[1]付超英,黄士月,张云霞.直肠癌组织中LncRNA TTN-AS1 及角鲨烯环氧酶表达与临床病理特征及预后相关性研究[J].现代检验医学杂志,2024,39(05):12-16+53.[doi:10.3969/j.issn.1671-7414.2024.05.003]
　FU Chaoying,HUANG Shiyue,ZHANG Yunxia.Correlation between the Expression of LncRNA TTN-AS1 and Squalene Epoxidase in Rectal Cancer Tissue and Clinicopathological Characteristics and Prognosis[J].Journal of Modern Laboratory Medicine,2024,39(05):12-16+53.[doi:10.3969/j.issn.1671-7414.2024.05.003]
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直肠癌组织中LncRNA TTN-AS1 及角鲨烯环氧酶表达与临床病理特征及预后相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年05期
页码:
12-16+53
栏目:
论著
出版日期:
2024-09-15

文章信息/Info

Title:
Correlation between the Expression of LncRNA TTN-AS1 and Squalene Epoxidase in Rectal Cancer Tissue and Clinicopathological Characteristics and Prognosis
文章编号:
1671-7414（2024）05-012-06
作者:
付超英1; 黄士月2; 张云霞1
（1. 成都中医药大学附属医院龙泉医院病理科，成都 610100；2. 成都市新都区人民医院病理科，成都 610500）
Author(s):
FU Chaoying1; HUANG Shiyue2; ZHANG Yunxia1
（1.Department of Pathology, Longquan Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610100, China；2.Department of Pathology, Chengdu Xindu District People’s Hospital，Chengdu 610500, China）
关键词:
直肠癌; 长链非编码RNA TTN-AS1; 角鲨烯环氧酶; 临床病理特征
分类号:
R735.37；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.05.003
文献标志码:
A
摘要:
目的 研究直肠癌组织中长链非编码RNA（long non-coding RNA，LncRNA）TTN-AS1 和角鲨烯环氧酶(squalene epoxidase，SQLE）的表达与临床病理特征及预后相关性。方法 选取2018 年1 月～ 2020 年1 月成都中医药大学附属医院诊治的90 例直肠癌患者为研究对象。采用荧光定量PCR 检测组织LncRNA TTN-AS1 表达。采用免疫组织化学法检测组织SQLE 表达。分析LncTTN-AS1，SQLE 与直肠癌临床病理特征的关系。KM 曲线分析LncRNA TTNAS1，SQLE 对直肠癌预后的影响。COX 回归分析影响直肠癌预后的因素。结果 直肠癌组织中LncRNA TTN-AS1（3.12±0.45）相对表达量，SQLE（71.11%）蛋白阳性率均高于癌旁组织（0.91±0.12，8.89%），差异具有统计学意义（t/χ2=45.156，72.593，均P ＜ 0.001）。直肠癌组织中LncRNA TTN-AS1 与SQLE 表达呈正相关（r=0.589，P ＜ 0.001）。TNM 分期Ⅲ期、淋巴结转移癌组织中Lnc RNA TTN-AS1 相对表达量（4.26±0.52，4.10±0.49 ）、SQLE（88.57%，91.43%）阳性率高于TNM 分期Ⅰ～Ⅱ期（2.39±0.40，60.00%）、无淋巴结转移癌组织（2.50±0.42，58.18%），差异具有统计学意义（t/χ2=8.409 ～ 19.211，均 P<0.05）。LncRNA TTN-AS1 高表达组和低表达组三年生存率分别为50.00%（22/44）和86.96%（40/46），差异具有统计学意义（Log-rank χ2=14.205，P=0.001）。SQLE 阳性组和阴性组三年生存率分别为64.06%（41/64）和88.46%（23/26），差异具有统计学意义（Log-rank χ2=6.291，P=0.012）。LncRNATTNAS1高表达（HR=2.552，P=0.001）、SQLE 阳性（HR=1.754，P=0.004）、TNM 分期Ⅲ期（HR=2.797，P=0.011）和淋巴结转移（HR=1.635，P=0.03）是直肠癌预后的独立危险因素。结论 直肠癌组织中LncRNA TTN-AS1，SQLE 表达升高，与直肠癌肿瘤TNM 分期及淋巴结转移有关，是评估直肠癌预后的肿瘤标志物。
Abstract:
Objective To study the expression of long non-coding RNA(LncRNA TTN-AS1)TTN-AS1 and squalene epoxidase (SQLE) in rectal cancer, and their correlation with clinicopathological characteristics and prognosis. Methods A total of 90 rectal cancer patients diagnosed and treated in Longquan Hospital of Chengdu University of Traditional Chinese Medicine from January 2018 to January 2020 were selected as the research subjects. Fluorescence quantitative PCR was used to detect the expression of LncRNA TTN-AS1 in tissues. Immunohistochemistry was used to detect the expression of SQLE in tissues. The relationship between LncRNA TTN-AS1, SQLE and clinicopathological characteristics of rectal cancer were compared. K-M curve analysis was used to analyze the impact of LncRNA TTN-AS1 and SQLE on the prognosis of rectal cancer. COX regression analysis was used to analyze factors affecting the prognosis of rectal cancer. Results The relative expression level of Lnc RNA TTN-AS1 (3.12 ± 0.45) and the positivity rate of SQLE (71.11%) protein in rectal cancer tissues were higher than those in adjacent tissues（0.91±0.12，8.89%）, and the differences were significant (t/χ2=45.156, 72.593, all P<0.001). The expression of Lnc RNA TTN-AS1 was positively correlated with SQLE in rectal cancer tissue (r=0.589, P ＜ 0.001). The relative expressions of Lnc RNA TTN-AS1 (4.26±0.52，4.10±0.49), SQLE (88.57%，91.43%) in tumor TNM stage III and lymph node metastasis were higher than those in tumor TNM stage I ～ II（2.39±0.40，60.00%） and tissues without lymph node metastasis（2.50±0.42，58.18%）, and the differences were significant（t/χ2=8.409 ～ 19.211, all P<0.05）. The 3-year survival rates of the LncTTN-AS1 high expression group and low expression group were 50.00% (22/44) and 86.96% (40/46), respectively, and the difference between the curves was significant (Log-rank χ2=14.205, P=0.001). The 3-year survival rates of the SQLE positive and negative groups were 64.06% (41/64) and 88.46% (23/26), respectively, and the difference between the curves was significant (Log-rank χ2=6.291, P=0.012). Lnc RNA TTN-AS1 high expression (HR=2.552, P=0.001), SQLE positive (HR=1.754, P=0.004), tumor TNM stage III(HR=1.625, P=0.03), and lymph node metastasis were (HR=2.797, P=0.011)independent risk factors for the prognosis of rectal cancer. Conclusion The increased expression of LncRNA TTN-AS1 and SQLE in rectal cancer tissue are associated with TNM staging and lymph node metastasis, and both are tumor marker for evaluating the prognosis of rectal cancer.

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备注/Memo

备注/Memo:
基金项目：四川省医学会（青年创新）科研课题（N0:Q20038）。
作者简介：付超英（1981-），女，硕士，主治医师，研究方向：直肠癌病理，E-mail：18982104242@163.com。
更新日期/Last Update: 2024-09-15