[1]熊小芹a,冯潇宇a,毛爱珺a,等.2 型糖尿病患者血清hsa-miR-30c-5p 水平表达对微血管并发症的预测价值研究[J].现代检验医学杂志,2024,39(05):63-68.[doi:10.3969/j.issn.1671-7414.2024.05.012]
　XIONG Xiaoqina,FENG Xiaoyua,MAO Aijuna,et al.Study on the Predictive Value of Serum Hsa-miR-30c-5p Level Expression in Patients with Type 2 Diabetes Mellitus for Microvascular Complications[J].Journal of Modern Laboratory Medicine,2024,39(05):63-68.[doi:10.3969/j.issn.1671-7414.2024.05.012]
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2 型糖尿病患者血清hsa-miR-30c-5p 水平表达对微血管并发症的预测价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年05期
页码:
63-68
栏目:
论著
出版日期:
2024-09-15

文章信息/Info

Title:
Study on the Predictive Value of Serum Hsa-miR-30c-5p Level Expression in Patients with Type 2 Diabetes Mellitus for Microvascular Complications
文章编号:
1671-7414（2024）050-063-06
作者:
熊小芹a; 冯潇宇a; 毛爱珺a; 马 丁a; 石亚军b
（巴中市中心医院 a. 内分泌科；b. 感染科，四川巴中 636000）
Author(s):
XIONG Xiaoqina;  FENG Xiaoyua;  MAO Aijuna;  MA Dinga;  SHI Yajunb
（a.Department of Endocrinology；b.Department of Infection, Bazhong Central Hospital, Sichuan Bazhong 636000, China）
关键词:
2 型糖尿病; 微血管并发症; 人微小核糖核酸-30c-5p
分类号:
R587.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.05.012
文献标志码:
A
摘要:
目的 探讨2 型糖尿病（type 2 diabetes mellitus，T2DM） 患者血清人微小核糖核酸（homo sapiensmicroRNA，hsa-miR）-30c-5p 表达对微血管并发症的预测价值。方法 选取2021 年5 月～ 2022 年9 月巴中市中心医院收治的T2DM 患者205 例为糖尿病组，并根据患者微血管并发症情况将糖尿病组进一步分为并发组（n ＝ 124）和未并发组（n ＝ 81），另选取同期健康体检者205 例为对照组，均采用逆转录- 聚合酶链反应（reverse transcriptionpolymerasechain reaction，RT-PCR）检测受试者血清hsa-miR-30c-5p 表达并进行比较。采用多因素Logistic 回归分析影响微血管并发症的因素，绘制受试者工作特征（receiver operating characteristic，ROC）曲线预测血清hsa-miR-30c-5p 表达对T2DM 患者发生微血管并发症的价值。结果 并发组、未并发组的血清hsa-miR-30c-5p 表达分别为0.58 ± 0.06，0.72± 0.08，均低于对照组（0.89 ± 0.21），差异具有统计学意义（t ＝ 16.038，7.079，均P ＝ 0.001）；并发组低于未并发组，差异具有统计学意义（t ＝ 14.289，P ＝ 0.001）。糖尿病病程[OR（95%CI）：3.873（2.976 ～ 4.770）]、尿酸[OR（95%CI）：2.125（1.211 ～ 3.040）]、糖化血红蛋白[OR（95%CI）：2.680（1.745 ～ 3.616）] 均为T2DM 患者发生微血管并发症的独立危险因素（均P ＜ 0.05），葡萄糖目标范围内时间[OR（95%CI）：0.491（0.135 ～ 0.846）]、血清hsa-miR-30c-5p[OR（95%CI）：0.532（0.146 ～ 0.817）] 均为T2DM患者发生微血管并发症的保护因素（均P ＜ 0.05）。血清hsa-miR-30c-5p 表达预测T2DM 患者发生微血管并发症的敏感度、特异度、曲线下面积（95% 置信区间）分别为81.45%，85.19%，0.802（0.741 ～ 0.854）。结论 T2DM 患者血清hsa-miR-30c-5p 表达异常降低，且血清hsa-miR-30c-5p 为T2DM 患者发生微血管并发症的保护因素，对T2DM 患者发生微血管并发症具有一定的预测价值。
Abstract:
Objective To explore the predictive value of serum homo sapiens microRNA (hsa-miR) -30c-5p expression in patients with type 2 diabetes mellitus (T2DM) for microvascular complications. Methods A total of 205 T2DM patients admitted to Bazhong Central Hospital from May 2021 to September 2022 were selected as the diabetes group, and the diabetes group was further divided into diabetes with combined group (n =124) and non combined group (n = 81) according to the microvascular complications of the patients. In addition, 205 healthy people who underwent physical examination during the same period were selected as the control group. The expression of hsa-miR-30c-5p in serum was detected by reverse transcription-polymerase chain reaction（RT-PCR）and compared. The factors affecting microvascular complications were analyzed using multivariate logistic regression analysis, and receiver operating characteristic (ROC) curves were plotted to predict the value of serum hsa-miR-30c-5p expression in predicting microvascular complications in T2DM patients. Results The expression of serum hsa-miR-30c-5p in the combined group (0.58 ± 0.06) and the non-combined group (0.72 ± 0.08) were lower than that in the control group (0.89 ± 0.21), and the differences were significant (t = 16.038, 7.079, all P = 0.001). The combined group was lower than the non-combined group, and the difference was significant (t = 14.289, P = 0.001). The course of diabetes [(OR (95%CI): 3.873 (2.976 ～ 4.770)], uric acid [(OR (95%CI): 2.125 (1.211 ～ 3.040)] and glycosylated hemoglobin [(OR (95%CI): 2.680 (1.745 ～ 3.616)] were independent risk factors for microvascular complications in T2DM patients (all P < 0.05), while the time within the target range of glucose [(OR (95%CI): 0.491 (0.135 ～ 0.846)] and serum hsamiR- 30c-5p [(OR (95%CI): 0.532 (2.976 ～ 4.770)] were protective factors for microvascular complications in T2DM patients (all P < 0.05). The sensitivity, specificity and area under the curve（95% CI） of serum hsa-mir-30c-5p expression in predicting microvascular complications in T2DM patients were 81.45%, 85.19% and 0.802(0.741 ～ 0.854), respectively. Conclusion The expression of serum hsa-miR-30c-5p in patients with T2DM is abnormally reduced, and serum hsa-miR-30c- 5p is a protective factor for microvascular complications in patients with T2DM. It may have a certain predictive value for microvascular complications in patients with T2DM.

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备注/Memo

备注/Memo:
作者简介： 熊小芹（1980-），女，本科，副主任医师，研究方向：糖尿病、骨质疏松、甲状腺疾病、甲状旁腺疾病、垂体、肾上腺、性腺疾病，E-mail：xiongxiaoqin1980x@163.com。
更新日期/Last Update: 2024-09-15