[1]倪 勇,陈丹丹,龚美娜,等.两种化学发光免疫分析系统对血清HBsAg突变株的检测能力比较分析[J].现代检验医学杂志,2025,40(03):195-198,220.[doi:10.3969/j.issn.1671-7414.2025.03.036]
　NI Yong,CHEN Dandan,GONG Meina,et al.Comparative Analysis of Two Chemiluminescent Immunoassay Systems for the Detection of Serum HBsAg Mutants[J].Journal of Modern Laboratory Medicine,2025,40(03):195-198,220.[doi:10.3969/j.issn.1671-7414.2025.03.036]
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两种化学发光免疫分析系统对血清HBsAg突变株的检测能力比较分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年03期
页码:
195-198,220
栏目:
研究简报·实验技术
出版日期:
2025-05-15

文章信息/Info

Title:
Comparative Analysis of Two Chemiluminescent Immunoassay Systems for the Detection of Serum HBsAg Mutants
文章编号:
1671-7414（2025）03-195-05
作者:
倪 勇1，陈丹丹1，龚美娜1，倪 想2
（1．芜湖市中医医院检验科，安徽芜湖 241000；2．海南医学院医学检验专业，海南海口570100）
Author(s):
NI Yong1，CHEN Dandan1，GONG Meina1，NI Xiang2
（1.Department of Clinical Laboratory，Wuhu Traditional Chinese Medicine Hospital，Anhui Wuhu 241000，China；2.Department of Medical Laboratory，Hainan Medical College，Haikou 570100，China）
关键词:
乙肝表面抗原; 突变体; 化学发光免疫分析; 荧光定量PCR
分类号:
R512.62；Q503
DOI:
10.3969/j.issn.1671-7414.2025.03.036
文献标志码:
A
摘要:
目的分析比较两种化学发光免疫分析系统对HBsAg突变株的检测能力。方法分别用I3000和LiaisonXL两种化学发光免疫分析系统对9种已知HBsAg突变位点的HBsAg突变株进行检测，比较这两种检测系统对9种HBsAg突变株的检测能力；对I3000系统检测HBsAg显示为阴性的临床血清样本，用LiaisonXL系统进行HBsAg复核，针对LiaisonXL系统阳性检测结果的HBsAg突变株设计qRT-PCR扩增引物，并对前述两种系统检测阴阳结果不一致临床样本进行荧光定量PCR和乙肝五项检测。结果LiaisonXL检测9种已知HBsAg突变位点的HBsAg突变株结果全部为阳性，而I3000可检测其中6种为阳性，有3种阴性结果。利用LiaisonXL系统检测1520例I3000检测系统HBsAg阴性的住院患者样本，发现4例阳性结果，其中有1例荧光定量PCR实验阳性，属于突变株P124L-F/Y143H-D144E-G145R的HBsAg突变体，且患者的HBeAb和HBcAb的检测数值较高，提示该患者为小三阳。结论LiaisonXL可检测出临床样本中P124L-F/Y143H-D144E-G145RHBsAg突变株，选择HBsAg突变株识别捕获能力强的HBsAg检测试剂有非常重要意义。
Abstract:
Objective To analyze and compare the ability of two chemiluminescent immunoassay systems for detecting HBsAg mutant strains. Methods The two chemiluminescent immunoassay systems, I3000 and Liaison XL, were used to detect nine HBsAg mutant strains with known HBsAg mutation sites respectively, and to compare the detection ability of these two systems for the nine HBsAg mutant strains. The clinical serum samples that were negative for HBsAg by the I3000 system were reviewed by the Liaison XL system for HBsAg, qRT-PCR amplification primers were designed for the HBsAg mutant strains with positive results by the Liaison XL system, and fluorescence quantitative PCR and Hepatitis B serologic tests were performed on the clinical samples that had inconsistent negative-positive results between the two aforementioned systems. Results The Liaison XL detected all nine HBsAg mutant strains with known HBsAg mutation sites with positive results, whereas the I3000 detected six of them with positive but three with negative results. Using the Liaison XL system to test 1 520 samples from hospitalized patients who were negative for HBsAg with the I3000 detection system, four positive results were found, including one positive result by the fluorescence quantitative PCR assay, which belonged to the HBsAg mutant of the mutant strain P124L-F/Y143H-D144E-G145R and the patient’s test values for HBeAb and HBcAb were high, suggesting that the patient was a minor triple positive. Conclusion Liaison XL can detect the P124L-F/Y143H-D144E-G145R HBsAg mutant in clinical samples, and it is very important to select HBsAg test reagents with strong HBsAg mutant recognition and capture ability.

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备注/Memo

备注/Memo:
基金项目：安徽省自然科学基金项目（2008085QH407）；芜湖市科技项目（2022yf70）。
作者简介：倪勇（1971-），男，大学本科，副主任技师，主要从事临床检验工作，E-mail: 13955353323@163.com。
更新日期/Last Update: 2025-05-15