[1]任建华a,张 然b,崔秀英a,等.急性缺血性脑卒中患者血清LncRNA RMST，miR-582-5p表达水平与早期神经功能恶化及预后的相关性研究[J].现代检验医学杂志,2025,40(01):64-68.[doi:10.3969/j.issn.1671-7414.2025.01.012]
　REN Jianhuaa,ZHANG Ranb,CUI Xiuyinga,et al.Correlation between Serum LncRNA RMST, miR-582-5p Expression Levels and Early Neurological Deterioration and Prognosis in Patients with Acute Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2025,40(01):64-68.[doi:10.3969/j.issn.1671-7414.2025.01.012]
点击复制

急性缺血性脑卒中患者血清LncRNA RMST，miR-582-5p表达水平与早期神经功能恶化及预后的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
64-68
栏目:
论著
出版日期:
2025-01-15

文章信息/Info

Title:
Correlation between Serum LncRNA RMST, miR-582-5p Expression Levels and Early Neurological Deterioration and Prognosis in Patients with Acute Ischemic Stroke
文章编号:
1671-7414（2025）01-064-05
作者:
任建华a; 张 然b; 崔秀英a; 赵 新a
（承德市中心医院 a. 神经诊断科；b. 神经内科，河北承德 067000）
Author(s):
REN Jianhuaa; ZHANG Ranb; CUI Xiuyinga; ZHAO Xina
(a. Department of Neurodiagnosis；b. Department of Neurology，Chengde Central Hospital，Hebei Chengde 067000，China)
关键词:
长链非编码RNA 横纹肌肉瘤相关转录物-2; 微小RNA-582-5p; 急性缺血性脑卒中; 神经功能恶化
分类号:
R743.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.01.012
文献标志码:
A
摘要:
目的 探讨急性缺血性脑卒中（AIS）患者血清长链非编码RNA 横纹肌肉瘤相关转录物-2（LncRNA RMST）和miR-582-5p 的表达水平与早期神经功能恶化（END）及预后的相关性。方法 选取2021 年1 月～ 2022 年12 月期间承德市中心医院收治的129 例AIS 患者，并根据患者一周内是否出现END 将患者分为END 组（n=42）和非END 组（n=87），另选取同期在该院体检的健康者59 例作为健康组。采用qRT-PCR 法检测各组血清LncRNA RMST，miR-582-5p 水平。Pearson 法对END 患者血清LncRNA RMST，miR-582-5p 水平相关性进行分析；绘制ROC 曲线评估血清LncRNA RMST，miR-582-5p 水平以及二者联合对AIS 患者预后情况的效能分析。结果 健康组、非END 组、END 组血清LncRNA RMST 水平（1.01±0.28，2.10±0.41，3.99±0.52）逐渐升高，miR-582-5p 水平（1.02±0.23，0.86±0.16，0.73±0.15）逐渐降低，差异具有统计学意义（F=672.974，31.907，均P ＜ 0.05）。END 患者血清LncRNA RMST水平与miR-582-5p 水平存在明显的负相关（r=-0.451，P<0.001）。血清LncRNA RMST，miR-582-5p 水平联合诊断AIS 患者预后的AUC（95%CI）为0.961(0.912 ～ 0.987)，优于单独预测（Z=2.280，4.515，均P ＜ 0.05）。结论 血清LncRNA RMST，miR-582-5p 水平与END 关系密切，有望成为AIS 患者预后的预测因子。
Abstract:
Objective To investigate the expression changes of serum long non-coding RNA rhabdomyosarcoma associated transcript-2 (LncRNA RMST) and miR-582-5p in patients with acute ischemic stroke (AIS), and their correlation with early neurological deterioration (END) and prognosis. Methods A total of 129 patients with ischemic stroke admitted to Chengde Central Hospital from January 2021 to December 2022 were gathered, according to diagnostic criteria and patients were divided into END group (n=42) and non END group (n=87) besed on whether END occurred within one week. In addition, 59 healthy individuals who underwent physical examination in the hospital were regarded as the health group. The real-time fluorescence quantitative polymerase chain reaction method was applied to detect serum LncRNA RMST and miR-582-5p levels in each group. The Pearson method was used to analyze the correlation between serum LncRNA RMST and miR-582-5p levels in END patients. ROC curve was applied to evaluate further the levels of blood LncRNA RMST and miR-582-5p, and the efficacy analysis of their combination in predicting the prognosis of AIS patients. Results Serum LncRNA RMST(1.01±0.28，2.10±0.41， 3.99±0.52) levels gradually increased in the healthy, non-END and END groups, and miR-582-5p(1.02±0.23，0.86±0.16， 0.73±0.15) levels gradually decreased，and the differences were statistically significant (F=672.974，31.907，all P<0.05). There was a significant negative correlation between serum LncRNA RMST levels were significantly negatively correlated with miR-582-5p levels (r=-0.451，P<0.001). The AUC（95%CI） of the combined diagnosis of serum LncRNA RMST and miR- 582-5p levels in AIS patients was 0.961(0.912 ～ 0.987), which was better than the independent prediction (Z=2.280，4.515, all P ＜ 0.05). Conclusion The levels of serum LncRNA RMST and miR-582-5p were closely related to END, and they are expected to become predictive factors for the prognosis of AIS patients.

参考文献/References:

[1] MENDELSON S J, PRABHAKARAN S. Diagnosis and management of transient ischemic attack and acute ischemic stroke: a review[J]. JAMA, 2021, 325(11): 1088-1098.
[2] LIU Peipei, LIU Shoufeng, FENG Ning, et al. Association between neurological deterioration and outcomes in patients with stroke[J]. Annals of Translational Medicine, 2020, 8(1): 4.
[3] YIN Dongliang, XU Furong, LU Ming, et al. Long non-coding RNA RMST promotes oxygen-glucose deprivation-induced injury in brain microvascular endothelial cells by regulating miR-204-5p/VCAM1 axis [J]. Life Sciences, 2021, 284:119244.
[4] DING Hongsheng, GAO Shan, WANG Lei, et al. Overexpression of miR-582-5p inhibits the apoptosis of neuronal cells after cerebral ischemic stroke through regulating PAR-1/Rho/Rho axis[J]. Journal of Stroke and Cerebrovascular Diseases, 2019, 28(1): 149-155.
[5] 彭斌，刘鸣，崔丽英．与时俱进的新指南-《中国急性缺血性脑卒中诊治指南2018》解读[J]．中华神经科杂志，2018，51（9）：657-659． PENG Bin, LIU Ming, CUI Liying. New evidence, new guideline: interpretation of the Chinese guidelines for diagnosis and treatment of acute ischemic stroke 2018[J]. Chinese Journal of Neurology, 2018, 51(9): 657-659.
[6] 张磊，刘建民．改良Rankin 量表[J]．中华神经外科杂志，2012，28（5）：512． ZHANG Lei, LIU Jianmin. Modified Rankin scale [J]. Chinese Journal of Neurosurgery, 2012, 28(5): 512.
[7] YAMAL J M, GROTTA J C. National institutes of health stroke scale as an outcome measure for acute stroke trials[J]. Stroke, 2021, 52(1): 142-143.
[8] 吴亚武，王志辉，李剑，等．急性大动脉粥样硬化型脑梗死早期神经功能恶化与血脂水平的关系[J]．解放军预防医学杂志，2019，37（7）：122-123． WU Yawu, WANG Zhihui, LI Jian, et al. Relationship between early neurologic deterioration and lipid levels in acute atherosclerotic cerebral infarction[J]. Journal of Preventive Medicine of Chinese People’s Liberation Army, 2019, 37(7): 122-123.
[9] RAJAGOPAL T, SESHACHALAM A, AKSHAYA R L, et al. Association of HOTAIR (rs920778 and rs1899663) and NME1 (rs16949649 and rs2302254) gene polymorphisms with breast cancer risk in India [J]. Gene, 2020, 762: 145033.
[10] 郎海雷，曹雷涛，贵英斌，等．LncRNA NNT-AS1 通过调控miR-582-5p/NCKAP1 轴激活Hippo-YAP/TAZ 信号通路促进膀胱癌细胞增殖、迁移、侵袭和干细胞干性影响[J]．现代检验医学杂志，2023，38（4）：27-34, 39. LANG Hailei, CAO Leitao, GUI Yingbin, et al. LncRNA NNT-AS1 activates Hippo-YAP/TAZ signaling pathway by regulating miR-582-5p/NCKAP1 axis to promote bladder cancer cell proliferation，migration，invasion and stem cell stemness effects[J]. Journal of Modern Laboratory Medicine, 2023, 38(4): 27-34, 39.
[11] LI Jie, WANG Ning, NIE Huan, et al. Long non-coding RNA RMST worsens ischemic stroke via microRNA-221-3p/PIK3R1/TGF-β signaling pathway[J]. Molecular Neurobiology, 2022, 59(5): 2808-2821.
[12] 王玉松，李培，饶国敏，等．LncRNA UCA1 靶向 miR-582-5p 调控缺血低氧性脑神经细胞存活的实验研究[J]．脑与神经疾病杂志，2023，31（9）：581-586． WANG Yusong, LI Pei, RAO Guomin, et al. LncRNA UCA1 regulates the survival of hypoxic-ischemic brain neurons by targeting miR-582-5p[J]. Journal of Brain and Nervous Diseases, 2023, 31(9): 581-586.

备注/Memo

备注/Memo:
基金项目：承德市科学技术研究与发展计划项目（编号：202204A010）。
作者简介：任建华（1982-），女，本科，主治医师，研究方向：神经、神经电生理，E-mail：rjn2damr@163.com。
通讯作者：张然（1981-），女，副主任医师，E-mail：zhangrancd@163.com。
更新日期/Last Update: 2025-01-15