[1]张丽蓉,林 艾,杨 丽.缺氧缺血性脑病新生儿血清miR-139-5p，HDAC4和GFAP表达水平及其临床价值研究[J].现代检验医学杂志,2024,39(01):55-60.[doi:10.3969/j.issn.1671-7414.2024.01.010]
　ZHANG Lirong,LIN Ai,YANG Li.Study on the Expression Levels of Serum miR-139-5p, HDAC4 and GFAP in Neonates with Hypoxic-ischemic Encephalopathy and Their Clinical Value[J].Journal of Modern Laboratory Medicine,2024,39(01):55-60.[doi:10.3969/j.issn.1671-7414.2024.01.010]
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缺氧缺血性脑病新生儿血清miR-139-5p，HDAC4和GFAP表达水平及其临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
55-60
栏目:
论著
出版日期:
2024-01-15

文章信息/Info

Title:
Study on the Expression Levels of Serum miR-139-5p, HDAC4 and GFAP in Neonates with Hypoxic-ischemic Encephalopathy and Their Clinical Value
文章编号:
1671-7414（2024）01-055-06
作者:
张丽蓉; 林 艾; 杨 丽
（广元市中心医院新生儿科，四川广元 628000）
Author(s):
ZHANG Lirong;  LIN Ai;  YANG Li
（Department of Neonatology，Guangyuan Central Hospital, Sichuan Guangyuan 628000, China）
关键词:
缺氧缺血性脑病; 微小核糖核酸-139-5p; 组蛋白去乙酰化酶4; 胶质纤维酸性蛋白
分类号:
R722.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.01.010
文献标志码:
A
摘要:
目的　分析血清微小核糖核酸（micro RNA，miR）-139-5p，组蛋白去乙酰化酶4（histone deacetylase 4，HDAC4） 和胶质纤维酸性蛋白（glialfibrillary acidic protein，GFAP） 与新生儿缺氧缺血性脑病（hypoxic-ischemicencephalopathy，HIE）脑损伤严重程度的关系。方法　选取2017 年1 月～ 2022 年3 月广元市中心医院分娩的HIE 新生儿72 例为研究对象（研究组）；同期健康的足月新生儿75 例为对照组。实时荧光定量PCR 检测血清中miR-139-5p，HDAC4 表达水平。酶联免疫吸附法（ELISA）检测血清GFAP 水平。Logistic 回归分析影响HIE 患儿重度脑损伤发生的因素。结果　与对照组相比，研究组血清GFAP（1.30±0.37ng/L vs 0.50±0.15ng/L），HDAC4 相对表达水平（2.05±0.39 vs 1.02±0.21）升高，miR-139-5p 相对表达水平（0.63±0.14 vs 1.01±0.22）和NBNA 评分（33.20±1.43分 vs 39.85±2.23 分）降低，差异具有统计学意义（t=17.304，20.046，12.436，21.424，均P<0.05）；与轻中度组相比，重度组血清GFAP（1.61±0.47ng/L vs 1.16±0.33ng/L），HDAC4（2.43±0.37 vs 1.87±0.40）相对表达水平升高，miR-139-5p 相对表达水平（0.38±0.10 vs 0.74±0.16）和NBNA 评分（30.52±1.54 分 vs 34.46±1.38 分）降低，差异具有统计学意义（t=4.690，5.669，9.900，10.884，均P<0.05）。Logistic 回归分析显示，miR-139-5p 低表达，HDAC4 高表达，低NBNA 评分，低出生后1 min 内Apgar 评分是影响HIE 患儿重度脑损伤发生的危险因素（Wald χ2=5.772 ～ 6.969，OR=1.519 ～ 1.709，均P ＜ 0.05）。Pearson 分析显示，血清miR-139-5p 表达水平与GFAP， HDAC4 呈负相关（r=-0.416，-0.579，均P ＜ 0.05），血清HDAC4 表达水平与GFAP 呈正相关（r= 0.437，P ＜ 0.05）。Spearman 分析显示，血清miR-139-5p 表达水平与NBNA评分、出生后1 min内Apgar 评分、出生后5 min内Apgar 评分呈正相关（r= 0.398，0.367，0.348，均P ＜ 0.05）；血清HDAC4 表达水平与NBNA 评分、出生后1 min 内Apgar 评分、出生后5 min 内Apgar 评分呈负相关（r=-0.364，-0.345，-0.332，均P ＜ 0.05）。结论　HIE 患儿血清中miR-139-5p 表达降低，HDAC4 表达升高，miR-139-5p，HDAC4 与HIE 患儿脑损伤严重程度有关。
Abstract:
Objective　To analyze the relationship between serum micro RNA（miR）139-5p，histone deacetylase 4 (HDAC4) and glial fibrillary acidic protein (GFAP) and the severity of brain injury in neonatal hypoxic-ischemic encephalopathy (HIE). Methods　From January 2017 to March 2022, 72 HIE neonates born in Guangyuan Central Hospital were collected as research objects (study group)，while 75 healthy full-term newborns were the control group. The expression levels of miR-139-5p and HDAC4 in serum were detected by real-time fluorescence quantitative PCR. ELISA was applied to detect serum GFAP level. Binary logistic regression was applied to analyze the factors affecting the occurrence of severe brain injury in children with HIE. Results　Compared with the control group，the serum GFAP(1.30±0.37ng/L vs 0.50±0.15 ng/L) and HDAC4 relative expression level (2.05±0.39 vs 1.02±0.21) in the study group were increased，the relative expression level of miR-139-5p (0.63±0.14 vs 1.01±0.22) and the NBNA score (33.20±1.43 score vs 39.85±2.23 score) was decreased, the differences were statistically significant (t=17.304，20.046，12.436，21.424，all P<0.05). Compared with the mild to moderate group, the serum GFAP level(1.61±0.47ng/L vs 1.16±0.33ng/L), HDAC4 relative expression level(2.43±0.37 vs 1.87±0.40), miR-139- 5p(0.38±0.10 vs 0.74±0.16) and NBNA score(30.52±1.54 score vs 34.46±1.38 score) relative expression level in the severe group were increased, and the differences were statistically significant（t=4.690, 5.669, 9.900, 10.884, all P<0.05）.Logistic regression analysis showed that low expression of miR-139-5p, high expression of HDAC4, low NBNA score and low Apgar score within 1 min after birth were risk factors for severe brain injury in HIE children （Wald χ2=5.772 ～ 6.969, OR=1.519 ～ 1.709, all P ＜ 0.05）. Pearson analysis showed that the expression level of serum miR-139-5p was negatively correlated with GFAP, HDAC4（r= -0.416, -0.579, all P ＜ 0.05）, while the expression level of serum HDAC4 was positively correlated with GFAP（r= 0.437, P ＜ 0.05）. Spearman analysis showed that the expression level of serum miR-139-5p was positively correlated with NBNA score, Apgar score within 1 min after birth, and Apgar score within 5 min after birth（r= 0.398, 0.367, 0.348, all P ＜ 0.05）.Serum HDAC4 expression level was negatively correlated with NBNA score, Apgar score within 1 min after birth, and Apgar score within 5 min after birth（r=-0.364, -0.345, -0.332, all P ＜ 0.05）. Conclusion　The expression of miR-139-5p in the serum of children with HIE was decreased, and the expression of HDAC4 was increased, miR- 139-5p and HDAC4 were associated with the severity of brain injury in children with HIE.

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备注/Memo

备注/Memo:
基金项目：2017 年四川省卫生和计划生育课题资助项目（17PJ400）: 超低频经颅磁刺激对 AD 模型大鼠认知功能影响及机制探讨。
作者简介：张丽蓉（1975-），女，本科，副主任医师，研究方向：新生儿疾病，E-mail：zhanglr1975r@163.com。
更新日期/Last Update: 2024-01-15