[1]杨立芬,何建清,尹立新.卵巢癌患者血清LncRNA SNHG11 和HIF-1α 水平表达与癌组织血管生成拟态的关系研究[J].现代检验医学杂志,2024,39(02):28-33.[doi:10.3969/j.issn.1671-7414.2024.02.006]
　YANG Lifen,HE Jianqing,YIN Lixin.Study on the Relationship between Serum LncRNA SNHG11 and HIF-1α Level Expression and Vasculogenic Mimicry of Cancer Tissues in Ovarian Cancer[J].Journal of Modern Laboratory Medicine,2024,39(02):28-33.[doi:10.3969/j.issn.1671-7414.2024.02.006]
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卵巢癌患者血清LncRNA SNHG11 和HIF-1α 水平表达与癌组织血管生成拟态的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
28-33
栏目:
论著
出版日期:
2024-03-15

文章信息/Info

Title:
Study on the Relationship between Serum LncRNA SNHG11 and HIF-1α Level Expression and Vasculogenic Mimicry of Cancer Tissues in Ovarian Cancer
文章编号:
1671-7414（2024）02-028-06
作者:
杨立芬1; 何建清1; 尹立新2
（1. 唐山市妇幼保健院妇产科，河北唐山 063000；2. 唐山市南湖医院外科，河北唐山 063000）
Author(s):
YANG Lifen1;  HE Jianqing1;  YIN Lixin2
(1. Department of Obstetrics, Tangshan Maternal and Child Health Care Hospital, Hebei Tangshan 063000, China; 2. Department of Surgery, Tangshan Nanhu Hospital, Hebei Tangshan 063000, China)
关键词:
卵巢癌; 长链非编码RNA 小核仁RNA 宿主基因11; 缺氧诱导因子-1α; 血管生成拟态
分类号:
R737.31；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.02.006
文献标志码:
A
摘要:
目的 探究卵巢癌（ovarian cancer）患者血清长链非编码RNA（long non-coding RNA，LncRNA）小核仁RNA宿主基因11（small nucleolar RNA host gene 11，SNHG11） 和缺氧诱导因子1α（hypoxia inducible factor-1α，HIF-1α）表达与组织血管生成拟态（vasculogenic mimicry，VM）的关系。方法 以2019 年10 月～ 2023 年1 月于唐山市妇幼保健院收治的116 例卵巢癌患者为研究对象，根据是否形成VM将卵巢癌患者分为VM组（n=51）和无VM组（n=65），另选取同期进行健康体检者50 例为对照组。采用实时荧光定量PCR 法（real-time quantitative PCR，qPCR）检测卵巢癌患者及对照组血清LncRNA SNHG11 和HIF-1α 表达水平；Spearman 相关性分析LncRNA SNHG11，HIF-1α 与VM 形成之间的关系；通过受试者工作特征（receiver operating characteristic, ROC）曲线分析LncRNA SNHG11，HIF-1α 及联合检测对卵巢癌患者形成VM 的诊断价值。结果 与对照组相比，卵巢癌患者VM 组和无VM 组血清LncRNA SNHG11（3.01 ± 0.88，2.21 ± 0.68 vs 12 ± 0.35），HIF-1α（2.16 ± 0.67，1.60 ± 0.44 vs 1.01 ± 0.31）水平均显著升高（t=12.136，9.006；19.890，16.591），且VM 组患者血清LncRNA SNHG11，HIF-1α 水平均显著高于无VM 组（t=8.957，8.595），差异有统计学意义（均P ＜ 0.05）；LncRNA SNHG11，HIF-1α 表达及VM的形成与年龄、组织类型无关（t=1.036，0.976，0.218；1.254，1.390，0.368，均P ＞ 0.05），而与肿瘤大小、FIGO 分期、淋巴结转移及病理分级有关（t=5.351，5.186，13.264；5.465，5.227，10.898；6.063，6.016，5.374；4.030，5.871，5.509，均P ＜ 0.05）；Spearman 相关性分析显示，LncRNA SNHG11，HIF-1α 与VM 的生成均呈显著正相关（r=0.560，0.494，均P ＜ 0.05）；ROC 曲线结果显示，血清LncRNA SNHG11，HIF-1α 单独诊断卵巢癌患者形成VM 的曲线下面积（area under the curve，AUC）分别为0.860，0.824，其敏感度分别为80.4%，75.6%，特异度分别为58.9%，51.9%；两者联合诊断卵巢癌患者形成VM的AUC 为0.941，其敏感度、特异度分别为92.2%，79.9%。结论 LncRNA SNHG11 和HIF-1α 的异常表达与卵巢癌患者形成VM 密切相关，两者可作为潜在判断VM 的生物学指标。
Abstract:
Objective To investigate the relationship between the expression of long non-coding RNA (LncRNA) small nucleolar RNA host gene 11 (SNHG11) and hypoxia inducible factor(HIF)-1α and angiogenesis mimicry (VM) in ovarian cancer. Methods A total of 116 ovarian cancer patients admitted to Tangshan Maternal and Child Health Care Hospital from October 2019 to January 2023 were regarded as the research subjects. Based on whether VM had formed, ovarian cancer patients were grouped into VM group (n=51) and non VM group (n=65). Another 50 partients who underwent health examinations during the same period were regarded as the control group. Real-time fluorescence quantitative PCR（qPCR）was applied to detect the expression levels of LncRNA SNHG11 and HIF-1α in serum of ovarian cancer patients and control groups. Spearman correlation was applied to detect the relationship between LncRNA SNHG11, HIF-1α, and VM formation. The diagnostic value of LncRNA SNHG11, HIF-1α, and their combined detection in the formation of VM in ovarian cancer patients was analyzed using the receiver operating characteristic (ROC) curve. Results Compared with the control group, the levels of serum LncRNA SNHG11 ( 3.01 ± 0.88, 2.21 ± 0.68 vs 1.12 ± 0.35) and HIF-1α ( 2.16 ± 0.67, 1.60 ± 0.44 vs 1.01 ± 0.31) in ovarian cancer patients with VM group and non VM group were increased (t=12.136, 9.006; 19.890, 16.591, all P ＞ 0.05), the levels of serum LncRNA SNHG11 and HIF-1αin the VM group were obviously higher than those in the non VM group(t=8.957, 8.595), and the differences were statistically significant(all P ＜ 0.05). The expression of LncRNA SNHG11, HIF-1α, and the formation of VM were not related to age and tissue type （t=1.036，0.976，0.218；1.254，1.390，0.368，all P ＞ 0.05）, but were related to tumor size, FIGO staging, lymph node metastasis, and pathological grading （t=5.351，5.186，13.264；5.465，5.227， 10.898；6.063，6.016，5.374；4.030，5.871，5.509，all P ＜ 0.05）. Spearman correlation analysis showed that there were obvious positive correlations between LncRNA SNHG11, HIF-1α, and VM generation (r=0.560, 0.494, all P<0.05). ROC curve results showed that the areas under the curve (AUCs) of serum LncRNA SNHG11 and HIF-1α for diagnosing VM formation in ovarian cancer patients were 0.860 and 0.824, respectively, with sensitivity of 80.4% and 75.6%, specificity of 58.9% and 51.9%, respectively. The AUC of VM formation in ovarian cancer patients diagnosed by the combination of the two was 0.941, with sensitivity and specificity were 92.2% and 79.9%, respectively. Conclusion The abnormal expressions of LncRNA SNHG11 and HIF-1α were closely related to the formation of VM in ovarian cancer patients, and both may serve as potential biological indicators for judging VM.

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备注/Memo

备注/Memo:
基金项目：河北省医学科学研究课题计划（编号：20191534）：壳多糖酶3 样蛋白1 对卵巢癌SKOV3 细胞血管生成拟态形成的影响。
作者简介：杨立芬（1981-），女，硕士研究生，副主任医师，研究方向：妇科肿瘤与内分泌，E-mail：c1636hk2@163.com。
更新日期/Last Update: 2024-03-15