[1]李 静,孙继芳,刘 苗,等.弱精子症患者精子鞭毛微管蛋白糖基化水平检测及其临床价值研究[J].现代检验医学杂志,2025,40(05):145-148,166.[doi:10.3969/j.issn.1671-7414.2025.05.027]
　LI Jing,SUN Jifang,LIU Miao,et al.Detection of the Level of Sperm Flagellar Tubulin Glycosylation in Asthenospermia and Evaluate the Clinical Value[J].Journal of Modern Laboratory Medicine,2025,40(05):145-148,166.[doi:10.3969/j.issn.1671-7414.2025.05.027]
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弱精子症患者精子鞭毛微管蛋白糖基化水平检测及其临床价值研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年05期
页码:
145-148,166
栏目:
论著
出版日期:
2025-09-15

文章信息/Info

Title:
Detection of the Level of Sperm Flagellar Tubulin Glycosylation in Asthenospermia and Evaluate the Clinical Value
文章编号:
1671-7414（2025）05-145-05
作者:
李 静a，孙继芳b，刘 苗a，艾尔代尼a，王志勇c，刘瑞军c，武 燕a，童卓云b
新疆维吾尔自治区妇幼保健院a.生殖医学中心；b.遗传室；c.男科，乌鲁木齐 830000
Author(s):
LI Jinga, SUN Jifangb, LIU Miaoa, AI Erdainia, WANG Zhiyongc, LIU Ruijunc, WU Yana, TONG Zhuoyunb
a. Department of Reproductive Medicine Center; b. Department of Genetique Factorielle; c. Department of Andrology, Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital, Urumqi 830000, China
关键词:
不育症; 弱精子症; 微管蛋白糖基化; 精子鞭毛
分类号:
R698.2；R446.19
DOI:
10.3969/j.issn.1671-7414.2025.05.027
文献标志码:
A
摘要:
目的探讨弱精子症患者（AP）精液中精子鞭毛微管蛋白糖基化（Gly-Tubulin）水平表达情况及临床意义。方法根据纳入排除标准，选择2021年6月～2022年5月在新疆维吾尔自治区妇幼保健院男科就诊的108例不育患者作为研究对象。依据精液常规分析结果分为AP组（n=74）和正常对照（NC）组（n=34）。收集两组患者年龄、不育年限等临床资料；采用化学发光法检测两组外周血血清性激素五项水平；免疫印迹法（Westernblot）检测两组精子鞭毛Gly-Tubulin蛋白水平；免疫荧光法对鞭毛Gly-Tubulin定位检测；多因素Logistic回归分析精子运动活力减弱的可能危险因素，分析精子Gly-Tubulin与AP的关系。结果?与NC组相比，AP组不育年限延长（3.98±0.30vs0.78±0.08），精子前向运动百分比降低（18.55%±0.97%vs46.24%±1.89%）、鞭毛Gly-Tubulin表达水平降低（0.64±0.11vs1.00±0.00），差异具有统计学意义（t=62.038，χ2=14.394，3.148，均P＜0.05）。两组卵泡刺激素（FSH）、促黄体生成素（LH）、泌乳素（PRL）、雌二醇（E2）、睾酮、精液体积、精子浓度、精子总数差异无统计学意义（t/χ2=0.043～1.503，均P＞0.05）。多因素Logistic回归分析表明精子前向运动、鞭毛Gly-Tubulin表达水平均是弱精子症的独立危险因素（Waldχ2=4.406，7.492，均P<0.05）。结论精子鞭毛Gly-Tubulin水平的异常可能与弱精子症的发生和发展密切相关。
Abstract:
Objective To examine the level of sperm flagellar tubulin glycosylation(Gly-Tubulin) in asthenospermia patients(AP) and discuss its significance. Methods According to the principle of inclusion and exclusion criteria, 108 patients treated in the Department of Andrology in Xinjiang Uygur Autonomous Region Maternal and Child Health Hospital from June 2021 to May 2022 were rolled in this study. According to the results of semen examine, they were divided into group AP (n=74), and normal control (NC) group (n=34). Colleced the clinical date of both group, such as the age, the sterility period. The levels of sex hormones were detected by chemiluminescence method. The glycosylated Tubulin levels of the sperm were detected by Western blot and immunofluorescence was used for localization analysis, and the relationship between the Gly-Tubulin of sperm flagellar and asthenospermia was analyzed by Multivariate Logistic regression analysis. Results Compared to NC group, the sterility period of group AP was prolong (3.98±0.30 vs 0.78±0.08), the percent of forward sperm motility was decreased(18.55±0.97 vs 46.24±1.89) and the expression level of Gly-Tubulin in the sperm flagellar was decreased(0.64±0.11 vs 1.00±0.00), the differences with statistical significance (t=62.038, χ2=14.394, 3.148, all P＜0.05). There were no significant differences in follicle-stimulating hormone(FSH), luteinizing hormone(LH), prolactin(PRL), estradiol(E2), testosterone, semen volume, sperm concentration and total sperm between the two groups (t/χ2=0.043～1.503, all P>0.05). Multivariate Logistic regression analysis showed that the percent of forward sperm motility and the sperm flagellar Gly-Tubulin expression level were independent risk factors for male asthenospermia (Wald χ2=4.406, 7.492, P<0.05). Conclusion Abnormal level of Gly Tubulin in sperm flagella may be closely related to the occurrence and development of asthenospermia.

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备注/Memo

备注/Memo:
基金项目： 新疆维吾尔自治区卫生健康青年医学科技人才专项科研项目（WJWY-202213）；新疆维吾尔自治区妇幼保健院院级项目（202306001）。
作者简介：李静（1984-），女，硕士，高级实验师，研究方向：生殖医学、医学遗传学，E-mail：mylove033@126.com。
通讯作者：武燕（1981-），女，本科，副主任医师，研究方向：生殖医学、妇产科学，E-mall：471054868@qq.com。童卓云（1993-），女，硕士，检验技师，研究方向：弱精子症机制研究，E-m
更新日期/Last Update: 2025-09-15