[1]林 艳,但汉良,余 扬,等.慢性心力衰竭患者血清miR-122 和miR-558 水平表达与心功能及心率变异性的相关性研究[J].现代检验医学杂志,2024,39(03):37-41+114.[doi:10.3969/j.issn.1671-7414.2024.03.006]
　LIN Yan,DAN Hanliang,YU Yang,et al.Correlation between Serum miR-122 and miR-558 Levels and Cardiac Function and Heart Rate Variability in Patients with Chronic Heart Failure[J].Journal of Modern Laboratory Medicine,2024,39(03):37-41+114.[doi:10.3969/j.issn.1671-7414.2024.03.006]
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慢性心力衰竭患者血清miR-122 和miR-558 水平表达与心功能及心率变异性的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
37-41+114
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Correlation between Serum miR-122 and miR-558 Levels and Cardiac Function and Heart Rate Variability in Patients with Chronic Heart Failure
文章编号:
1671-7414（2024）03-037-06
作者:
林 艳1; 但汉良1; 余 扬1; 高 鹏1; 黄继良1; 白 杨2
（1. 武汉市东西湖区人民医院心血管内科，武汉 430040；2. 华中科技大学同济医学院附属同济医院心血管内科，武汉 430030）
Author(s):
LIN Yan1;  DAN Hanliang1;  YU Yang1;  GAO Peng1;  HUANG Jiliang1;  BAI Yang2
(1. Department of Cardiovascular Medicine, Wuhan Dongxihu District People’s Hospital, Wuhan 430040, China；2. Department of Cardiovascular Medicine, Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China)
关键词:
慢性心力衰竭; 微小RNA-122; 微小RNA-558; 心功能; 心率变异性
分类号:
R541.6；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.03.006
文献标志码:
A
摘要:
目的 探讨慢性心力衰竭（chronic heart failure，CHF）患者血清微小RNA-122（miR-122）和微小RNA-558（miR-558）水平表达与心功能及心率变异性的关系。方法 收集2019 年1 月～ 2022 年1 月武汉市东西湖区人民医院收治的100 例慢性心力衰竭患者作为研究组，同期在该院进行体检的健康者100 例作为对照组。采用实时荧光定量PCR法检测两组血清miR-122 和miR-558 表达水平；比较两组心率变异性指标及心功能指标；采用Pearson 法分析慢性心力衰竭患者血清miR-122，miR-558 表达水平与心率变异性指标、心功能指标的相关性；用Logistic 回归分析影响慢性心力衰竭的危险因素。结果 与对照组相比，慢性心力衰竭患者血清miR-122（0.86±0.19 vs 1.07±0.16）及心率变异性指标、左室射血分数（LVEF）均明显降低（t=3.844 ～ 15.448）， miR-558（1.28±0.27 vs 1.02±0.12）表达水平及左室舒张末期容积指数（LVEDVI）、左室收缩末期容积指数（LVESVI）均明显升高（t=6.794 ～ 9.358），差异具有统计学意义（均P ＜ 0.05）；不同心功能分级I，II 级组患者血清miR-122（1.19±0.23，1.04±0.21）表达水平显著高于III，IV 级组（0.68±0.16，0.53±0.15），miR-558（0.93±0.24，1.21±0.26）表达水平显著低于III，IV 级组（1.42±0.27，1.64±0.29），差异具有统计学意义（qmiR-122=3.751~14.568，qmiR-558=3.929~11.194，均P ＜ 0.05）。慢性心力衰竭患者血清miR-122 与心率变异性指标、LVEF 均呈正相关性（r=0.337 ～ 0.573），与LVEDVI，LVESVI 呈负相关性（r=-0.385，-0.323），血清miR-558 与心率变异性指标、LVEF 均呈负相关性（r=-0.646 ～ -0.246），与LVEDVI，LVESVI 呈正相关性（r=0.528，0.547）；血清miR-122[OR(95%CI)=0.528(0.328 ～ 0.850)]，miR-558[OR(95%CI)=2.845(1.364 ～ 5.933)] 是慢性心力衰竭的影响因素（P ＜ 0.05）。结论 慢性心力衰竭患者血清miR-122，miR-558 表达水平与心功能、心率变异性密切相关，且血清miR-122 和miR-558 是慢性心力衰竭的影响因素。
Abstract:
Objective To explore the relationship between serum microRNA (miR) -122 and microRNA (miR) -558 levels and cardiac function and heart rate variability in patients with chronic heart failure（CHF）. Methods A total of 100 patients with chronic heart failure who were hospitalized in Wuhan Dongxihu District Poeple’s Hospital from January 2019 to January 2022 were regarded as the study group, and 100 healthy individuals who underwent physical examinations in the hospital during the same period served as the control group. The real-time fluorescence quantitative PCR method was applied to detect the expression levels of serum miR-122 and miR-558 in two groups. The heart rate variability and cardiac function indicators of the two groups were compared. Pearson method was applied to analyze the correlation between serum miR-122 and miR-558 expression levels, and heart rate variability and cardiac function indicators in patients with chronic heart failure. Logistic regression was applied to analyze the risk factors affecting chronic heart failure. Results Compared with the control group, the serum miR-122 (0.86±0.19 vs 1.07±0.16), heart rate variability indicators, and left ventricular ejection fraction (LVEF) of patients with chronic heart failure were decreased（t=3.844 ～ 15.448）, and the expression level of miR-558 (1.28±0.27 vs 1.02±0.12) and left ventricular end diastolic volume index (LVEDVI), left ventricular end systolic volume index (LVESVI) were increased （t=6.794 ～ 9.358），and the differences were statistically significant(all P<0.05). The expression level of serum miR-122 (1.19±0.23, 1.04±0.21) in patients with functional grades I to II was higher than that in patients with grades III to IV (0.68±0.16, 0.53±0.15), while the expression level of miR-558 (0.93±0.24, 1.21±0.26) was lower than that in patients with grade III-IV (1.42±0.27, 1.64±0.29), with significant differences (qmiR-122=3.751~14.568, qmiR-558=3.929~11.194, all P<0.05).The serum miR-122 level in patients with chronic heart failure was positively correlated with heart rate variability indicators and LVEF（r=0.337 ～ 0.573），but negatively correlated with LVEDVI and LVESVI （r=-0.385，-0.323）. Serum miR-558 was negatively correlated with heart rate variability indicators and LVEF（r=-0.646 ～ -0.246）, but positively correlated with LVEDVI and LVESVI（r=0.528，0.547）. Serum miR-122 [OR (95%CI) = 0.528 (0.328 ～ 0.850)] and miR-558 [OR (95%CI) = 2.845 (1.364~5.933)] were influencing factors for chronic heart failure (P<0.05). Conclusion The expression levels of serum miR-122 and miR-558 in patients with chronic heart failure were closely related to heart rate variability and cardiac function, indicating that serum miR-122 and miR-558 may be influencing factors of chronic heart failure.

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备注/Memo

备注/Memo:
作者简介：林艳（1986-），女，主治医师，研究方向：慢性心力衰竭，E-mail：loiii67@163.com。
通讯作者：白杨（1984-），男，主治医师，研究方向：慢性心力衰竭，E-mail：lbyby23@126.com。
更新日期/Last Update: 2024-05-15