[1]马慧敏,周晓静,卢东娥,等.轮状病毒胃肠炎患儿血清ACTR2，DDX3X表达水平及其对病情严重程度评估价值研究[J].现代检验医学杂志,2025,40(03):108-112.[doi:10.3969/j.issn.1671-7414.2025.03.020]
　MA Huimin,ZHOU Xiaojing,LU Donge,et al.Expression Levels of Serum ACTR2 and DDX3X in Children with Rotavirus Gastroenteritis and Their Value in Evaluating the Severity of the Disease[J].Journal of Modern Laboratory Medicine,2025,40(03):108-112.[doi:10.3969/j.issn.1671-7414.2025.03.020]
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轮状病毒胃肠炎患儿血清ACTR2，DDX3X表达水平及其对病情严重程度评估价值研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年03期
页码:
108-112
栏目:
论著
出版日期:
2025-05-15

文章信息/Info

Title:
Expression Levels of Serum ACTR2 and DDX3X in Children with Rotavirus Gastroenteritis and Their Value in Evaluating the Severity of the Disease
文章编号:
1671-7414（2025）03-108-05
作者:
马慧敏，周晓静，卢东娥，闫玉姣
（邯郸市妇幼保健院小儿神经内科，河北邯郸056000 ）
Author(s):
MA Huimin，ZHOU Xiaojing，LU Donge，YAN Yujiao
（Department of Pediatric Neurology，Handan Maternal and Child Health Hospital，Hebei Handan 056000，China）
关键词:
轮状病毒胃肠炎; 肌动蛋白相关蛋白2; DEAD-box RNA解旋酶3X连锁; 病情严重程度
分类号:
R373.25；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.03.020
文献标志码:
A
摘要:
目的分析肌动蛋白相关蛋白2（ACTR2）、DEAD-boxRNA解旋酶3X连锁（DDX3X）表达对轮状病毒胃肠炎（RVGE）患儿病情严重程度的评估价值。方法选取2022年9月～2024年9月在邯郸市妇幼保健院收治的153例RVGE患儿为研究对象，根据RVGE病情严重程度将RVGE患儿分为轻型组（n=60）、中型组（n=71）和重型组（n=22）。另随机选取同期健康儿童60例为对照组。比较轻型组、中型组和重型组的临床资料；实时荧光定量PCR（RT-qPCR）法检测各组血清中ACTR2，DDX3X的表达水平；受试者工作特征（ROC）曲线分析血清ACTR2，DDX3X联合检测对重症RVGE患儿的评估价值。结果重型组RVGE患儿年龄明显低于轻型组、中型组患儿，差异具有统计学意义（Z=8.307，5.885，均P＜0.001）；三组患儿脱水情况、腹泻次数比较，差异具有统计学意义（F=9.434，126.080，均P＜0.05）。RVGE组ACTR2（1.20±0.28），DDX3X（1.22±0.37）表达水平高于对照组（1.01±0.02，1.04±0.09），差异具有统计学意义（t=5.071，3.584，均P<0.05）；轻型组、中型组和重型组血清中ACTR2[1.11±0.23，1.23±0.21，1.42（1.25，1.57）]，DDX3X[1.14±0.22，1.25±0.24，1.32（1.23，1.62）]的表达水平依次升高，差异具有统计学意义（Z=27.196，18.013，均P＜0.001）。ROC曲线分析结果表明，血清ACTR2，DDX3X联合预测重症RVGE患儿的AUC（95%CI），敏感度、特异度均高于两者单独检测，差异具有统计学意义（Z=2.573，2.101，P=0.014，0.034）。结论血清ACTR2，DDX3X表达水平升高均与RVGE病情严重程度密切相关，两者联合检测对于诊断RVGE病情严重程度具有较高的预测价值。
Abstract:
Objective To analyze the value of actin-related protein 2 (ACTR2) and DEAD-box RNA helicase 3X-linked (DDX3X) expression in evaluating the severity of Rotavirus gastroenteritis (RVGE) in children. Methods A total of 153 children with rotavirus gastroenteritis admitted to Handan Maternal and Child Health Hospital from September 2022 to September 2024 were selected as the research objects. According to the severity of RVGE, the children with RVGE were divided into mild group （n=60）, moderate group （n=71） and severe group（n=22）.In addition, 60 healthy children were randomly selected as the healthy group. The clinical data of the light group, the medium group and the heavy group were compared. The expression levels of ACTR2 and DDX3X in serum of each group were detected by real-time fluorescence quantitative PCR. ROC curve was used to analyze the evaluation value of combined detection of serum ACTR2 and DDX3X in children with severe RVGE. Results The age of children with RVGE in the severe group was significantly lower than that in the mild group and the moderate group, and the differences were statistically significant (Z=8.307，5.885，all P＜0.001). There were statistically significant differences in dehydration and diarrhea among the three groups (F=9.434, 126.080，all P＜0.05). The expression levels of ACTR2 (1.20±0.28） and DDX3X （1.22±0.37) in RVGE group were significantly higher than those in control group (1.01±0.02, 1.04±0.09), and the differences were statistically significant (t=5.071, 3.584, all P ＜ 0.05).The expression levels of ACTR2[1.11±0.23, 1.23±0.21, 1.42(1.25, 1.57)] and DDX3X[1.14±0.22, 1.25±0.24, 1.32(1.23, 1.62)] in the serum of the mild group , the moderate group and the severe group increased in turn, and the differences were statistically significant (Z=27.196, 18.013, all P＜0.05). The ROC curve analysis results showed that the combined detection of serum ACTR2 and DDX3X predicted the AUC(95% CI) of critically ill RVGE patients, with higher sensitivity and specificity than the detection of the two alone (Z=2.573, 2.101, P=0.014, 0.034). Conclusion The expression levels of serum ACTR2 and DDX3X are closely related to the severity of RVGE. The combined detection of the two has a high predictive value for the diagnosis of the severity of RVGE.

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备注/Memo

备注/Memo:
基金项目：河北省卫生健康委科研项目，项目编号：20230631。
作者简介：马慧敏（1984-），女，大学本科，主治医师，研究方向：神经系统、呼吸系统及消化系统疾病，E-mail：mhm741738796@qq.com。
通讯作者：周晓静（1983-），女，大学本科，副主任医师，研究方向：小儿神经系统疾病，E-mail：WQheng62o@163.com。
更新日期/Last Update: 2025-05-15