[1]李寿敏,兰冰雪,潘 莉,等.下调ACTG1抑制PI3K/Akt信号通路相关蛋白表达促进胃癌细胞HGC-27凋亡机制研究[J].现代检验医学杂志,2025,40(02):1-5.[doi:10.3969/j.issn.1671-7414.2025.02.001]
　LI Shoumin,LAN Bingxue,PAN Li,et al.Mechanism of ACTG1 Downregulation Inhibits the Expression of Protein Associated to PI3K/Akt Signaling Pathway and Promotes Gastric Cancer Cells Line HGC-27 Apoptosis[J].Journal of Modern Laboratory Medicine,2025,40(02):1-5.[doi:10.3969/j.issn.1671-7414.2025.02.001]
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下调ACTG1抑制PI3K/Akt信号通路相关蛋白表达促进胃癌细胞HGC-27凋亡机制研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
1-5
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Mechanism of ACTG1 Downregulation Inhibits the Expression of Protein Associated to PI3K/Akt Signaling Pathway and Promotes Gastric Cancer Cells Line HGC-27 Apoptosis
文章编号:
1671-7414（2025）02-001-05
作者:
李寿敏1; 2; 3; 兰冰雪1; 2; 潘 莉1; 2; 4; 孙 铭1; 2; 崔淼淼1; 2; 5; 韦四喜1; 2; 黄 海1; 2
(1. 贵州医科大学附属医院临床检验中心，贵阳550004；2. 贵州医科大学医学检验学院，贵阳550004；3. 六盘水市人民医院检验科，贵州六盘水553001；4. 贵阳市南明区人民医院检验科，贵阳550004；5. 贵阳市第二人民医院检验科，贵阳550004)
Author(s):
LI Shoumin1; 2; 3; LAN Bingxue1; 2; PAN Li1; 2; 4; SUN Ming1; 2; CUI Miaomiao1; 2; 5; WEI Sixi1; 2; HUANG Hai1; 2
（1.Center for Clinical Laboratories，the Affiliated Hospital of Guizhou Medical University，Guiyang 550004，China；2. School of Clinical Laboratory Science，Guizhou Medical University，Guiyang 550004，China；3. Department of Clinical Laboratory，Liupanshui Municipal People’s Hospital，Guizhou Liupanhui 553001，China；4. Department of Clinical Laboratory，Guiyang Nanming District People’s Hospital，Guiyang 550004，China；5. Department of Clinical Laboratory, Guiyang Second People’s Hospital，Guiyang 550004，China）
关键词:
γ- 肌动蛋白; 人胃癌细胞( 未分化); 磷脂酰肌醇3- 激酶/ 蛋白激酶B; 凋亡
分类号:
R735.2；R730.43
DOI:
10.3969/j.issn.1671-7414.2025.02.001
文献标志码:
A
摘要:
目的 探讨下调γ- 肌动蛋白(ACTG1) 对人胃癌细胞（未分化）（HGC-27）凋亡的影响及机制。方法 利用CRISPR/Cas9 技术构建稳定敲低ACTG1 HGC-27 细胞系；通过DNA 测序及Western blot 验证ACTG1 敲低情况；流式细胞术检测细胞凋亡率；Western blot 检测凋亡相关蛋白Bcl2 相关X 蛋白（Bax）、B 细胞淋巴瘤2 家族蛋白（Bcl2）以及PI3K（磷脂酰肌醇3- 激酶）/AKT（蛋白激酶B）通路相关蛋白AKT，p-AKT 的表达水平。结果 构建稳定敲低ACTG1 的HGC-27 细胞系。与Control 组相比，ACTG1 蛋白水平在sgACTG1-1 组和sgACTG1-2 组显著下降。与Control 组细胞凋亡率相比，sgACTG1-1 组和sgACTG1-2 组细胞凋亡率(10.11%±0.46% , 14.67%±0.17% vs6.54%±0.67%) 明显升高，差异具有统计学意义(t=-7.58，-20.28，均P<0.01)；与Control 组相比，sgACTG1-1 组和sgACTG1-2 组Bax 蛋白水平(0.89±0.02，1.00±0.08 vs 0.71±0.03) 显著升高(t=-8.14，-5.87)，Bcl2 蛋白水平(0.49±0.06, 0.39±0.06 vs 0.65±0.07) 显著下降(t=3.09，5.35)，差异具有统计学意义( 均 P<0.05)。与Control 组相比，sgACTG1-1 组和sgACTG1-2 组AKT 蛋白水平(0.95±0.10 , 0.43±0.09 vs 1.17±0.06) 和p-AKT 蛋白水平(0.38±0.08,0.28±0.12 vs 0.70±0.14) 显著下降，差异具有统计学意义(t=3.20，12.13；3.44，3.85，均 P<0.05)。结论 下调ACTG1抑制PI3K/Akt 信号通路相关蛋白AKT，p-AKT 的表达，促进胃癌细胞HGC-27 凋亡。
Abstract:
Objective To investigate the effect and mechanism of actin gamma1 (ACTG1) downregulation on apoptosis in gastric cancer cells line HGC-27 . Methods Stable ACTG1 knockdown HGC-27 cell line was constructed by CRISPR/Cas9 , ACTG1 knockdown was verified by DNA sequencing and Western blot , apoptosis rate was detected by flow cytometry , Western blot detected the expression level of apoptosis-related proteins Bcl2-associated X protein(Bax) , B cell lymphoma 2 family protein (Bcl2) and PI3K/AKT signal pathway-related proteins AKT and p-AKT. Results HGC-27 cell lines with stable knockdown of ACTG1 were constructed, and the ACTG1 protein levels were decreased in the sgACTG1-1 and sgACTG1-2 groups compared with the Control group. Compared with the apoptosis rate in the Control group (6.54%±0.67%), cell apoptosis rate in the sgACTG1-1 and sgACTG1-2 groups (10.11%±0.46%, 14.67%±0.17%) were significantly increased , the differences were statistically significant (t=-7.58, -20.28, all P <0.01). Compared the Control group, the Bax protein levels in the sgACTG1-1 and sgACTG1-2 groups (0.89±0.02, 1.00±0.08 vs 0.71±0.03) were significantly increased (t= -8.14, -5.87), the level of Bcl2 protein (0.49±0.06, 0.39±0.06 vs 0.65±0.07) were significantly decreased(t=3.09, 5.35), the differences were statistically significant (all P<0.05). Compared with the Control group, the AKT protein levels in the sgACTG1-1 and sgACTG1-2 groups (0.95±0.10, 0.43±0.09 vs 1.17±0.06) and the P-AKT protein level (0.38±0.08, 0.28±0.12 vs 0.70±0.14) were significantly decreased, the differences were statistically significant (t=3.20, 12.13; 3.44, 3.85, all P <0.05). Conclusion Downregulation of ACTG1 inhibits the expression of PI3K / Akt signaling pathway related proteins AKT, p-AKT and promotes gastric cancer cells line HGC-27 apoptosis .

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备注/Memo

备注/Memo:
基金项目： 国家自然科学基金(82060442)；2021 年贵州医科大学附属医院博士科研启动基金(gyfybsky-2021-39)；贵州医科大学国家自然科学基金培育项目(21NSFCP05)； 2021 年贵州医科大学附属医院国家自然科学基金培育项目(gyfynsfc-2021-11)。
作者简介：李寿敏(1987-)，男，主治医师，硕士研究生( 在读)，主要从事消化道肿瘤标志物研究，E-mail：378219117@qq.com。
通讯作者：黄海(1975-)，男，教授，博士研究生导师，主要从事消化道肿瘤标志物研究，E-mail：huanghai828@gmc.edu.cn。
更新日期/Last Update: 2025-03-15