[1]王思琦,吴 泽,余 楠.基于CRISPR-Cas12a系统的病原体核酸床旁检测技术方法研究的最新进展[J].现代检验医学杂志,2025,40(01):213-220.[doi:10.3969/j.issn.1671-7414.2025.01.041]
　WANG Siqi,WU Ze,YU Nan.Research Advances in Pathogen Nucleic Acid Point-of-Care Testing Techniques Based on CRISPR-Cas12a System[J].Journal of Modern Laboratory Medicine,2025,40(01):213-220.[doi:10.3969/j.issn.1671-7414.2025.01.041]
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基于CRISPR-Cas12a系统的病原体核酸床旁检测技术方法研究的最新进展()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
213-220
栏目:
综述
出版日期:
2025-01-15

文章信息/Info

Title:
Research Advances in Pathogen Nucleic Acid Point-of-Care Testing Techniques Based on CRISPR-Cas12a System
文章编号:
1671-7414（2025）01-213-08
作者:
王思琦; 吴 泽; 余 楠
（南方医科大学检验与生物技术学院，广州 510515）
Author(s):
WANG Siqi; WU Ze; YU Nan
(School of Laboratory Medicine and Biotechnology，Southern Medical University， Guangzhou 510515, China)
关键词:
成簇的规则间隔的短回文重复序列相关蛋白系统; 病原体核酸检测; 床旁检测
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2025.01.041
文献标志码:
A
摘要:
病原体核酸检测是精准诊断、治疗和预防感染性疾病的重要环节，即时、简便的床旁检测（POCT）的研制对于实现病原体核酸高效检测意义重大。一种基因编辑工具被发现应用于核酸检测方法高效而实用，此即成簇的规则间隔的短回文重复序列及其相关蛋白（CRISPR-Cas）系统，其中，结构简单的CRISPR-Cas12a 因其强大的靶向结合、反式切割基因片段功能而得以广泛应用。基于CRISPR-Cas12a 系统的病原体核酸检测方法，利用等温或变温核酸扩增技术以及光学信号作为检测介质的POCT 技术，具有高灵敏度、高特异度和检测流程简化等特点。该文主要介绍基于CRISPR-Cas12a 系统的病原核酸检测POCT 技术方法的最新研究进展，为研制快速、准确的病原体核酸检测方法提供新思路。
Abstract:
Pathogen nucleic acid detection is critical to diagnosing, treating and preventing infectious diseases. To efficiently conduct pathogen nucleic acid detection, the development of point-of-care testing(POCT) with immediacy and simplicity is of great significance. A highly effective and practical gene editing tool discovered for nucleic acid detection is the clustered regularly interspaced short palindromic repeats-CRISPR associated proteins (CRISPR-Cas) systems. Among them, CRISPR-Cas12a is widely used due to its simple structure and powerful functions of target binding and collateral cleavage of gene fragments. Based on the CRISPR-Cas12a system, the pathogen nucleic acid detection method utilizes isothermal and temperature-variable nucleic acid amplification techniques and optical signals as the detection medium for POCT. It features high sensitivity, specificity and simplified detection procedures. This article mainly introduces the latest research progress of pathogen nucleic acid detection POCT techniques based on the CRISPR-Cas12a system, providing new ideas for developing rapid and accurate pathogen nucleic acid detection methods.

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备注/Memo

备注/Memo:
基金项目 ：中国博士后科学基金（2021M701628）。
作者简介 ：王思琦（2003-），女，在读本科生，专业：医学检验技术，E-mail：1715166206@qq.com。
通讯作者 ：余 楠（1972-），女，主任技师，硕士生导师，主要从事病原微生物检测技术研究，E-mail：yunanzhujiang@163.com。
更新日期/Last Update: 2025-01-15