[1]严 双a,杜 娟b,曹 露c,等.冠状动脉粥样硬化性心脏病患者血清miR-24-3p 和MARK4 水平表达与心功能的相关性研究[J].现代检验医学杂志,2024,39(04):10-15.[doi:10.3969/j.issn.1671-7414.2024.04.003]
　YAN Shuanga,DU Juanb,CAO Luc,et al.Study on the Correlation between Serum miR-24-3p and MARK4 Level Expressions and Cardiac Function in Patients with Coronary Atherosclerotic Heart Disease[J].Journal of Modern Laboratory Medicine,2024,39(04):10-15.[doi:10.3969/j.issn.1671-7414.2024.04.003]
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冠状动脉粥样硬化性心脏病患者血清miR-24-3p 和MARK4 水平表达与心功能的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年04期
页码:
10-15
栏目:
论著
出版日期:
2024-07-15

文章信息/Info

Title:
Study on the Correlation between Serum miR-24-3p and MARK4 Level Expressions and Cardiac Function in Patients with Coronary Atherosclerotic Heart Disease
文章编号:
1671-7414（2024）04-010-06
作者:
严 双1a; 杜 娟1b; 曹 露1c; 尹丽娟1d; 钱黎明2; 李其勇1a
（1. 四川省医学科学院∕四川省人民医院 a. 心血管内科；b. 老年心血管内科；c. 消化科；d. 肾脏科，成都610036；2. 四川大学华西第二医院产科，成都 610044）
Author(s):
YAN Shuang1a; DU Juan1b; CAO Lu1c; YIN Lijuan1d; QIAN Liming2; LI Qiyong1a
(1a. Department of Cardiovascular Medicine；1b. Department of Geriatric Cardiovascular Medicine；1c. Department of Gastroenterology；1d. Department of Nephrology，Sichuan Academy of Medical Sciences / Sichuan Provincial People’s Hospital, Chengdu 610036, China；2. Department of Obstetrics, West China Second University Hospital, Sichuan University, Chengdu 610044, China)
关键词:
冠状动脉粥样硬化性心脏病; 微小核糖核酸-24-3p; 微管亲和调节激酶4; 心功能
分类号:
R541.4；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.04.003
文献标志码:
A
摘要:
目的 探讨动脉粥样硬化性心脏病（coronary atherosclerotic heart disease，CHD）患者血清中微小RNA（microRNA，miR）-24-3p，微管亲和调节激酶4（microtubule affinity-regulating kinase 4，MARK4）水平与心功能的相关性。方法 选取2021 年8 月～ 2023 年4 月间在四川省人民医院就诊的138 例CHD 患者为研究对象（CHD 组），依据美国纽约心脏病学会（New York Heart Association，NYHA）分级，将CHD 组患者分为Ⅱ级（n=39），Ⅲ级（n=68）和Ⅳ级（n=31）。另选取同期健康体检人员123 例作为健康组。采用实时荧光定量PCR（qRT-PCR）法检测miR-24-3p 及MARK4 表达水平。检测CHD 组及健康组人员心功能指标。采用Pearson 法分析血清miR-24-3p，MARK4 及与心功能指标间的相关性。结果 与健康组比较，CHD 组患者的MARK4（1.19±0.21 vs 1.00±0.20），左心室收缩末期内径（LVESd）、左心室舒张末期内径（LVEDd）、室间隔厚度（IVS）、左室舒张末期容积（LVEDv）、左室收缩末期容积（LVESv）及左心室质量（LVmass）水平升高（t=7.462，18.242，23.888，9.941，2.812，12.520，11.029），miR-24-3p[（0.62±0.11）vs（1.00±0.17）]，LVEF 及心输出量（CO）水平降低（t=20.821，10.212，18.188），差异具有统计学意义（均P ＜ 0.05）。随着心功能分级加重，MARK4 表达水平（1.09±0.19，1.18±0.17，1.32±0.22），LVESd，LVEDd，IVS，LVEDv，LVESv 及LV mass 逐渐升高（F=13.025，10.606，11.920，57.956，29.680，21.253，12.954），miR-24-3p 表达水平（0.84±0.15，0.61±0.11，0.37±0.08），LVEF，CO 水平逐渐降低（F=139.227，9.720，13.411），差异具有统计学意义（均P ＜ 0.05）。Pearson 相关性分析显示，CHD 组患者血清中miR-24-3p 与MARK4 表达呈负相关（r=-0.540，P<0.05）。CHD 组患者血清miR-24-3p 相对表达量与LVESd，LVEDd，IVS，LVEDv，LVESv 及LV mass 呈负相关（r=-0.656，-0.636，-0.617，-0.576，-0.492，-0.687， 均P ＜ 0.05），与LVEF 及CO 呈正相关（r=0.570，0.683，均P ＜ 0.05）；MARK4 相对表达量与LVESd，LVEDd，IVS，LVEDv，LVESv 及LV mass 呈正相关（r=0.503，0.542，0.508，0.624，0.516，0.560，均P ＜ 0.05），与LVEF 及CO 呈负相关（r=-0.594，-0.525，均P ＜ 0.05）。结论 CHD 患者血清中miR-24-3p 表达降低，MARK4 表达升高，且二者与心功能指标间存在显著相关性。
Abstract:
Objective To investigate the correlation between serum microRNA (miR) -24-3p，microtubule affinity-regulating kinase 4 (MARK4) level expression and cardiac function in serum of patients with coronary atherosclerotic heart disease (CHD). Methods From August 2021 to April 2023, 138 CHD patients who visited Sichuan Provincial People’s Hospital were collected as the study subjects (CHD group). According to the New York Heart Association (NYHA) grading, patients in the CHD group were separated into grade II (n=39), grade III (n=68), and grade IV (n=31). Additionally, 123 individuals who underwent physical examinations were selected as the health group during the same period. Real-time fluorescence quantitative PCR (qRT-PCR) method was applied to detect the expression levels of miR-24-3p and MARK4. Cardiac function indicators in the CHD and health groups were detected. Pearson method was applied to analyze the correlation among serum miR-24-3p, MARK4, and cardiac function indicators. Results Compared with the healthy group, the levels of MARK4 (1.19±0.21 vs 1.00±0.20)，left ventricular end-systolic dimension (LVESd), left ventricular enddiastolic dimension (LVEDd), interventricular septal thickness (IVS), left ventricular end-diastolic volume (LVEDv), left ventricular end-systolic volume (LVESv), and left ventricular mass (LV mass) in the CHD group were increased（t=7.462，18.242，23.888，9.941，2.812，12.520，11.029）, while the levels of miR-24-3p (0.62±0.11 vs 1.00±0.18), left ventricular ejection fraction (LVEF), and cardiac output (CO) were reduced （t=20.821，10.212，18.188）, and the differences were statistically significant (all P<0.05). As cardiac function grading increased, MARK4 expression levels (1.09±0.19, 1.18±0.17, 1.32±0.22), LVESd, LVEDd, IVS, LVEDv, LVESv and LV mass were increased（F=13.025, 10.606, 11.920, 57.956, 29.680, 21.253, 12.954）, and miR-24-3p expression levels （0.84±0.15, 0.61±0.11, 0.37±0.08）, LVEF and CO were decreased（F=139.227, 9.720, 13.411), and the differences were statistically significant(all P<0.05). Pearson correlation analysis showed a negative correlation between miR-24-3p and MARK4 expression in the serum of patients with CHD (r=-0.540, P<0.05). The relative expression of miR-24-3p in the serum of patients with CHD was negatively correlated with LVESd, LVEDd, IVS, LVEDv, LVESv and LV mass (r=-0.656，-0.636，-0.617，-0.576，- 0.492，-0.687，all P<0.05)，but positively correlated with LVEF and CO (r=0.570, 0.683, all P<0.05). The relative expression level of MARK4 was positively correlated with LVESd, LVEDd, IVS, LVEDv, LVESv, and LV mass (r=0.503, 0.542, 0.508, 0.624, 0.516, 0.560, all P<0.05), but negatively correlated with LVEF and CO (r=-0.594, -0.525, all P<0.05). Conclusion The expression of miR-24-3p in the serum of CHD patients was decreased, while the expression of MARK4 was increased. There was an obvious correlation between the two and cardiac function indicators.

参考文献/References:

[1] 关小明, 陈锴, 王文会. 冠状动脉粥样硬化性心脏病患者血清骨形态发生蛋白4, N1- 甲基烟酰胺和血管生成素样蛋白8 水平检测的临床意义[J]. 现代检验医学杂志, 2021, 36(4): 15-20. GUAN Xiaoming, CHEN Kai, WANG Wenhui. Clinical significance of detection of serum bone morphogenetic protein 4, N1-methylnicotinamide and angiopoietin-like protein 8 levels in patients with coronary atherosclerotic heart disease[J]. Journal of Modern Laboratory Medicine, 2021, 36(4): 15-20.
[2] 黄文军, 张靖, 马勇翔, 等. 老年冠心病患者动脉硬化相关参数与心脏结构和功能的相关性研究[J]. 河北医药, 2021, 43(21): 3304-3306. HUANG Wenjun, ZHANG Jing, MA Yongxiang, et al. Study on the correlation between parameters of pulse wave conduction velocity and cardiac structure and function in patients with coronary heart disease[J]. Hebei Medical Journal, 2021, 43(21): 3304-3306.
[3] 褚福永, 刘巍, 刘红旭. 益气逐瘀方对急性心肌梗死大鼠miR-24 表达及心肌细胞凋亡的影响[J]. 世界中医药, 2016, 11(3): 392-394. CHU Fuyong, LIU Wei, LIU Hongxu. Effects of Shen Yuan Dan on miR-24 expression and myocardium cell apoptosis in myocardial infarction rats[J]. World Chinese Medicine, 2016, 11(3): 392-394.
[4] VOURA M, ANWAR S, SIGALA I, et al. Synthesis, structural modification, and bioactivity evaluation of substituted acridones as potent microtubule Affinity-Regulating kinase 4 inhibitors[J]. ACS Pharmacology & Translational Science, 2023, 6(7): 1052-1074.
[5] 于莉莉, 尚莎莎, 周阳, 等. 微管亲和调节激酶4 的生理功能及其在心血管疾病中的研究进展[J]. 解放军医学院学报, 2022, 43(5): 613-617. YU Lili, SHANG Shasha, ZHOU Yang, et al. Research advances in physiological function of MARK4 and its role in cardiovascular diseases[J]. Academic Journal of Chinese PLA Medical School, 2022, 43(5): 613-617.
[6] CLEMENT M, CHEN Xiao, CHENOWETH H L, et al. MARK4 (microtubule affinity-regulating kinase 4)-dependent inflammasome activation promotes atherosclerosis-brief report[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2019, 39(8): 1645-1651.
[7] 许秋雯, 郭自强. 慢性心力衰竭心功能评价方法现状分析[J]. 中国医药导报, 2020, 17(10): 35-37, 49. XU Qiuwen, GUO Ziqiang. Status analysis of function evaluate methods in chronic heart failure[J]. China Medical Herald, 2020, 17(10): 35-37, 49.
[8] 中华医学会心血管病学分会心力衰竭学组, 中国医师协会心力衰竭专业委员会, 中华心血管病杂志编辑委员会. 中国心力衰竭诊断和治疗指南2018[J].中华心血管病杂志, 2018, 46(10): 760-789. Heart Failure Group of Chinese Society of Cardiology of Chinese Medical Association, Chinese Heart Failure Association of Chinese Medical Doctor Association, Editorial Board of Chinese Journal of Cardiology. Chinese guidelines for the diagnosis and treatment of heart failure 2018[J]. Chinese Journal of Cardiology, 2018, 46(10): 760-789.
[9] 张芙成, 苏淑红, 常丹阳, 等. IL-36 在冠状动脉粥样硬化性心脏病患者中的表达及其对CD8+T 细胞的功能调控[J]. 中华微生物学和免疫学杂志, 2023,43(6): 464-472. ZHANG Fucheng, SU Shuhong, CHANG Danyang, et al. Expression of IL-36 and its regulatory effects on CD8+ T cell function in patients with coronary atherosclerotic heart disease[J]. Chinese Journal of Microbiology and Immunology, 2023, 43(6): 464-472.
[10] 刘昌燕, 严其高, 徐刚锋, 等. 冠状动脉粥样硬化性心脏病经皮冠状动脉介入治疗术后患者出血事件发生风险模型及验证[J]. 中国药业, 2023, 32(11): 81-85. LIU Changyan, YAN Qigao, XU Gangfeng, et al. Risk model and validation of bleeding events in patients with coronary heart disease underwent percutaneous coronary intervention[J]. China Pharmaceuticals, 2023, 32(11): 81-85.
[11] RIAD M. Association of helicobacter pylori infection with coronary artery disease: is it an Independent risk factor?[J]. The Egyptian Heart Journal, 2021, 73(1): 61.
[12] YAO Tianci, LU Weilin, KE Jinshan, et al. Residual risk of coronary atherosclerotic heart disease and severity of coronary atherosclerosis assessed by ApoB and LDL-C in participants with statin treatment: a retrospective cohort study[J]. Frontiers in Endocrinology, 2022, 13: 865863.
[13] 李艳, 于永丽, 董子豪, 等. 同型半胱氨酸调控miRNA在心血管疾病中的作用研究进展[J]. 中国动脉硬化杂志, 2023, 31(8): 725-730. LI Yan, YU Yongli, DONG Zihao, et al. Research progress on the role of homocysteine regulated miRNA in cardiovascular diseases[J]. Chinese Journal of Arteriosolerosis, 2023, 31(8)： 725-730.
[14] RASO A, DIRKX E, PHILIPPEN L E, et al. Therapeutic delivery of miR-148a suppresses ventricular dilation in heart failure[J]. Molecular Therapy, 2019, 27(3): 584-599.
[15] TAN Hong, QI Jie, FAN Boyuan, et al. MicroRNA-24-3p attenuates myocardial ischemia/reperfusion injury by suppressing RIPK1 expression in mice[J]. Cellular Physiology and Biochemistry, 2018, 51(1): 46-62.
[16] KAZEMIAN S, AHMADI R, FERNS G A, et al. Correlation of miR-24-3p and miR-595 expression with CCL3, CCL4, IL1-beta, TNFalphaIP3, and NF-kappaBIalpha genes in PBMCs of patients with coronary artery disease[J]. EXCLI Journal, 2022, 21: 1184-1195.
[17] NAQVI A A T, JAIRAJPURI D S, HUSSAIN A, et al. Impact of glioblastoma multiforme associated mutations on the structure and function of MAP/microtubule affinity regulating kinase 4[J]. Journal of Biomolecular Structure & Dynamics, 2021, 39(5): 1781-1794.
[18] YANG Kun, CAI Jiarui, PAN Miao, et al. Mark4 inhibited the browning of white adipose tissue by promoting adipocytes autophagy in mice[J]. International Journal of Molecular Sciences, 2020, 21(8): 2752.
[19] TIAN Liang, LIU Guangfan, KANG Ziqi, et al. Microtubule affinity-regulating kinase 4 promotes oxidative stress and mitochondrial dysfunction by activating NF-κB and inhibiting AMPK pathways in porcine placental trophoblasts[J]. Biomedicines, 2022, 10(1): 165.
[20] SUN Jiadong, LIAO Wenting, SU Kai, et al. Suberoylanilide hydroxamic acid attenuates interleukin-1β-induced interleukin-6 upregulation by inhibiting the microtubule affinity-regulating kinase 4/nuclear factor-κb pathway in synovium-derived mesenchymal stem cells from the temporomandibular joint[J]. Inflammation, 2020, 43(4): 1246-1258.
[21] ALAM M, AHMED S, ABID M, et al. Therapeutic targeting of microtubule affinity-regulating kinase 4 in cancer and neurodegenerative diseases[J]. Journal of Cellular Biochemistry, 2023, 124(9): 1223-1240.
[22] WANG Lulu, PU Wenchen, WANG Chun, et al. Microtubule affinity regulating kinase 4 promoted activation of the NLRP3 inflammasome-mediated pyroptosis in periodontitis[J]. Journal of Oral Microbiology, 2022, 14(1): 2015130.

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备注/Memo

备注/Memo:
作者简介：严双（1986-），女，本科，中级，研究方向：心血管疾病，E-mail：e77gi4@163.com。
更新日期/Last Update: 2024-07-15