[1]杨蓓蕾a,石 剑b,王燕捷a.子痫前期孕妇血清miR-411-5p 和miR-485-5p 水平检测联合超声血流指标对围生儿结局的预测价值研究[J].现代检验医学杂志,2024,39(03):183-188.[doi:10.3969/j.issn.1671-7414.2024.03.031]
　YANG Beileia,SHI Jianb,WANG Yanjiea.Study on the Predictive Value of Serum miR-411-5p and miR-485-5p Levels Combined with Ultrasound Blood Flow Indicators for Perinatal Outcomes in Preeclampsia Pregnant Women[J].Journal of Modern Laboratory Medicine,2024,39(03):183-188.[doi:10.3969/j.issn.1671-7414.2024.03.031]
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子痫前期孕妇血清miR-411-5p 和miR-485-5p 水平检测联合超声血流指标对围生儿结局的预测价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
183-188
栏目:
检验与临床
出版日期:
2024-05-15

文章信息/Info

Title:
Study on the Predictive Value of Serum miR-411-5p and miR-485-5p Levels Combined with Ultrasound Blood Flow Indicators for Perinatal Outcomes in Preeclampsia Pregnant Women
文章编号:
1671-7414（2024）03-183-06
作者:
杨蓓蕾a; 石 剑b; 王燕捷a
（北京核工业医院 a. 妇产科；b. 超声科，北京 102413）
Author(s):
YANG Beileia; SHI Jianb; WANG Yanjiea
(a. Department of Gynaecology and Obstetrics; b. Department of Medical Ultrasonics, Beijing Nuclear Industry Hospital, Beijing 102413, China)
关键词:
子痫前期; 搏动指数; 阻力指数; 微小核糖核酸-411-5p; 微小核糖核酸-485-5p
分类号:
R714.244；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.03.031
文献标志码:
A
摘要:
目的 分析子痫前期（preeclampsia，PE）孕妇血清微小核糖核酸-411-5p（miR-411-5p）和微小核糖核酸-485-5p（miR-485-5p）水平检测联合超声血流指标对围生儿结局的预测价值研究。方法 选择2020 年1 月～ 2021 年12 月在北京核工业医院产科建卡并行剖宫产术的88 例PE 孕妇为研究对象（PE 组），另选取同期因其它各种原因剖宫产分娩的正常孕妇90 例作为对照组，比较两组间超声血流指标搏动指数（pulsitility index，PI）、阻力指数（resistance index，RI）差异，实时荧光定量PCR（real time fluorescent quantitative PCR，qRT-PCR）法检测两组间血清miR-411-5p 和miR-485-5p 水平并比较，根据PE 患者围生儿结局的不同分为结局良好组与结局不良组，比较组间PI，RI，miR-411-5p 和miR-485-5p 的差异，采用受试者工作特征（receiver operating characteristic，ROC）曲线法分析PI，RI，miR-411-5p，miR-485-5p 以及联合检测对PE 患者围生儿不良结局的预测作用。结果 PE组孕妇RI（0.79 ± 0.08），PI 值（1.82 ± 0.08）高于对照组（0.66 ± 0.06，1.38 ± 0.15），血清miR-411-5p（0.32 ± 0.09），miR-485-5p（0.26 ± 0.03）水平低于对照组（1.01 ± 0.08，1.02 ± 0.09），差异具有统计学意义（t=12.283，24.339，54.091，75.231，均P ＜ 0.001）；结局不良组PE 患者RI（0.83 ± 0.08），PI（1.86 ± 0.09 ）值高于结局良好组（0.70 ± 0.07，1.71 ± 0.07），血清miR-411-5p（0.27 ± 0.02），miR-485-5p（0.24 ± 0.02）水平低于结局良好组（0.45 ± 0.04，0.31 ± 0.04），差异具有统计学意义（t=11.545，12.428，37.840，14.716，均P ＜ 0.001）。超声血流指标RI，PI 预测PE 患者围生儿不良结局的曲线下面积为0.838（敏感度为90.8%，特异度为65.2%）、0.758（敏感度为50.8%，特异度为91.3%）；血清miR-411-5p，miR-485-5p 预测PE 患者围生儿不良结局的曲线下面积为0.830（敏感度为90.8%，特异度为73.9%）、0.769（敏感度为95.4%，特异度为61.9%），四者联合检测预测PE 患者围生儿不良结局的曲线下面积为0.976（敏感度为98.5%，特异度为91.3%）。结论 PE 孕妇血清miR-411-5p 和miR-485-5p 水平降低，血清miR-411-5p 和miR-485-5p 水平联合超声血流指标PI，RI 对PE 孕妇围生期胎儿结局具有预测作用。
Abstract:
Objective To analyze the predictive value of serum micro RNA（miR）-411-5p and micro RNA（miR）-485-5p levels in preeclampsia (PE) pregnant women combined with ultrasound blood flow indicators for perinatal outcomes. Methods A total of 88 pregnant women with PE who were enrolled in the obstetric card and underwent cesarean section in Beijing Nuclear Industry Hospital from January 2020 to December 2021 were selected as the study subjects (PE group). In addition, 90 normal pregnant women who delivered by cesarean section due to other reasons in the same period were regarded as the control group. The differences of pulsitility index (PI) and resistance index (RI) were compared between the two groups. The serum levels of miR-411-5p and miR-485-5p were measured and compared between the two groups by real time fluorescent quantitative PCR (qRT-PCR). According to the different perinatal outcomes of PE patients, they were grouped into good outcome group and poor outcome group. The differences of PI, RI, miR-411-5p and miR-485-5p between the two groups were compared. The predictive effect of PI, RI, miR-411-5p, miR-485-5p and combined detection on the adverse perinatal outcome of PE patients was analyzed by the receiver operating characteristic (ROC) curve method. Results The RI (0.79 ± 0.08) and PI values (1.82 ± 0.08) of pregnant women in the PE group were higher than those in the control group（0.66 ± 0.06，1.38 ± 0.15）, the serum levels of miR-411-5p (0.32 ± 0.09 ) and miR-485-5p (0.26 ± 0.03 ) were lower than those in the control group（1.01 ± 0.08，1.02 ± 0.09）, and the differences were statistically significant (t=12.283, 24.339, 54.091, 75.231, all P<0.001). The RI (0.83 ± 0.08) and PI (1.86 ± 0.09) values of PE patients in the poor outcome group were higher than those in the good outcome group（0.70 ± 0.07，1.71 ± 0.07）, the serum levels of miR-411-5p (0.27 ± 0.02) and miR-485-5p (0.24 ± 0.02) were lower than those in the good outcome group（0.45±0.04，0.31±0.04）, and the differences were statistically significant (t=11.545, 12.428, 37.840, 14.716, all P<0.001). The areas under the curve of ultrasound blood flow index RI and PI to predict the adverse perinatal outcome of PE patients were 0.838 (sensitivity was 90.8%, specificity was 65.2%), and 0.758 (sensitivity was 50.8%, specificity was 91.3%), respectively. The areas under the curve of serum miR-411-5p and miR-485-5p in predicting adverse perinatal outcome of PE patients were 0.830 (sensitivity was 90.8%, specificity was 73.9%), and 0.769 (sensitivity was 95.4%, specificity was 61.9%), respectively. The area under the curve of the four combined tests to predict the adverse perinatal outcome of PE patients was 0.976 (sensitivity was 98.5%, specificity was 91.3%). Conclusion The levels of miR-411-5p and miR-485-5p in serum of PE pregnant women were decreased, and the combination of miR-411-5p and miR-485-5p and ultrasound blood flow indicators PI and RI may predict the perinatal fetal outcome of PE pregnant women.

参考文献/References:

[1] 杨超, 苗丽, 马建彩, 等. 早发型重度子痫前期患者血清SPA,SPD 及Hcy 表达及临床意义[J]. 现代检验医学杂志, 2023, 38(1): 186-190. YANG Chao, MIAO Li, MA Jiancai, et al. Expression and clinical significance of serum SPA, SPD and Hcy in patients with early-onset severe preeclampsia[J].Journal of Modern Laboratory Medicine, 2023, 38(1):186-190.
[2] RANA S, LEMOINE E, GRANGER J P, et al. Preeclampsia: pathophysiology, challenges, and perspectives[J]. Circulation Research, 2019, 124(7):1094-1112.
[3] HILL M, TRAN N. MiRNA interplay: mechanisms and consequences in cancer[J]. Disease Models & Mechanisms, 2021, 14(4): dmm047662.
[4] 徐其艳, 齐成秋, 王李纲, 等. 早发型及晚发型子痫前期miRNA 调控网络的构建及生物信息学分析[J].中国优生与遗传杂志, 2021, 29(11): 1532-1537. XU Qiyan, QI Chengqiu, WANG Ligang, et al. Construction of miRNA regulatory network for early-onset and late-onset preeclampsia based on bioinformatics analysis[J]. Chinese Journal of Birth Health & Heredity, 2021, 29(11): 1532-1537.
[5] 张文琦, 姚春凤, 胡小娜, 等. 母体外周血循环miRNA 和子痫前期的研究进展[J]. 华北理工大学学报(医学版), 2022, 24(3): 241-248. ZHANG Wenqi, YAO Chunfeng, HU Xiaona,et al. Research progress of maternal peripheral blood circulation miRNA and preeclampsia[J].Journal of North China University of Science and Technology(Health Sciences Edition), 2022, 24(3):241-248.
[6] 吴琪瑞, 王艳华, 刘林英, 等. miR-488-3p 在子痫前期胎盘滋养层细胞侵袭和迁移中的作用研究[J]. 实用医学杂志, 2021, 37(5): 601-605. WU Qirui, WANG Yanhua, LIU Linying, et al. Role of miR-488-3p in invasion and migration of trophoblast cells in preeclampsia placenta[J]. Journal of Practical Medicine, 2021, 37(5): 601-605.
[7] 丁海林. 滋养细胞高表达CD81 导致母体Treg/Th17免疫失衡参与子痫前期发病机制研究[D]. 南京：南京大学, 2019. DING Hailin. High expression of CD81 in trophoblasts leads to maternal Treg/Th17 immune imbalance, which is involved in the pathogenesis of preeclampsia[D].Nanjing: Nanjing University, 2019.
[8] 娄源芳, 杨瑞敏, 王义成, 等. 超声评估子痫前期患者胎儿主动脉峡部、脐动脉血流参数及右心室Tei 指数的临床意义[J]. 中国超声医学杂志, 2021,37(7): 793-795. LOU Yuanfang, YANG Ruimin, WANG Yicheng, et al. The clinical significance of ultrasound evaluation of aortic isthmus, umbilical artery blood flow parameters and right ventricular Tei index in fetuses of patients with preeclampsia[J]. Chinese Journal of Ultrasound in Medicine, 2021, 37(7): 793-795.
[9] FAN Xufei, LOU Jianyi, ZHENG Xiujuan, et al. Interference with LncRNA NEAT1 promotes the proliferation, migration, and invasion of trophoblasts by upregulating miR-411-5p and inhibiting PTEN expression[J]. Immunopharmacology and Immunotoxicology, 2021, 43(3): 334-342.
[10] CHEN Jiying, ZHANG Yonggang, TAN Wenqing, et al. Silencing of long non-coding RNA NEAT1 improves Treg/Th17 imbalance in preeclampsia via the miR-485-5p/AIM2 axis[J]. Bioengineered, 2021, 12(1): 8768-8777.
[11] 中华医学会妇产科学分会妊娠期高血压疾病学组.妊娠期高血压疾病诊治指南(2020）[J]. 中华妇产科杂志, 2020, 55(4): 227-238. Hypertensive Disorders in Pregnancy Subgroup,Chinese Society of Obstetrics and Gynecology, Chinese Medical Association. Diagnosis and treatment of hypertension and pre-eclampsia in pregnancy: a clinical practice guideline in China(2020)[J]. Chinese Journal of Obstetrics and Gynecology, 2020, 55(4): 227-238.
[12] 王娟娟, 张莹, 金瑞林, 等. 子痫前期孕妇子宫动脉和胎儿脐动脉血流变化及其与围生儿预后关系分析[J]. 中国临床医生杂志, 2019, 47(9): 1089-1091. WANG Juanjuan, ZHANG Ying, JIN Ruilin, et al. The changes of pregnant women uterine artery and fetal umbilical artery blood flow and the analysis of perinatal prognosis in preeclampsia[J]. Chinese Journal for Clinicians, 2019, 47(9): 1089-1091.
[13] 胡小娜, 郭敏, 熊杰, 等. 孕中期血清PLGF, sFlt-1,sEng, sCD40L 与子痫前期及胎儿不良结局的关系研究[J]. 中国妇产科临床杂志, 2022, 23(1): 53-56. HU Xiaona, GUO Min, XIONG Jie, et al. Relationship between serum P1GF, sFlt-1, sEng, sCD40L and adverse outcomes of preeclampsia and fetus in the second trimester of pregnancy[J]. Chinese Journal of Clinical Obstetrics and Gynecology, 2022, 23(1): 53-56.
[14] NAKASHIMA A, SHIMA T, TSUDA S, et al. Aggrephagy deficiency in the placenta: a new pathogenesis of preeclampsia[J]. International Journal of Molecular Sciences, 2021, 22(5): 2432.
[15] 姜雪, 陆瑞, 何笑. 不同孕龄超声脐动脉血流参数与胎儿窘迫的关系[J]. 中国医药导报, 2022, 19(21):111-114. JIANG Xue, LU Rui, HE Xiao. Relationship between ultrasound umbilical artery blood flow parameters and fetal distress at different gestational ages[J]. China Medical Herald, 2022, 19(21): 111-114.
[16] WANG Zengfang, WANG Pingping, WANG Zengyan,et al. MiRNA-548c-5p downregulates inflammatory response in preeclampsia via targeting PTPRO[J].Journal of Cellular Physiology, 2019, 234(7): 11149-11155.
[17] 佟春晓, 陈海英, 孙曼妮, 等. 子痫前期患者胎盘组织中环状RNA 差异表达的生物信息学分析[J]. 中国医科大学学报, 2021, 50(8): 673-677. TONG Chunxiao, CHEN Haiying, SUN Manni, et al. Bioinformatics analysis-based identification of key circular RNAs in the placentas of patients with preeclampsia[J]. Journal of China Medical University,2021, 50(8): 673-677.
[18] SHI Ziyun, SHE Kaie, LI Hong, et al. MicroRNA-454 contributes to sustaining the proliferation and invasion of trophoblast cells through inhibiting Nodal/ALK7 signaling in pre-eclampsia[J]. Chemico-Biological Interactions, 2019, 298: 8-14.
[19] 张磊, 李国良, 孟帮柱, 等. miR-485-5p 通过靶基因FLOT2 调控甲状腺癌细胞增殖、凋亡的分子机制[J].中国老年学杂志, 2020, 40(15): 3313-3319. ZHANG Lei, LI Guoliang, MENG Bangzhu, et al. Molecular mechanism of miR-485-5p targeted regulating FLOT2 on thyroid cancer cell proliferation and apoptosis[J]. Chinese Journal of Gerontology,2020, 40(15): 3313-3319.
[20] 铁炜炜, 葛芬芬. 氧化苦参碱通过上调miR-485-5p的表达抑制宫颈癌细胞的增殖研究[J]. 现代实用医学, 2022, 34(3): 305-307, F0004. TIE Weiwei, GE Fenfen. Proliferation research of oxymatrine on inhibiting cervical cancer cells by upregulating the expression of miR-485-5p[J]. Modern Practical Medicine, 2022, 34(3): 305-307, F0004.
[21] MAYAYALO K P, HUANG Xiaobo.QIAN Zhu,et al.Low circulating levels of vitancin D may contribute to the occurrence of preeclampsia through deregulation of Trey/Th17 cell ratio[J].American Journal of Reproductive Immunology,2019,82(4):e13168.

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备注/Memo

备注/Memo:
作者简介：杨蓓蕾（1982-），女，本科，副主任医师，研究方向：妇产科，E-mail：x0eg8087@163.com。
通讯作者：石剑（1983-），男，本科，副主任医师，研究方向：妇产科，E-mail：queniccm7458@163.com。
更新日期/Last Update: 2024-05-15