[1]孔 滔a,饶 舜b,朱 伟c,等.系统性红斑狼疮患者外周血T 淋巴细胞Mg2+ 转运蛋白1 水平表达及临床价值研究[J].现代检验医学杂志,2024,39(03):157-163.[doi:10.3969/j.issn.1671-7414.2024.03.027]
　KONG Taoa,RAO Shunb,ZHU Weic,et al.Study on the Expression Level of Magnesiun Transporter Protein 1 in Peripheral Blood T Lymphocytes of Patients with Systemic Lupus Erythematosus and Its Clinical Value[J].Journal of Modern Laboratory Medicine,2024,39(03):157-163.[doi:10.3969/j.issn.1671-7414.2024.03.027]
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系统性红斑狼疮患者外周血T 淋巴细胞Mg2+ 转运蛋白1 水平表达及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
157-163
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Study on the Expression Level of Magnesiun Transporter Protein 1 in Peripheral Blood T Lymphocytes of Patients with Systemic Lupus Erythematosus and Its Clinical Value
文章编号:
1671-7414（2024）03-157-07
作者:
孔 滔a; 饶 舜b; 朱 伟c; 玉坎哈a; 余 超a
（西双版纳傣族自治州人民医院a. 医学检验科；b. 病理科；c. 科教科，云南景洪 666100）
Author(s):
KONG Taoa;  RAO Shunb;  ZHU Weic;  YU Kanhaa;  YU Chaoa
（a. Department of Laboratory Medicine; b. Department of Pathology；c. Department of Science and Education, the People’s Hospital of Xishuangbanna Dai Autonomous Prefecture, Yunnan Jinghong 666100, China）
关键词:
系统性红斑狼疮; Mg2+ 转运蛋白1; 系统性红斑狼疮疾病活动指数; 外周血T 淋巴细胞
分类号:
R593.241；R446.6
DOI:
10.3969/j.issn.1671-7414.2024.03.027
文献标志码:
A
摘要:
目的 探讨Mg2+ 转运蛋白1（magnesium transporter protein 1，MagT1）在系统性红斑狼疮（systemic lupuserythematosus，SLE）患者外周血T 淋巴细胞中的表达情况，并阐述其临床意义。方法 选取西双版纳傣族自治州人民医院2019 年１月～ 2021 年6 月收治的79 例SLE 患者作为研究对象，依据SLE 疾病活动指数（SLE disease activityindex，SLEDAI）评分将SLE 患者分为中重度组（SLEDAI ≥ 10，n=32）和轻度组（SLEDAI ＜ 10，n=47），另选取同期体检健康者40 例作为对照组。记录患者临床生化和血清免疫相关指标水平；采用实时定量PCR（quantitative realtimePCR，qRT-PCR）法和Western Blot 法检测患者外周血T 淋巴细胞中MagT1 mRNA 和蛋白表达情况；Pearson 相关性分析外周血T 淋巴细胞MagT1 蛋白与生化免疫相关指标表达的关系；绘制受试者工作特征（receiver operatingcharacteristic，ROC）曲线分析外周血T 淋巴细胞MagT1 蛋白表达对SLE 及其严重程度的诊断价值。结果 与对照组比较，轻度组和中重度组患者外周血T 淋巴细胞中MagT1 mRNA [0.65 (0.36, 0.99)，0.23 (0.07, 0.36) vs 1.20 (0.83，1.37)]和MagT1 蛋白[0.35 (0.22，0.42)，0.22 (0.15，0.27) vs 0.53 (0.40, 0.63)] 表达水平明显降低，差异具有统计学意义（Z=5.247，7.078；5.128，7.257，均P<0.05）；与轻度组比较，中重度组患者外周血T 淋巴细胞中MagT1 mRNA 和蛋白表达水平明显降低，差异具有统计学意义（Z=5.169，3.599，均P<0.05）。Pearson 相关性结果显示，外周血T 淋巴细胞MagT1蛋白表达水平与血清血红蛋白（hemoglobin，HGB）及补体3（complement 3，C3）和补体4（complement 4，C4）水平呈正相关（r=0.496，0.637，0.588，均P<0.05）；与红细胞沉降速率（erythrocyte sedimentation rate，ESR）、超敏C 反应蛋白（hypersensitive-C reactive protein，hs-CRP）、免疫球蛋白（immunoglobulin G，IgG）水平和SLEDAI 呈负相关（r=-0.598，-0.476，-0.646，-0.514，均P<0.05）。ROC 曲线结果显示，外周血T 淋巴细胞MagT1 蛋白表达水平诊断SLE 曲线下面积为0.893（95%CI：0.838 ～ 0.948），敏感度和特异度分别为90.0%，67.1%，诊断SLE 严重程度曲线下面积为0.739（95%CI：0.631 ～ 0.848），敏感度和特异度分别为57.4%，84.4%。结论 外周血T 淋巴细胞MagT1 在SLE 患者中表达下调，且与SLE 严重程度呈负相关，对SLE 诊断和病情严重程度具有一定的诊断价值。
Abstract:
Objective To investigate the expression of magnesiun transporter 1 protein (MagT1) in peripheral blood T lymphocytes of patients with systemic lupus erythematosus (SLE), and elucidate its clinical significance. Methods A total of 79 SLE patients admitted to the People’s Hospital of Xishuangbanna Dai Autonomous Prefecture from January 2019 to June 2021 were selected as the study subjects, and SLE patients were divided into moderate to severe group (SLEDAI≥10, n=32) and mild group (SLEDAI<10, n=47), according to SLE disease activity index (SLEDAI) score. Another 40 healthy patients who underwent physical examinations during the same period were selected as control group. The levels of clinical biochemical and serum immune-related indexes were recorded. The expressions of MagT1 mRNA and protein in peripheral blood T lymphocytes were detected by quantitative real-time PCR (qRT-PCR) and Western Blot methods. The correlation between the expression of MagT1 protein in peripheral blood T lymphocytes and biochemical and immunity-related indexes was analyzed by Pearson correlation analysis. Receiver working characteristic (ROC) curve was drawn to analyze the diagnostic value of MagT1 protein expression of peripheral blood T lymphocytes in diagnosing SLE and its severity. Results Compared with the control group, the expression levels of MagT1 mRNA [0.65 (0.36, 0.99)，0.23 (0.07, 0.36) vs 1.20 (0.83，1.37)]and MagT1 protein [0.35 (0.22, 0.42), 0.22 (0.15，0.27) vs 0.53 (0.40, 0.63)]of peripheral blood T lymphocytes in the mild group and the moderate to severe group were reduced, with significant differences (Z=5.247, 7.078; 5.128, 7.257, all P<0.05). Compared with the mild group, the expression levels of MagT1 mRNA [0.65 (0.36, 0.99) vs 0.23 (0.07, 0.36)] and protein [0.35 (0.22, 0.42) vs 0.22 (0.15, 0.27)] of peripheral blood T lymphocytes in the moderate to severe group were reduced, with significant differences (Z=5.169, 3.599, all P<0.05). Pearson correlation results showed that MagT1 protein expression in peripheral blood T lymphocytes was positively correlated with serum hemoglobin (HGB), complement 3 (C3) and C4 levels (r=0.496, 0.637, 0.588, all P<0.05), but was negatively correlated with serum erythrocyte sedimentation rate (ESR), hypersensitive C-reactive protein (hs-CRP), IgG levels and SLEDAI (r=-0.598, -0.476, -0.646, -0.514, all P<0.05). The results of the ROC curve showed that the area under the curve in diagnostic SLE for the expression level of peripheral blood T lymphocyte MagT1 protein was 0.893 (95%CI: 0.838 ～ 0.948), with a sensitivity and a specificity of 90.0% and 67.1%, respectively，and the area under the curve in diagnostic SLE severity was 0.739 (95%CI:0.631 ～ 0.848), with a sensitivity and a specificity of 57.4% and 84.4%，respectively. Conclusion The expressions of peripheral blood T lymphocyte and MagT1 were down regulated in SLE patients, and they had a negative trend with the severity of SLE. They may have a certain diagnostic value for the diagnosis and severity of SLE.

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备注/Memo

备注/Memo:
作者简介：孔滔（1994-），女，本科，检验技师，研究方向：医学检验，E-mail：17708812064@163.com。
通讯作者：余超（1979-），男，本科，副主任检验技师，研究方向：医学检验，E-mail：bnzyyjyk@163.com。
更新日期/Last Update: 2024-05-15