随着科学技术的发展与成熟，分子分型技术备受关注，其中，脉冲场凝胶电泳及全基因组测序已成为多种细菌分型的金标准，而基于电泳的分子分型技术、多位点序列分型、多毒力位点序列分型、分子血清分型也各有优缺点和适用范围。该文拟对产单核细胞李斯特菌分子分型技术进行详细阐述，旨在为临床李斯特菌病的暴发流行、同源性分析提供研究方法，为食源性疾病的监测、预警、风险评估及溯源提供参考依据。
Abstract:
With the development and maturity of science and technology, molecular typing technology has attracted much attention, among which, pulsed field gel electrophoresis and whole genome sequencing have become the gold standard for a variety of bacterial typing, and electrophoretic-based molecular typing technology, multilocus sequence typing, multi-virulence site sequence typing, and molecular serotyping also have their advantages and disadvantages and applicable ranges. In this paper, the molecular typing technique of Listeria Monocytogenes was described in detail, aiming to provide research methods for the outbreak and homology analysis of clinical Listeria Monocytogenes, and provide reference basis for monitoring, warning, risk assessment, and traceability of foodborne diseases.

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备注/Memo

备注/Memo:
基金项目： 山东省医药卫生科技发展计划项目（202211000699）：肺炎克雷伯菌毒力特征与耐药性的研究；济宁市重点研发计划（2021YXNS027）：临床分离株单核细胞增生李斯特菌的流行及分子特征。
作者简介：李文静(1996-)，女，硕士研究生在读，检验医师（初级），研究方向：细菌鉴定及耐药分析，E-mail：2960741731@qq.com。
通讯作者：陆书华 (1981-)，女，副主任医师，硕士生导师，主要从事病原微生物学研究，E-mail：lushuhua010515@126.com。
更新日期/Last Update: 2023-11-15