[1]刘伶俐,魏若宣,陈 蔚,等.miR-146b调控ERK1/2-AP-1信号通路参与糖尿病并发脑梗死大鼠模型的分子机制研究[J].现代检验医学杂志,2025,40(02):135-139.[doi:10.3969/j.issn.1671-7414.2025.02.025]
　LIU Lingli,WEI Ruoxuan,CHEN Wei,et al.Molecular Mechanism of miR-146b Regulating ERK1/2-AP-1 Signaling Pathway Involved in the Rat Model of Diabetes Complicated with Cerebral Infarction[J].Journal of Modern Laboratory Medicine,2025,40(02):135-139.[doi:10.3969/j.issn.1671-7414.2025.02.025]
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miR-146b调控ERK1/2-AP-1信号通路参与糖尿病并发脑梗死大鼠模型的分子机制研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年02期
页码:
135-139
栏目:
论著
出版日期:
2025-03-15

文章信息/Info

Title:
Molecular Mechanism of miR-146b Regulating ERK1/2-AP-1 Signaling Pathway Involved in the Rat Model of Diabetes Complicated with Cerebral Infarction
文章编号:
1671-7414（2025）02-135-05
作者:
刘伶俐1; 魏若宣2; 陈 蔚1; 孔彩霞1; 刘志红1
（1．武汉市第一医院/ 武汉市中西医结合医院内分泌科，武汉 430022；2．新疆医科大学中医学院，乌鲁木齐830000）
Author(s):
LIU Lingli1; WEI Ruoxuan2; CHEN Wei1; KONG Caixia1; LIU Zhihong1
（1．Department of Endocrinology， Wuhan No1. Hospital / Wuhan Hospital of Traditional Chinese and Western Medicine, Wuhan 430022，China； 2. College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830000，China)
关键词:
糖尿病并发脑梗死; 微小RNA-146b; 调控细胞外调节蛋白激酶; 活化蛋白-1
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2025.02.025
文献标志码:
A
摘要:
目的 探究miR-146b 是否可通过调控细胞外调节蛋白激酶（ERK1/2） 活化蛋白-1（AP-1）信号通路参与糖尿病并发脑梗死（DM-CI）大鼠的脑损伤过程。方法 将80 只SD 大鼠随机分为假手术组、DM-CI 组、低表达miR-146b组和抑制ERK1/2 组，每组20 只。美国国立卫生研究院卒中量表（NIHSS）评分衡量大鼠脑功能。RT-qPCR 检测大鼠脑组织中miR-146b，ERK1/2，AP-1 的mRNA 水平；Western blotting 检侧大鼠脑组织中ERK1/2，AP-1 的蛋白水平；TTC 染色检测大鼠脑梗死体积；H&E 染色检测大鼠脑组织病理学变化。血糖仪检测大鼠随机血糖水平。结果 与假手术组相比，DM-CI 组大鼠脑组织中miR-146b，ERK1/2，AP-1 的mRNA 表达水平显著增加，差异具有统计学意义（t=10.86，15.62，9.87，均P<0.05）；ERK1/2，AP-1 的蛋白水平增加，差异具有统计学意义（t=11.18，23.81，均P<0.05）；NIHSS 评分、随机血糖水平增加（t=44.49，30.02，均P<0.05）；脑梗死体积增加（t=51.05，P<0.05），脑组织结构紊乱且疏松，部分细胞周围间隙可见水肿。与DM-CI 组相比，低表达miR-146b 组大鼠脑组织中miR-146b，ERK1/2，AP-1mRNA 表达水平减少，差异具有统计学意义（t=38.00，20.03，24.25，均P<0.05）；ERK1/2，AP-1 蛋白表达减少，差异具有统计学意义（t=12.30，26.70，均P ＜ 0.05）；NIHSS 评分减少、随机血糖水平减少，差异具有统计学意义（t=38.11，33.77，均P<0.05），脑梗死体积减少（t=16.70，P<0.05），脑组织损伤及水肿程度改善。抑制ERK1/2 组大鼠脑组织中ERK1/2，AP-1 的蛋白及mRNA 表达水平减少，差异具有统计学意义（t=13.61 ～ 38.00，均P<0.05），大鼠NIHSS评分、随机血糖水平减少，差异具有统计学意义（t=16.48，26.61，P<0.05）。结论 miR-146b 可通过调控ERK1/2 AP-1通路参与DM-CI 大鼠的脑功能结构损伤过程。
Abstract:
Objective To explore whether miR-146b can participate in the brain injury process of diabetic rats with cerebral infarction (DM-CI) by regulating the extracellular regulatory protein kinase (ERK1/2) -activated protein-1 (AP-1) signaling pathway. Methods 80 SD rats were randomly divided into sham operation group, DM-CI group, low miR-146b expression group and ERK1/2 inhibition group, with 20 rats in each group. The National Institutes of Health Stroke Scale (NIHSS) score measures brain function in rats. The mRNA levels of miR-146b, ERK1/2 and AP-1 in rat brain tissue were detected by RT-qPCR. Western blotting detected ERK1/2, AP-1 protein levels in rat brain tissue. TTC staining was used to detect cerebral infarction volume in rats. H&E staining was used to detect brain histopathological changes. Random blood glucose levels were detected by glucose meter in rats. Results Compared with sham operation group, mRNA expression levels of miR-146b, ERK1/2 and AP-1 in brain tissue of rats in DM-CI group were significantly increased，with statistically differences (t=10.86, 15.62, 9.87, all P<0.05)． ERK1/2 and AP-1 protein levels increased，with statistically differences (t=11.18, 23.81, P<0.05)．NIHSS score increased and random blood glucose level increased (t=44.49, 30.02, all P<0.05), and increased cerebral infarction volume (t=51.05, P<0.05), the structure of brain tissue was disorganized and loose, and edema can be seen in the pericellular space．Compared with the DM-CI group, the mRNA expression levels of miR-146b, ERK1/2 and AP-1 in the brain tissue of rats with low expression of miR-146b were decreased，with statistically differences (t=38.00, 20.03, 24.25, all P<0.05)．the protein expression of EPK1/2 and AP-1 decreased, and the differences were statistically significant (t=12.30, 26.70, all P ＜ 0.05). NIHSS score and random blood glucose level were decreased，with statistically differences (t=38.11, 33.77, all P<0.05), cerebral infarction volume decreased (t=16.70, P<0.05), the degree of brain tissue in jury and edema was improved, and the expression levels of ERK1/2 and AP-1 protein and mRNA in brain tissue of rats inhibited by ERK1/2 were decreased，with statistically differences (t=13.61 ～ 38.00, all P<0.05), the NIHSS score of rats was decreased, and the random blood glucose level was decreased，with statistically differences (t=16.48, 26.61, all P<0.05). Conclusion MiR-146b may be involved in brain functional and structural damage in DM-CI rats by regulating ERK1/2-AP-1 signaling pathway.

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备注/Memo

备注/Memo:
基金项目：湖北省卫健委课题研究项目（WJ2023M122），武汉市卫健委科研项目（WX18B01）。
作者简介：刘伶俐（1983-），女，主治医师，研究方向：内分泌代谢，E-mail：menguanliao424056@163.com。
更新日期/Last Update: 2025-03-15