[1]刘春稳a,王 月b,刘欣悦c,等.宫颈癌组织中LNMAS mRNA,CLDN4 mRNA水平表达与上皮间质转化的相关性及临床意义研究[J].现代检验医学杂志,2024,39(06):90-95.[doi:10.3969/j.issn.1671-7414.2024.06.015]
 LIU Chunwena,WANG Yueb,LIU Xinyuec,et al.Correlation between the Expression of LNMAS mRNA and CLDN4 mRNA Levels in Cervical Cancer Tissue and Epithelial-Mesenchymal Transition and Clinical Significance[J].Journal of Modern Laboratory Medicine,2024,39(06):90-95.[doi:10.3969/j.issn.1671-7414.2024.06.015]
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宫颈癌组织中LNMAS mRNA,CLDN4 mRNA水平表达与上皮间质转化的相关性及临床意义研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年06期
页码:
90-95
栏目:
论著
出版日期:
2024-11-15

文章信息/Info

Title:
Correlation between the Expression of LNMAS mRNA and CLDN4 mRNA Levels in Cervical Cancer Tissue and Epithelial-Mesenchymal Transition and Clinical Significance
文章编号:
1671-7414(2024)06-090-06
作者:
刘春稳a王 月b刘欣悦c徐闻铂c孙志敏c
(沧州市妇幼保健院 a. 妇女体检与健康管理科;b. 产一科;c. 妇科,河北沧州 061000)
Author(s):
LIU Chunwena WANG Yueb LIU Xinyuec XU Wenboc SUN Zhiminc
(a. Department of Women’s Physical Examination and Health Management ; b.the First Department of Obstetrics; c. Department of Gynaecology, Cangzhou Maternal and Child Health Hospital, Hebei Cangzhou 061000, China)
关键词:
宫颈癌长链非编码RNA 淋巴结转移相关抑制因子紧密连接蛋白4上皮间质转化
分类号:
R737.33;R730.43
DOI:
10.3969/j.issn.1671-7414.2024.06.015
文献标志码:
A
摘要:
目的 研究宫颈癌组织长链非编码RNA(long non coding RNA,lncRNA)淋巴结转移相关抑制因子(lymphnode metastasis-associated suppressor,LNMAS)mRNA, 紧密连接蛋白4(claudin 4,CLDN4)mRNA 表达与上皮间质转化(epithelia-mesenchymal transition,EMT)的相关性及临床意义。方法 选取2018 年6 月~ 2020 年6 月在沧州市妇幼保健院首次诊治的96 例宫颈癌患者。实时荧光定量PCR(qRT-PCR)检测宫颈癌组织LNMAS mRNA,CLDN4 mRNA 及EMT 相关基因的表达。采用Pearson 相关分析LNMAS mRNA,CLDN4 mRNA 与VIM mRNA,E-cadmRNA,N-cad mRNA 的相关性。K-M 曲线分析LNMAS mRNA,CLDN4 mRNA 表达对宫颈癌患者三年生存率的影响。COX回归分析宫颈癌预后的影响因素。结果 相比于癌旁组织,宫颈癌组织中LNMAS mRNA(1.13±0.28 vs 1.97±0.37),E- 钙黏素(E-cad) mRNA(1.02±0.33 vs 2.29±0.56)表达显著降低(t= 17.738,19.144),CLDN4 mRNA(3.14±0.52vs 1.19±0.28),N- 钙黏素(N-cad) mRNA(2.60±0.36 vs 1.02±0.33)及波形蛋白(VIM)mRNA(2.84±0.46 vs 1.25±0.33)表达显著升高(t=32.351,26.951,27.518),差异具有统计学意义(均P < 0.05)。Pearson 相关分析显示,宫颈癌组织LNMAS mRNA与E-cad mRNA 呈正相关(r=0.712,P< 0.05),与VIM mRNA,N-cad mRNA呈负相关(r=-0.654,-0.589,均P < 0.05);CLDN4 mRNA 与E-cad mRNA 呈负相关(r= -0.668,P < 0.05),与VIM mRNA,N-cad mRNA 呈正相关(r=0.714,0.749,均P < 0.05)。相比于FIGO 分期Ⅰ A ~Ⅰ B1 期、无淋巴结转移患者宫颈癌组织LNMAS mRNA 表达较FIGO 分期Ⅰ B2 ~Ⅱ A 期、有淋巴结转移宫颈癌组织较低(t=12.288,10.228),CLDN4 mRNA 表达较高(t=13.636,11.771),差异具有统计学意义( 均P<0.05)。Log-Rankχ2 检验比较,LNMAS mRNA 高、低表达组三年总生存率分别为92.00%(46/50),60.87%(28/46);CLDN4 mRNA 高、低表达组三年总生存率分别为59.18%(29/49),95.74%(45/47),差异具有统计学意义(Log-Rank χ2=11.030,15.600,均P< 0.001)。FIGO分期ⅠB2~ⅡA期(HR=1.119,95%CI:1.148 ~ 1.830)、淋巴结转移(HR=1.442,95%CI:1.124 ~ 1.850)、CLDN4 mRNA(HR=1.637,95%CI:1.239 ~ 2.163)是影响宫颈癌患者预后的危险因素,LNMAS mRNA(HR=0.637,95%CI:0.465 ~ 0.873)是保护因素(均P<0.05)。结论 宫颈癌组织中LNMAS mRNA 表达降低,CLDN4 mRNA 表达升高,均与EMT 过程有关,是评估宫颈癌患者预后的标志物。
Abstract:
Objective To investigate the correlation between the expression of long non-coding RNA lymph node metastasisassociated suppressor (LNMAS) mRNA and tight junction protein claudin 4 (CLDN4) mRNA and epithelial-mesenchymal transition (EMT) in cervical cancer and the clinical significance. Methods A total of 96 patients with cervical cancer who were first diagnosed and treated at Cangzhou Maternal and Child Health Hospital from June 2018 to June 2020 were selected. Realtime fluorescent quantitative PCR was used to detect the expressions of LNMAS mRNA, CLDN4 mRNA and EMT-related genes. Pearson correlation analysis was used to analyze the correlation between LNMAS mRNA, CLDN4 mRNA and VIM mRNA, E-cad mRNA, N-cad mRNA. The impact of LNMAS mRNA and CLDN4 mRNA expression on the 3-year survival rate of cervical cancer patients was analyzed using the K-M curve. Cox regression was used to analyze prognostic factors for cervical cancer. Results Compared to adjacent tissues, the expressions of LNMAS mRNA (1.13±0.28 vs. 1.97±0.37) and E-cadherin (E-cad) mRNA(1.02±0.33 vs 2.29±0.56) in cervical cancer tissues were lower(t=17.738, 19.144), while CLDN4 mRNA (3.14±0.52 vs 1.19±0.28), N-cadherin (N-cad) mRNA(2.60±0.36 vs 1.02±0.33) and Vimentin (VIM) mRNA(2.84±0.46 vs 1.25±0.33) were higher(t=32.351, 26.951,27.518), with significant differences (all P < 0.05). LNMAS mRNA showed a positive correlation with E-cad mRNA (r=0.712, P < 0.05), but a negative correlation with VIM mRNA and N-cad mRNA in cervical cancer tissues (r=-0.654, -0.589, all P < 0.05). Additionally, the expression of CLDN4 mRNA was negatively correlated with E-cad mRNA (r=-0.668, P < 0.05), but positively correlated with VIM mRNA and N-cad mRNA (r=0.714, 0.749, all P < 0.05). Compared with patients with FIGO stage IA~IB1 and no lymph node metastasis, patients with FIGO stage IB2-IIA and lymph node metastasis exhibited a lower LNMAS mRNA expression and a higher CLDN4 mRNA expression with significant differences (t=12.288, 10.228, 13.636, 11.771). Comparison Log-Rank χ2 square test, the 3-year overall survival rates for high and low expression groups of LNMAS mRNA were 92.00% (46/50) and 60.87% (28/46), respectively; the 3-year overall survival rates for high and low expression groups of CLDN4 mRNA were 59.18% (29/49) and 95.74% (45/47), respectively, and the difference between the two groups were significant (log-Rank χ2=11.030, 15.600, all P < 0.001). FIGO stage IB2~IIA (HR=1.119, 95%CI:1.148~1.830), lymph node metastasis (HR=1.442, 95%CI:1.124~1.850) and CLDN4 mRNA (HR=1.637, 95%CI: 1.124~1.850) were risk factors affecting the prognosis of cervical cancer patients, while LNMAS mRNA (HR=0.637, 95%CI: 0.465~0.873) was a protective factor (all P<0.05). Conclusion The expression of LNMAS mRNA is decreased, while the expression of CLDN4 mRNA is increased in cervical cancer. The expressions of LNMAS mRNA and CLDN4 mRNA were associated with the process of EMT, which may be markers for evaluating the prognosis of patients with cervical cancer.

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备注/Memo

备注/Memo:
基金项目:沧州市重点研发计划指导项目(项目编号:222106053),河北省医学科学研究课题计划(项目编号:20241789)。
作者简介:刘春稳(1987-),女,本科,主治医师,研究方向:宫颈癌的筛查与诊断,E-mail:liuchuwen126523@163.com。
更新日期/Last Update: 2024-11-15