[1]刘美燕,李 娜,赵淑洁,等.人参皂苷Rg3通过RhoA/ROCK/NLRP3通路改善糖尿病肾病小鼠肾小球内皮损伤机制的实验研究[J].现代检验医学杂志,2025,40(02):123-128.[doi:10.3969/j.issn.1671-7414.2025.02.023]
 LIU Meiyan,LI Na,ZHAO Shujie,et al.Experimental Study on the Mechanism of Ginsenoside Rg3 Improving Glomerular Endothelial Injury in Diabetic Nephropathy Mice Through RhoA/ROCK/NLRP3 Pathway[J].Journal of Modern Laboratory Medicine,2025,40(02):123-128.[doi:10.3969/j.issn.1671-7414.2025.02.023]
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人参皂苷Rg3通过RhoA/ROCK/NLRP3通路改善糖尿病肾病小鼠肾小球内皮损伤机制的实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
123-128
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Experimental Study on the Mechanism of Ginsenoside Rg3 Improving Glomerular Endothelial Injury in Diabetic Nephropathy Mice Through RhoA/ROCK/NLRP3 Pathway
文章编号:
1671-7414(2025)02-123-06
作者:
刘美燕1李 娜2赵淑洁1郑倩倩1霍云涛3
(1. 献县中医医院肾病科,河北沧州 062250;2. 定州市人民医院肾内科,河北定州 073000;3. 邯郸市磁县人民医院中药科,河北邯郸 056500)
Author(s):
LIU Meiyan1LI Na2ZHAO Shujie1ZHENG Qianqian1HUO Yuntao3
(1. Department of Nephrology, Xianxian Hospital of Traditional Chinese Medicine Hospital,Hebei Cangzhou 062250,China;2. Department of Nephrology,Dingzhou People’s Hospital,Hebei Dingzhou 073000,China;3. Department of Traditional Chinese Medicine,Hebei Handan City Cixian People’s Hospital, Hebei Handan 056500,China)
关键词:
糖尿病肾病肾小球内皮损伤人参皂苷Rg3Ras 同源基因家族成员A/RHO 关联卷曲螺旋蛋白激酶/ 含NLR 家族Pyrin 域蛋白3细胞焦亡
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2025.02.023
文献标志码:
A
摘要:
目的 探究人参皂苷Rg3 是否可通过Ras 同源基因家族成员A(RhoA)/RHO 关联卷曲螺旋蛋白激酶(ROCK)/含NLR 家族Pyrin 域蛋白3(NLRP3) 通路改善糖尿病肾病(DN)小鼠肾小球内皮损伤。方法 将40 只小鼠随机分为4组:对照组、DN 组、人参皂苷(人参皂苷Rg3)组及RhoA/ROCK 通路抑制(FD)组,每组10 只。血糖仪检测小鼠空腹血糖(FPG);ELISA 检测尿蛋白、尿素氮(BUN)和血肌酐(SCr)水平;PAS 染色检测肾小球形态结构并评估肾小球损伤指数(GDI);免疫荧光染色检测肾小球血小板- 内皮细胞黏附分子(PECAM-1 或CD31)、血管性血友病因子(vWF)、RhoA,ROCK 及细胞焦亡相关蛋白NLRP3 蛋白表达;Western blotting 检测肾小球中细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)与细胞焦亡相关的炎性因子白细胞介素-1β(IL-1β)及IL-18 蛋白表达。结果 与对照组相比,DN 组小鼠FPG,尿蛋白、SCr 及BUN 水平增加,差异具有统计学意义(t=17.59 ~ 43.81,均P<0.05);肾小球结构明显损伤且GDI 增加(t=20.73,P<0.05),肾小球中CD31,RhoA,ROCK,NLRP3 表达增加,vWF 表达减少;肾组织中ICAM-1,VCAM-1,IL-1β 及IL-18 表达增加,差异具有统计学意义(t=27.95 ~ 40.10,均P<0.05)。与DN组相比,人参皂苷组小鼠FPG,尿蛋白、BUN及SCr 水平减少,差异具有统计学意义(t=14.87 ~ 20.33,均P<0.05);肾小球结构损伤改善且GDI 减少(t=19.80,P<0.05);肾小球CD31,RhoA,ROCK 及NLRP3 表达减少,vWF 表达增加,FD 组小鼠肾组织ICAM-1,VCAM-1,IL-1β 及IL-18 表达减少,差异具有统计学意义(t=12.62 ~ 39.68,均P<0.05)。结论 人参皂苷Rg3 可通过下调RhoA/ROCK/NLRP3 通路,改善DN 小鼠肾小球内皮损伤及细胞焦亡水平。
Abstract:
Objective To investigate whether ginsenoside Rg3 can ameliorate glomerular endothelial injury in diabetic nephropathy(DN) mice through Ras homologous gene family member A(Rho A)/Rho-associated coiled-coil forming protein kinase, (ROCK1)/NLR family pyrin domain protein 3(NLRP3) pathway. Methods Forty mice were randomly divided into 4 groups: control group, DN group, ginsenoside (ginsenoside Rg3) group and RhoA/ROCK pathway inhibition (FD) group, with 10 mice in each group. Fasting blood glucose(FBG)was measured by glucose meter. The levels of urinary protein, urea nitrogen (BUN) and serum creatinine (SCr) were detected by ELISA. PAS staining was used to detect glomerular morphology and structure and to evaluate glomerular injury index (GDI). The expression of platelet-endothelial cell adhesion molecule (PECAM-1 or CD31), von Willefibrilia factor (vWF), RhoA, ROCK and NLRP3 protein related to pyrodeath were detected by immunofluorescence staining. Western blotting detected the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), the inflammatory factor interleukin-1β (IL-1β) and IL-18 protein in the glomerulus. Results Compared with the control group, the levels of FPG, urinary protein, BUN and SCr in DN group were increased,and the differences were statistically significant (t=17.59 ~ 43.81,all P<0.05). The glomerular structure was significantly damaged and GDI was increased (t=20.73, P<0.05). The expressions of CD31, RhoA, ROCK and NLRP3 in glomeruli were increased, while the expression of vWF was decreased. The expressions of ICAM-1, VCAM-1, IL-1β and IL-18 in renal tissues were increased, and the differences were statistically significant (t=27.95 ~ 40.10,all P<0.05). Compared with the DN group, the levels of FPG, urinary protein, BUN and SCr in ginsenoside group were decreased, and the differences were statistically significant (t=14.87 ~ 20.33, all P<0.05). The damage of glomerular structure was improved and GDI was decreased (t=19.80, P<0.05), the expression of CD31, RhoA, ROCK and NLRP3 in glomerular was decreased, and the expression of vWF was increased. The expressions of ICAM-1 , VCAM-1 , IL-1β and IL-18 in renal tissues of FD group were decreased, and the differences were statistically significant (t=12.62 ~ 39.68,all P<0.05). Conclusion Ginsenosides Rg3 can improve the level of glomerular endothelial injury and pyroptosis in DN mice by down-regulating RhoA/ROCK/NLRP3 pathway.

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备注/Memo

备注/Memo:
基金项目:河北省中医药类科学研究课题计划项目(2024200)。
作者简介:刘美燕(1989-),女,研究生,中西医结合主治医师,研究方向:肾病,E-mail:linlinpang13260@163.com。
通迅作者:赵淑洁(1985-),女,本科,副主任医师,研究方向:肾病,E-mail:wenmi5601950@163.com。
更新日期/Last Update: 2025-03-15