[1]吴晓康,熊朝亮,尹佳锋,等.西安地区2 649例疑似百日咳患者Bp DNA检测及临床和实验室结果分析[J].现代检验医学杂志,2025,40(05):158-161,171.[doi:10.3969/j.issn.1671-7414.2025.05.030]
　WU Xiaokang,XIONG Chaoliang,YIN Jiafeng,et al.Analysis of Bp-DNA Detection and Clinical and Laboratory Results of 2 649 Suspected Pertussis Patients in Xi’an Area[J].Journal of Modern Laboratory Medicine,2025,40(05):158-161,171.[doi:10.3969/j.issn.1671-7414.2025.05.030]
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西安地区2 649例疑似百日咳患者Bp DNA检测及临床和实验室结果分析()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年05期
页码:
158-161,171
栏目:
论著
出版日期:
2025-09-15

文章信息/Info

Title:
Analysis of Bp-DNA Detection and Clinical and Laboratory Results of 2 649 Suspected Pertussis Patients in Xi’an Area
文章编号:
1671-7414（2025）05-158-05
作者:
吴晓康a，熊朝亮a，尹佳锋a，张 妮a，雷 珂a，靳 莉a，张 月a，睢雯婷a，陈 栋a，杨拴盈b，何 谦a
西安交通大学第二附属医院 a.检验科；b.呼吸与危重症医学科，西安 710004
Author(s):
WU Xiaokanga，XIONG Chaolianga，YIN Jiafenga，ZHANG Nia，LEI Kea，JIN Lia，ZHANG Yuea，SUI Wentinga， CHEN Donga，YANG Shuanyingb，HE Qiana
a. Department of Clinical Laboratory；b. Department of Respiratory and Critical Care Medicine，the Second Affiliated Hospital of Xi’an Jiaotong University，Xi’an 710004，China
关键词:
百日咳; DNA检测; 流行特征
分类号:
R378.42；R446.11
DOI:
10.3969/j.issn.1671-7414.2025.05.030
文献标志码:
A
摘要:
目的?分析西安地区2649例疑似百日咳感染者状况及实验室诊断指标的变化。方法收集2023年6月～2025年5月西安交通大学第二附属医院就诊疑似百日咳感染2649例患者作为研究对象。采集患者鼻咽拭子，应用聚合酶链反应（PCR）-荧光探针法进行百日咳核酸检测，并对实验室诊断指标进行分析。结果2649例样本中百日咳核酸阳性250例，阳性率9.44%；男性患者检出率9.37%（127/1356），女性患者检出率9.51%（123/1293），二者比较差异无统计学意义（χ2＝0.019，P=0.894）。不同年龄组阳性检出率的差异具有统计学意义（χ2＝46.473，P＜0.05），以7～19岁年龄组的阳性检出率最高（14.98%）。百日咳流行呈现以4～9月份为高峰的季节性特征。21.20%（53/250）的阳性患者为混合感染。1～14岁百日咳感染组白细胞计数（WBC）、淋巴细胞百分比（LYMP%）、淋巴细胞计数（LYMP#）高于肺炎支原体（MP）感染组（t=10.179，5.819，8.614）和呼吸道合胞病毒（RSV）感染组（t=16.570，2.618，7.185），差异具有统计学意义（均P＜0.05）。14岁以上百日咳感染组LYMP%，LYMP#高于MP感染组（t=3.275，2.319）和RSV感染组（t=2.401，4.617），差异具有统计学意义（均P＜0.05）。结论西安地区2023～2025年百日咳感染状况呈现出显著年龄、季节及实验室检查结果的特征，应进一步完善本地区百日咳监测体系，优化临床诊疗流程和加强百日咳疫苗接种的工作。
Abstract:
Objective To analyze the status of 2 649 suspected pertussis patients in Xi’an and the changes in laboratory diagnostic indi-cators. Methods 2 649 patients with suspected pertussis who visited the Second Affiliated Hospital of Xi’an Jiaotong University from June 2023 to May 2025 were collected as the research subjects. Nasopharyngeal swabs were collected from the patients, and pertussis nucleic acid was detected by polymerase chain reaction (PCR)-fluorescence probe method. Laboratory diagnostic indicators were an-alyzed. Results Among the 2 649 samples tested, 250 were positive for pertussis nucleic acid, with a positive detection rate of 9.44%. The detection rate in male patients was 9.37%（127/1 356）, and in female patients was 9.51% (123/1 293), with difference no was statis-tically significant between the two groups (χ2= 0.019, P = 0.894). There was a statistically significant difference in the positive detection rate among different age groups (χ2= 46.473, P<0.05), with the highest positive detection rate in the 7～19 age group (14.98%). The prevalence of pertussis showed a seasonal characteristic with a peak from April to September. 21.2% (53/250) of the positive patients were mixed infections. In the 1～14 age group, the white blood cell count (WBC), lymphocyte percentage (LYMP%), and lymphocyte count (LYMP#) in the pertussis infection group were higher than those in the Mycoplasma pneumoniae（MP）infection group (t=10.179， 5.819，8.614) and the Respiratory syncytial virus（RSV）infection group (t=16.570, 2.618, 7.185), and the differences were statistically significant (all P<0.01)，respectively. In the ＞14 age group, the LYMP% and LYMP# in the pertussis infection group were higher than those in the MP infection group (t= 3.275，2.319) and the RSV infection group (t=2.401，4.617), and the differences were statistically significant (all P<0.05)，respectively. Conclusion The pertussis infection status in Xi’an area from 2023 to 2025 shows significant char-acteristics in terms of age, season and laboratory test results. It is necessary to further improve the pertussis surveillance system in this area, optimize the clinical diagnosis and treatment process and strengthen the vaccination work of pertussis vaccine.

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相似文献/References:

[1]薛 达,王增国,蒋恺憧,等.百日咳重现下MRBp 感染的治疗、防控及面临的挑战[J].现代检验医学杂志,2024,39(04):1.[doi:10.3969/j.issn.1671-7414.2024.04.001]
　XUE Da,WANG Zengguo,JIANG Kaichong,et al.Treatment, Prevention and Challenges of MRBp Infection in the Resurgence of Pertussis[J].Journal of Modern Laboratory Medicine,2024,39(05):1.[doi:10.3969/j.issn.1671-7414.2024.04.001]

备注/Memo

备注/Memo:
基金项目：陕西省重点研发计划(2021SF-133)。
作者简介：吴晓康（1977-），男，研究生，副主任技师，主要从事感染性疾病分子诊断，E-mail：wxk111506@sohu.com。
更新日期/Last Update: 2025-09-15