[1]何成山,刘 洋,徐 正,等.内源性因素对抗体夹心免疫法检测血清心肌肌钙蛋白I的干扰及解决方案研究进展[J].现代检验医学杂志,2024,39(01):186-191.[doi:10.3969/j.issn.1671-7414.2024.01.035]
　HE Chengshan,LIU Yang,XU Zheng,et al.Research Progress on Interference of Endogenous Factors in Detection of Serum Cardiac Troponin I by Sandwich Antibody Immunoassay and Its Solutions[J].Journal of Modern Laboratory Medicine,2024,39(01):186-191.[doi:10.3969/j.issn.1671-7414.2024.01.035]
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内源性因素对抗体夹心免疫法检测血清心肌肌钙蛋白I的干扰及解决方案研究进展()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年01期
页码:
186-191
栏目:
综述
出版日期:
2024-01-15

文章信息/Info

Title:
Research Progress on Interference of Endogenous Factors in Detection of Serum Cardiac Troponin I by Sandwich Antibody Immunoassay and Its Solutions
文章编号:
1671-7414（2024）01-186-06
作者:
何成山1; 刘 洋2; 徐 正1; 蒋秀娣1; 陆志成1
（1. 上海中医药大学附属第七人民医院医学检验科，上海 200137；2. 上海中医药大学，上海 201203）
Author(s):
HE Chengshan1;  LIU Yang2;  XU Zheng1;  JIANG Xiudi1;  LU Zhicheng1
（1.Department of Clinical Laboratory， the Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China； 2.Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China）
关键词:
心肌肌钙蛋白I; 抗体; 夹心免疫; 干扰; 内源性因素
分类号:
R446.6
DOI:
10.3969/j.issn.1671-7414.2024.01.035
文献标志码:
A
摘要:
心肌肌钙蛋白I（cardiac troponin I，cTnI）是临床上诊断心肌损伤的首选血清学标志物。cTnI 检测基于抗体夹心免疫法，不同检测试剂中检测和捕获抗体所针对的cTnI 抗原表位不一致，易造成cTnI 检测结果的异质性。内源性干扰因素如cTnI 自身抗体、异嗜性抗体、类风湿因子等可严重的干扰cTnI 检测结果，影响临床上对心肌损伤类疾病的诊断、治疗及预后判断。该文就cTnI 的抗体夹心免疫检测和内源性因素对cTnI 检测的干扰及解决方案等方面研究进展作一综述，为临床上鉴别诊断cTnI 异常检测结果提供理论依据。
Abstract:
Cardial troponin I (cTnI) is the preferred serological marker for the diagnosis of myocardial injury. cTnI detection is based on antibody sandwich immunoassay. The epitopes of cTnI antigen targeted by detecting and capturing antibodies in different detection reagents are inconsistent, which easily leads to the heterogeneity of cTnI detection results. Endogenous interfering factors such as cTnI autoantibody, heterophile antibody, rheumatoid factor, ect, which can seriously interfere with the results of cTnI detection, and affecting the clinical diagnosis, treatment and prognosis of myocardial injury diseases. In this paper, the research progress of antibody sandwich immunoassay for cTnI and interference of endogenous factors on cTnI detection and solutions are reviewed to provide theoretical basis for differential diagnosis of abnormal cTnI detection results in clinical practice.

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备注/Memo

备注/Memo:
基金项目： 上海市卫生健康委员会科研面上项目（202040165）：循环心肌肌钙蛋白I 自身抗体对心肌肌钙蛋白I 检测的影响及相关机制；浦东新区卫生健康委员会公共卫生学科：预防医学与检验学（PWGw2020-02）。
作者简介：何成山（1993-），男，硕士，研究方向：临床免疫学和分子生物学检验，E-mail：xiaohechengshan@163.com。
通讯作者：陆志成，主任技师，主要从事临床检验方面研究，E-mail：xiaoluzhicheng@126.com。
更新日期/Last Update: 2024-01-15