[1]吴康乐,王玮蓉,杨树龙,等.兰州地区献血人群人细小病毒B19感染状况调查和基因型分析[J].现代检验医学杂志,2025,40(02):59-63.[doi:10.3969/j.issn.1671-7414.2025.02.011]
　WU Kangle,WANG Weirong,YANG Shulong,et al.Prevalence and Phylogenetic Analysis of Human Parvovirus B19 among Blood Donors in Lanzhou[J].Journal of Modern Laboratory Medicine,2025,40(02):59-63.[doi:10.3969/j.issn.1671-7414.2025.02.011]
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兰州地区献血人群人细小病毒B19感染状况调查和基因型分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
59-63
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Prevalence and Phylogenetic Analysis of Human Parvovirus B19 among Blood Donors in Lanzhou
文章编号:
1671-7414（2025）02-059-05
作者:
吴康乐1，王玮蓉1，杨树龙1，冯惟萍1，潘 登1，郭 逸2，张支凤1
（1. 甘肃省红十字血液中心，兰州 730046；2. 陕西省血液中心，西安710061 ）
Author(s):
WU Kangle1，WANG Weirong1，YANG Shulong1，FENG Weiping1，PAN Deng1，GUO Yi2，ZHANG Zhifeng1
（1.Gansu Red Cross Blood Center，Lanzhou 730046，China； 2. Shaanxi Blood Center，Xi’an 710061，China）
关键词:
献血人群; 人细小病毒B19; 基因型; 兰州地区
分类号:
R373.1；Q786
DOI:
10.3969/j.issn.1671-7414.2025.02.011
文献标志码:
A
摘要:
目的 调查兰州地区献血人群中人细小病毒B19（B19）的感染状况并分析其基因型特征，为指导制定献血人群B19 筛查策略提供依据。方法 通过实时荧光定量PCR 法（qRT-PCR）对2023 年4 ～ 10 月兰州地区5 722 例献血者样本进行B19 DNA 筛查，同步酶联免疫法筛查B19 IgG 和B19 IgM 抗体383 份，对B19 DNA 阳性样本进行病毒载量测定和VP1 序列测序，运用N-J 法构建进化树。结果 兰州地区B19 DNA，IgG 抗体和IgM 抗体的阳性率分别为0.47%（27/5 722），25.59%（98/383）和0.26%（1/383），三者均呈阳性的样本占0.26%（1/383），仅B19 DNA 阳性占0.52%（2/383），IgG 抗体阳性占19.06%（73/383），二者均阳性占6.27%（24/383）；病毒载量为4.24 IU/ml ～ 5.67×102 IU/ml，且所有样本均低于104 IU/ml。B19 DNA 阳性率在性别方面差异无统计学意义（χ2=0.86，P=0.35），而在年龄组方面差异具有统计学意义（χ2=8.00，P=0.02）；B19 IgG 抗体阳性率在性别方面差异具有统计学意义（χ2=5.03，P=0.02），而在年龄组方面差异无统计学意义（χ2=0.51，P=0.77）；B19 IgM 抗体阳性率在性别方面差异无统计学意义（χ2=2.84，P=0.09）。以VP1 序列构建系统进化树并分析表明，兰州地区的B19 毒株形成了一个独立的支系，属于基因型1，且以基因型1b 为主。结论 兰州地区献血人群B19 DNA 和IgM 抗体阳性率低，病毒载量亦较低，输血感染B19 的风险较小。而B19 IgG 抗体阳性率较高，建议密切关注该地区B19 感染情况，定期监测献血人群B19 感染率，并追踪B19 DNA 阳性献血者至受血者的情况，保障临床输血安全。
Abstract:
Objective To examine the serological and molecular prevalence as well as genotype characteristics of human Parvovirus B19 blood donors in Lanzhou, and to provide evidence for developing a screening strategy to reduce the risk of blood transfusion transmission. Methods A total of 5 722 blood samples collected from Lanzhou blood donors from April 2023 to October 2023 were tested for B19 DNA using real-time quantitative PCR(qRT-PCR). Additionally, 383 samples were screened for anti-B19 IgG and anti-B19 IgM using synchronous enzyme-linked immunoassay(ELISA). Viral load and VP1 sequencing were conducted on the B19 DNA-positive samples and the Neighbor-Joining (N-J) method was used to construct an evolutionary tree for the sequenced samples. Results The prevalence of human Parvovirus B19 DNA, IgG antibody and IgM antibody was 0.47%（27/5 722）, 25.59%(98/383) and 0.26%(1/383), respectively, and the samples positive for B19 DNA, IgG antibody and IgM antibody were 0.26% (1/383). The co-positivity rate for B19 DNA and IgG antibody was 6.27% (24/383), while the positivity rates for B19 DNA or IgG antibody alone were 0.52% (2/383) and 19.06% (73/383), respectively. Viral loads ranged from 4.24 IU/ml to 5.67×102 IU/ml, all below 104 IU/ml. There was no statistical significance in the positive rate of B19 DNA in gender (χ2=0.86, P=0.35), but there was statistical significance in the positive rate of B19 DNA among all age groups (χ2=8.00, P =0.02). The highest positive rate of B19 DNA was 0.65% in the 18 ～ 30 age group. There was statistical significance in the positive rate of B19 IgG antibody in gender (χ2=5.03, P =0.02), but there was no statistical significance in the positive rate of B19 IgG antibody among all age groups (χ2=0.51, P =0.77). The highest positive rate of B19 IgG antibody was 29.09% in the age group of 41 to 60. There was no significant difference in the positive rate of B19 IgM antibody in gender (χ2=2.84, P =0.09). The highest positive rate of B19 IgM was 3.85% in the age group of 18 ～ 30 years old. Based on the VP1 sequence, the phylogenetic tree revealed that B19 strains in Lanzhou formed a distinct cladistic lineage within genotype 1, predominantly represented by genotype 1b. Conclusion The prevalence of B19 DNA and IgM antibodies among blood donors in the Lanzhou area is low, and so is the viral load. Therefore the risk of transmitting B19 through blood transfusion is relatively small. Since the prevalence of B19 IgG antibody is high, it is suggested to closely monitor the transmission situation in the area, regularly monitor the prevalence of B19 among blood donors, and track the situation of B19 DNA-positive blood donors to recipients to ensure the safety of clinical blood transfusion.

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备注/Memo

备注/Memo:
基金项目：甘肃省卫生健康行业科研计划项目(GSWSKY2020-26) 。
作者简介：吴康乐(1984-)，男，硕士，主管技师，研究方向：血液筛查及实验室管理， E-mail：my1242@126.com。
通讯作者：郭逸(1984-)，男，硕士，主管技师，研究方向：输血技术与血液病毒检测研究，E-mail：guoyi1221@163.com。
张支凤（1964-），男，主任技师，研究方向：血站实验室质量管理, E-mail: 1213113606@qq.com，共同通讯作者。
更新日期/Last Update: 2025-03-15