[1]高其亮a,毛星荐b,吴 钢a.急性胰腺炎患者血清miR-455-3p，miR-141-3p 水平表达与病情严重程度的关系研究[J].现代检验医学杂志,2024,39(05):41-46.[doi:10.3969/j.issn.1671-7414.2024.05.008]
　GAO Qilianga,MAO Xingjianb,WU Ganga.Study on the Relationship between the Expression of Serum miR-455-3p and miR-141-3p Levels and the Severity of Disease in Patients with Acute Pancreatitis[J].Journal of Modern Laboratory Medicine,2024,39(05):41-46.[doi:10.3969/j.issn.1671-7414.2024.05.008]
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急性胰腺炎患者血清miR-455-3p，miR-141-3p 水平表达与病情严重程度的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年05期
页码:
41-46
栏目:
论著
出版日期:
2024-09-15

文章信息/Info

Title:
Study on the Relationship between the Expression of Serum miR-455-3p and miR-141-3p Levels and the Severity of Disease in Patients with Acute Pancreatitis
文章编号:
1671-7414（2024）05-041-06
作者:
高其亮a; 毛星荐b; 吴 钢a
（都江堰市人民医院 a. 消化内科；b. 检验科，四川都江堰 611830）
Author(s):
GAO Qilianga;  MAO Xingjianb;  WU Ganga
（a.Department of Gastroenterology; b.Department of Laboratory, the People’s Hospital of Dujiangyan, Sichuan Dujiangyan 611830, China）
关键词:
急性胰腺炎; miRNA-455-3p; miRNA-141-3p
分类号:
R576；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.05.008
文献标志码:
A
摘要:
目的 探讨急性胰腺炎（acute pancreatitis，AP）患者血清miR-455-3p，miR-141-3p 水平表达与病情严重程度的关系。方法 选取2021 年11 月～ 2023 年11 月于都江堰市人民医院就诊的AP 患者133 例（AP 组）作为研究对象；根据病情程度将AP 患者分为重症组（n=43）和轻症组（n=90）。同期选取于该院体检健康者135 例作为对照组。实时荧光定量PCR（RT-qRCR）法检测血清miR-455-3p 和miR-141-3p 水平。Spearman 相关性分析血清miR-455-3p，miR-141-3p 水平与急性生理与慢性健康评分II（acute physiology and chronic health evaluation II，APACHE II）、Ranson 评分的相关性；受试者工作特征（ROC）曲线分析血清miR-455-3p，miR-141-3p 水平对重症AP 的诊断价值；多因素Logistics 回归分析影响重症AP 的发生因素。结果 AP 组血清miR-455-3p（0.61±0.13）低于对照组（1.12±0.21），miR-141-3p 水平（1.37±0.11）高于对照组（1.04±0.15），差异具有统计学意义（t=23.863，20.513，均P＜0.05）。重症组患者血清miR-455-3p水平（0.53±0.10）低于轻症组（0.64±0.12），miR-141-3p 水平（1.51±0.14）高于轻症组（1.30±0.15），差异具有统计学意义（t=6.056，7.713，均P<0.05）。重症组患者血清淀粉酶（serum amylase，AMS）、脂肪酶（lipase，LPS）水平及APACHEII，Ranson评分高于轻症组，差异具有统计学意义（t=2.227，2.290，18.267，11.259，均P ＜ 0.05）。Spearman 相关性分析结果表明，AP 患者血清miR-455-3p 与APACHEII，Ranson 评分呈负相关（r=-0.702，-0.783，均P ＜ 0.05），miR-141-3p 与APACHEII，Ranson 评分呈正相关（r=0.787，0.734，均P ＜ 0.05）。ROC 曲线结果表明血清miR-455-3p，miR-141-3p 水平单独及联合诊断重症AP的AUC分别为0.848，0.822和0.919，且二者联合诊断优于单独诊断（Z=3.081，2.524，均P＜0.05）。多因素Logistic 回归分析结果显示miR-455-3p 是影响重症AP的保护因素，miR-141-3p 是影响重症AP的危险因素（P<0.05）。结论 AP 患者血清miR-455-3p 下降和miR-141-3p 升高与病情严重程度密切相关。
Abstract:
Objective To investigate the relationship between the expression of miR-455-3p and miR-141-3p levels in serum and the severity of acute pancreatitis (AP) in patients. Methods A total of 133 AP patients (AP group) who visited the People’s Hospital of Dujiangyan from November 2021 to November 2023 were regarded as the study subjects. According to the severity of disease, AP patients were separated into a severe group（n=43） and a mild group（n=90）. During the same period，135 healthy individuals who underwent physical examination in the hospital were regarded as the control group. Real-time fluorescence quantitative PCR method was applied to detect serum levels of miR-455-3p and miR-141-3p. Spearman correlation analysis was applied to analyze the correlation between serum miR-455-3p and miR-141-3p levels and acute physiology and chronic health evaluation II（APACHE II）and Ranson scores. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of serum miR-455-3p and miR-141-3p levels for severe AP. Multivariate logistic regression was applied to analyze the factors affecting the occurrence of severe AP. Results The serum miR-455-3p level (0.61±0.13) in the AP group was lower than that in the control group (1.12±0.21), while the miR-141-3p level (1.37±0.11) was higher than that in the control group (1.04±0.15), with significant differences (t=23.863，20.513，all P<0.05). The serum miR-455-3p level (0.53±0.10) in the severe group was lower than that in the mild group (0.64±0.12), while the miR-141-3p level(1.51±0.14) was higher than that in the mild group (1.30±0.15), with significant differences (t=6.056，7.713，all P<0.05). The serum amylase（AMS），lipase（LPS）levels，APACHE II and Ranson scores in the severe group were higher than those in the mild group ，and the differences were significant(t=2.227, 2.290, 18.267, 11.259, all P<0.05). Spearman correlation analysis results showed that the serum miR-455-3p of AP patients was negatively correlated with APACHEII and Ranson scores (r=- 0.702, -0.783, all P<0.05), while miR-141-3p was positively correlated with APACHEII and Ranson scores (r=0.787, 0.734, all P<0.05). ROC curve results showed that the AUCs of serum miR-455-3p and miR-141-3p levels alone and combination of severe AP were 0.848，0.822 and 0.919, respectively. And the combination detection was superior to single detection (Z=3.081, 2.524, all P<0.05). Multivariate Logistic regression analysis showed that miR-455-3p was a protective factor affecting severe AP, while miR-141-3p was a risk factor affecting severe AP (P<0.05). Conclusion The decrease of serum miR-455-3p and the increase of miR-141-3p in AP patients are closely related to the severity of disease.

参考文献/References:

[1] 陈颖, 王伟佳, 胡婷, 等. 急性胰腺炎患者血清C1q补体水平与疾病严重程度的相关性研究[J]. 现代检验医学杂志, 2021, 36(4): 45-50. CHEN Ying, WANG Weijia, HU Ting, et al. Research on relevant serum C1q complement level in the severity of acute pancreatitis[J]. Journal of Modern Laboratory Medicine, 2021, 36(4): 45-50.
[2] 杨威, 黄咏宁, 吕元博, 等. 重症急性胰腺炎患者外周血TLR4，TRAF6 的表达及与并发肝损伤的关系[J]. 中国现代医学杂志, 2023, 33(12): 18-23. YANG Wei, HUANG Yongning, L? Yuanbo, et al. Expressions of TLR4 and TRAF6 in the peripheral blood of patients with severe acute pancreatitis and their associations with liver injury[J]. China Journal of Modern Medicine, 2023, 33(12): 18-23.
[3] 吴冲, 邢益门, 周婷, 等. 老年急性胰腺炎患者血清miR-29a 表达水平及其对并发急性肾损伤的预测价值[J]. 中国老年学杂志, 2023, 43(18): 4392-4394. WU Chong, XING Yimen, ZHOU Ting, et al. Serum miR-29a expression level in elderly patients with acute pancreatitis and its predictive value for concurrent acute kidney injury[J]. Chinese Journal of Gerontology, 2023, 43(18): 4392-4394.
[4] 刘兴涛, 王天舒, 覃海森, 等. 急性胰腺炎患者外周血miRNA-16 和miRNA-192 表达及其与病情严重程度的相关性[J]. 岭南急诊医学杂志, 2021, 26(4):348-351. LIU Xingtao, WANG Tianshu, QIN Haisen, et al. Expression of miRNA-16 and miRNA-192 in peripheral blood of patients with acute pancreatitis and its correlation with disease severity[J]. Lingnan Journal of Emergency Medicine, 2021, 26(4): 348-351.
[5] HAO Shunxin, YAO Zhi, LIU Yifeng. Hsa_ circ_0000106 acts as a tumor promoter in pancreatic cancer by targeting the miR-455-3p/HDAC4[J]. Hormone and Metabolic Research, 2023, 55(10): 722- 732.
[6] HU Qiongyi, GONG Wen, GU Jieyu, et al. Plasma microRNA profiles as a potential biomarker in differentiating adult-onset still’s disease from sepsis[J]. Frontiers in Immunology, 2018, 9: 3099.
[7] 中国医师协会胰腺病学专业委员会. 中国急性胰腺炎多学科诊治共识意见[J]. 临床肝胆病杂志, 2015,31(11): 1770-1775. Pancreatology Committee of Chinese Medical Doctor Association. Chinese consensus on the multidisciplinary treatment (MDT) of acute pancreatitis[J]. Journal of Clinical Hepatology, 2015, 31(11): 1770-1775.
[8] 中华医学会外科学分会胰腺外科学组. 中国急性胰腺炎诊治指南(2021）[J]. 中华消化外科杂志, 2021,20(7): 730-739. Chinese Pancreatic Surgery Association, Chinese Society of Surgery, Chinese Medical Association. Guidelines for diagnosis and treatment of acute pancreatitis in China (2021)[J]. Chinese Journal of Digestive Surgery, 2021, 20(7): 730-739.
[9] 李佳敏, 房智超, 王树楷, 等. 线粒体功能障碍及线粒体自噬异常在急性胰腺炎中的作用[J]. 中国现代医学杂志, 2023, 33(12): 58-64. LI Jiamin, FANG Zhichao, WANG Shukai, et al. The role of mitochondrial dysfunction and abnormal mitophagy in acute pancreatitis[J]. China Journal of Modern Medicine, 2023, 33(12): 58-64.
[10] 邓雍, 马涛, 张宁. 重症急性胰腺炎病人外周血微RNA-143-3p， 环氧合酶-2 表达水平与辅助性T细胞17/ 调节性T 细胞平衡的关系[J]. 安徽医药,2023, 27(11): 2198-2202. DENG Yong, MA Tao, ZHANG Ning. The relationship between the expression levels of miR-143-3p, COX-2 and Th17/Treg balance in peripheral blood of patients with severe acute pancreatitis[J]. Anhui Medical and Pharmaceutical Journal, 2023, 27(11): 2198-2202.
[11] 杨卿青, 秦继武, 张超, 等. 血清抗凝血酶- Ⅲ联合改良CT 严重指数评估急性胰腺炎患者病情严重程度及预后的临床价值[J]. 临床和实验医学杂志,2023, 22(17): 1797-1800. YANG Qingqing, QIN Jiwu, ZHANG Chao, et al. Value of serum antithrombin- Ⅲ combined with modified CT severity index in evaluating the severity and prognosis of acute pancreatitis patients[J]. Journal of Clinical and Experimental Medicine, 2023, 22(17): 1797-1800.
[12] YANG Yi, HUANG Qilin, LUO Chen, et al. MicroRNAs in acute pancreatitis: from pathogenesis to novel diagnosis and therapy[J]. Journal of Cellular Physiology, 2020, 235(3): 1948-1961.
[13] 陈映群, 张卫星, 尹子涵, 等. 微小RNA-375 在急性胰腺炎患者外周血中的表达及与并发急性肝损伤的相关性[J]. 分子诊断与治疗杂志, 2022, 14(12):2150-2153, 2158. CHEN Yingqun, ZHANG Weixing, YIN Zihan, et al. Expression of microRNA-375 in peripheral blood of patients with acute pancreatitis and its correlation with concurrent acute liver injury[J]. Journal of Molecular Diagnosis and Therapy, 2022, 14(12): 2150-2153, 2158.
[14] VASU S, SARACINO G, DARDEN C M, et al. Clinical and biological significance of circulating miRNAs in chronic pancreatitis patients undergoing total pancreatectomy with islet autotransplantation[J]. Clinical and Translational Medicine, 2023, 13(10): e1434.
[15] ZHOU Wence, DONG Shi, CHEN Zhou, et al. New challenges for microRNAs in acute pancreatitis: progress and treatment[J]. Journal of Translational Medicine, 2022, 20(1): 192.
[16] KURASHIGE S, MATSUTANI N, AOKI T, et al. Evaluation of circulating miR-216a and miR-217 as biomarkers of pancreatic damage in the L-arginineinduced acute pancreatitis mouse model[J]. Journal of Toxicological Sciences, 2023, 48(10): 527-534.
[17] ZHAN Ting, ZHU Qingxi, HAN Zheng, et al. MiR- 455-3p functions as a tumor suppressor by restraining Wnt/β-catenin signaling via TAZ in pancreatic cancer[J]. Cancer Management and Research, 2020, 12: 1483-1492.
[18] ZHAN Yinchu, CHEN Chenlin, WU Zhiqiang, et al. MiR-455-3p ameliorates pancreatic acinar cell injury by targeting SLC2A1[J]. Peer J, 2023, 11: e15612.
[19] YU Yali, DONG Guixiang, LI Zijun, et al. Circ-LRP6 contributes to osteosarcoma progression by regulating the miR-141-3p/HDAC4/HMGB1 axis[J]. International Journal of Oncology, 2022, 60(4): 38.
[20] SONG Guodong, ZHOU Jia, SONG Ruimei, et al. Long noncoding RNA H19 regulates the therapeutic efficacy of mesenchymal stem cells in rats with severe acute pancreatitis by sponging miR-138-5p and miR-141-3p[J]. Stem Cell Research & Therapy, 2020, 11(1): 420.

相似文献/References:

[1]陈　颖,王伟佳,胡　婷,等.急性胰腺炎患者血清C1q补体水平与疾病严重程度的相关性研究[J].现代检验医学杂志,2021,36(04):45.[doi:10.3969/j.issn.1671-7414.2021.04.010]
　CHEN Ying,WANG Wei-jia,HU Ting,et al.Research on Relevant Serum C1q Complement Level inthe Severity of Acute Pancreatitis[J].Journal of Modern Laboratory Medicine,2021,36(05):45.[doi:10.3969/j.issn.1671-7414.2021.04.010]

备注/Memo

备注/Memo:
基金项目：四川省卫生健康委员会科研课题(19PJ027)。
作者简介：高其亮（1987-），男，本科，主治医师，研究方向：胆胰疾病、肝炎、胰腺炎，E-mail：gant062446@163.com。
更新日期/Last Update: 2024-09-15