[1]李继恩,张 静,付大清,等.老年膝关节骨性关节炎患者血清LncRNA BLACAT1 和LncRNA-H19 水平表达与病情严重程度的相关性研究[J].现代检验医学杂志,2024,39(04):34-39.[doi:10.3969/j.issn.1671-7414.2024.04.007]
　LI Jien,ZHANG Jing,FU Daqing,et al.Study on Correlation between the Expression of Serum LncRNA BLACAT1 and LncRNA-H19 Levels and Severity of Elderly Knee Osteoarthritis[J].Journal of Modern Laboratory Medicine,2024,39(04):34-39.[doi:10.3969/j.issn.1671-7414.2024.04.007]
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老年膝关节骨性关节炎患者血清LncRNA BLACAT1 和LncRNA-H19 水平表达与病情严重程度的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年04期
页码:
34-39
栏目:
论著
出版日期:
2024-07-15

文章信息/Info

Title:
Study on Correlation between the Expression of Serum LncRNA BLACAT1 and LncRNA-H19 Levels and Severity of Elderly Knee Osteoarthritis
文章编号:
1671-7414（2024）04-034-06
作者:
李继恩1; 张 静2; 付大清1; 张 洋1
（1. 荆门市中医医院推拿科，湖北荆门 448000；2. 荆门市中医医院热电厂分院综合科，湖北荆门 448000）
Author(s):
LI Jien1; ZHANG Jing2; FU Daqing1; ZHANG Yang1
（1. Department of Massage，Jingmen Traditional Chinese Medicine Hospital, Hubei Jingmen 448000, China；2. Department of General，Thermal Power Plant Branch of Jingmen Traditional Chinese Medicine Hospital, Hubei Jingmen 448000, China）
关键词:
长链非编码RNA 膀胱癌相关转录因子1; 长链非编码RNA -H19; 老年膝关节骨性关节炎
分类号:
R684.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.04.007
文献标志码:
A
摘要:
目的 探究老年膝关节骨性关节炎（knee osteoarthritis，KOA）患者血清长链非编码RNA(long non-codingRNA，LncRNA) 膀胱癌相关转录因子1(bladder cancer associated transcript 1，BLACAT1) 和长链非编码RNA(long noncodingRNA，LncRNA)-H19 水平表达与病情严重程度的相关性。方法 收集2021 年11 月～ 2023 年2 月在荆门市中医医院进行诊治的120 例老年KOA 患者作为研究对象（KOA 组）。根据凯尔格伦- 劳伦斯（Kellgren-Lawrence，K-L）分级将KOA 组患者分为轻度组（n=41）、中度组（n=45）和重度组（n=34），另以同期行健康检查者为对照组（n=120）。实时荧光定量PCR 法测定血清LncRNA BLACAT1 和LncRNA-H19 水平，比较KOA 组患者与对照组血清LncRNA BLACAT1 和LncRNA-H19 水平，分析不同病情程度KOA 组患者血清LncRNA BLACAT1 和LncRNA-H19 水平。Spearman 相关性分析KOA 患者血清LncRNA BLACAT1 和LncRNA-H19 水平与病情程度间的关系，受试者工作特征（receiver operating characteristic，ROC）曲线分析血清LncRNA BLACAT1 和LncRNA-H19 水平对KOA 的诊断价值。结果 KOA 组患者血清LncRNA BLACAT1（1.31±0.31），LncRNA-H19（1.41±0.37）水平均显著高于健康对照组（0.99±0.24，1.03±0.25），差异具有统计学意义（t=8.941，9.322，均P ＜ 0.05）；重度组KOA 患者血清LncRNABLACAT1（1.53±0.36），LncRNA-H19（1.71±0.44）水平均显著高于轻度组（1.13±0.27，1.18±0.29）和中度组（1.32±0.31，1.40±0.38）（q =7.806，4.183；8.709，5.200，均P ＜ 0.05），且中度组高于轻度组（q =3.983，3.884，均P ＜ 0.05），差异具有统计学意义。Spearman 相关性分析结果显示，血清LncRNA BLACAT1，LncRNA-H19 水平与病情程度均呈显著正相关（r=0.552，0.414，均P ＜ 0.05）。ROC 曲线结果显示，LncRNA BLACAT1，LncRNA-H19 单独诊断KOA 的曲线下面积（area under the curve，AUC）分别为0.860，0.869，特异度分别为74.2%，80.8%，敏感度分别为66.7%，69.1%，两者联合诊断KOA 的AUC 为0.966，敏感度和特异度分别为90.0%，81.7%。结论 KOA 患者血清LncRNABLACAT1 和LncRNA-H19 水平均显著升高，且与KOA 患者病情程度呈显著正相关，两者联合检测可有效预测KOA的发生。
Abstract:
Objective To explore the correlation between the expression of serum long non-coding RNA bladder cancer associated transcript 1 (LncRNA BLACAT1) and long non-coding RNA(LncRNA)-H19 levels and the severity of elderly knee osteoarthritis (KOA). Methods A total of 120 elderly KOA patients who underwent diagnosis and treatment in Jingmen Hospital of Traditional Chinese Medicine from November 2021 to February 2023 were selected as subjects (KOA group). Based on the Kelgren-Lawrence (K-L) classification, these KOA group patients were grouped into mild group (n=41), moderate group (n=45), and severe group (n=34). Meanwhile, those who underwent health examinations at the same time were regarded as control group (n=120). Real-time fluorescence quantitative PCR method was applied to measure the levels of serum LncRNA BLACAT1 and LncRNA-H19. The serum levels of LncRNA BLACAT1 and LncRNA-H19 were compared between KOA group and the control group. The serum levels of LncRNA BLACAT1 and LncRNA-H19 in KOA patients with different degrees of illness were analyzed. Spearman correlation was applied to analyze the relationship between the expression of serum LncRNA BLACAT1, LncRNA-H19 levels and the severity of KOA patients. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of serum LncRNA BLACAT1 and LncRNA-H19 levels for KOA. Results The serum levels of LncRNA BLACAT1 (1.31±0.31) and LncRNA-H19 (1.41±0.37) in KOA patients were higher than those in the healthy control group (0.99±0.24, 1.03±0.25), and the differences were statistically significant (t=8.941, 9.322, all P<0.05). The serum levels of LncRNA BLACAT1 (1.53±0.36) and LncRNA-H19 (1.71±0.44) in the severe group were higher than those in the mild (1.13±0.27, 1.18±0.29) and moderate groups (1.32±0.31, 1.40±0.38) (q =7.806, 4.183；8.709, 5.200), and the serum levels of the moderate group were higher than that of the mild group(q=3.983, 3.884), and the differences were statistically significant , respectively(all P<0.05). Spearman correlation analysis results showed that the serum levels of LncRNA BLACAT1 and LncRNA-H19 were positively correlated with the severity of the disease (r=0.552, 0.414, all P<0.05). The results of ROC showed that the AUCs of LncRNA BLACAT1 and LncRNA-H19 alone diagnosed KOA were 0.860 and 0.869, with specificity of 74.2% and 80.8%, and sensitivity of 66.7% and 69.1%, respectively. The AUC of the combined diagnosis of KOA was 0.966, with a sensitivity and a specificity of 90.0%, 81.7%, respectively. Conclusion The serum levels of LncRNA BLACAT1 and LncRNA-H19 in KOA patients were elevated and were positively correlated with the severity of KOA patients. Combined detection of the two could effectively predict the occurrence of KOA.

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备注/Memo

备注/Memo:
基金项目：荆门市一般科技计划项目（2022YFYB125）。
作者简介：李继恩（1982-），男，本科，副主任医师，研究方向：脊柱关节病诊治， E-mail：ji4n4v@163.com。
通讯作者：张静（1981-），女，本科，副主任医师，研究方向：临床及医学统计学分析， E-mail：annabel111@163.com。
更新日期/Last Update: 2024-07-15