[1]孙 曼,童胜兰,蒋在慧,等.脓毒症患者血清MEGF6，SAMSN1表达水平与急性肺损伤的相关性研究[J].现代检验医学杂志,2025,40(06):126-130.[doi:10.3969/j.issn.1671-7414.2025.06.023]
　SUN Man,TONG Shenglan,JIANG Zaihui,et al.Correlation between Serum MEGF6, SAMSN1 Expression Levels and Acute Lung Injury in Patients with Sepsis[J].Journal of Modern Laboratory Medicine,2025,40(06):126-130.[doi:10.3969/j.issn.1671-7414.2025.06.023]
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脓毒症患者血清MEGF6，SAMSN1表达水平与急性肺损伤的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年06期
页码:
126-130
栏目:
论著
出版日期:
2025-11-15

文章信息/Info

Title:
Correlation between Serum MEGF6, SAMSN1 Expression Levels and Acute Lung Injury in Patients with Sepsis
文章编号:
1671-7414（2025）06-126-05
作者:
孙 曼a，童胜兰b，蒋在慧b，丁道银c，向长港a
华中科技大学协和江南医院/武汉市江夏区第一人民医院a.检验科；b.感染性疾病科；c.重症医学科，武汉 430200
Author(s):
SUN Mana, TONG Shenglanb, JIANG Zaihuib, DING Daoyinc, XIANG Changganga
a.Department of Laboratory;b. Department of Infectious Diseases; c. Department of Intensive Care Medicine, Huazhong University of Science and Technology Union Jiangnan Hospital /Wuhan Jiangxia District First People’s Hospital), Wuhan 430200, China
关键词:
脓毒症; 急性肺损伤; 多表皮生长因子样结构域6; SAM域SH3域和核定位信号分子1
分类号:
R631；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.06.023
文献标志码:
A
摘要:
目的探讨脓毒症（sepsis）患者血清多表皮生长因子样结构域6（MEGF6），SAM域SH3域和核定位信号分子1（SAMSN1）表达水平与急性肺损伤（ALI）的关系。方法选择2021年2月～2024年2月就诊于华中科技大学协和江南医院的182例脓毒症患者（脓毒症组），根据是否并发ALI分为ALI组（n=60）和非ALI组（n=122）。以同期体检的80例健康人为对照组。采用酶联免疫吸附实验（ELISA）检测血清MEGF6，SAMSN1水平。Pearson相关性分析血清MEGF6，SAMSN1与临床指标的相关性；多因素Logistic回归分析脓毒症并发ALI的危险因素；受试者工作特征（ROC）曲线分析血清MEGF6，SAMSN1对脓毒症并发ALI的评估价值。结果脓毒症组血清MEGF6（37.52±7.87ng/L），SAMSN1（24.26±7.45ng/L）低于对照组（78.17±15.26ng/L，62.31±14.12ng/L），差异具有统计学意义（t=28.397，28.477，均P＜0.001）。ALI组血清MEGF6（29.22±7.64ng/L），SAMSN1（13.28±7.12ng/L）低于非ALI组（41.60±7.90ng/L，29.66±7.67ng/L），差异具有统计学意义（t=10.045，13.961，均P<0.05）。脓毒症并发ALI患者血清MEGF6，SAMSN1与APACHEⅡ评分、SOFA评分呈负相关（r=-0.811～-0.728，均P<0.05），与氧合指数（OI）呈正相关（r=0.689，0.640，均P<0.05）。APACHEⅡ评分、SOFA评分是脓毒症并发ALI的危险因素，血清MEGF6（OR=0.769，95%CI：0.612～0.965），SAMSN1（OR=0.778，95%CI：0.617～0.980）是保护因素（Waldχ2=5.140，4.525，均P＜0.05）。血清MEGF6，SAMSN1单独及联合检测对脓毒症并发ALI评估的曲线下面积（AUC）分别为0.840，0.811，0.903，联合检测大于单项检测，差异具有统计学意义（Z=4.602，4.318，均P＜0.05）。结论脓毒症患者血清MEGF6，SAMSN1水平降低，是脓毒症并发ALI的影响因素，两者联合检测对脓毒症并发ALI有较高的评估价值。
Abstract:
Objective To investigate the relationship between the expression levels of serum multiple epithelial growth factor like domains 6 (MEGF6), sterile alpha motif (SAM) domain, scr homology 3(SH3) domain and nuclear localization signals 1 (SAMSN1) and acute lung injury (ALI) in patients with sepsis. Methods A total of 182 patients with sepsis (sepsis group) who were admitted to Huazhong University of Science and Technology Union Jiangnan Hospital from February 2021 to February 2024 were selected and divided into ALI group (n=60) and non-ALI group (n=122) according to whether they were complicated with ALI or not. 80 healthy people who underwent physical examination during the same period were selected as the control group.Serum MEGF6 and SAMSN1 levels were detected by enzyme-linked immunosorbent assay(ELISA). Pearson correlation analysis was used to analyze the correlation between serum MEGF6, SAMSN1 and clinical indicators. Multivariate Logistic re-gression analysis was used to analyze the risk factors of sepsis complicated with ALI. The receiver operating characteristic(ROC) curve was used to analyze the value of serum MEGF6 and SAMSN1 in the evaluation of sepsis complicated with ALI. Results Serum MEGF6 (37.52±7.87 ng/L) and SAMSN1 (24.26±7.45 ng/L) in the sepsis group were lower than those in the control group(78.17±15.26 ng/L, 62.31±14.12 ng/L), and the differences were statistically significant (t=28.397, 28.477, all P<0.001). Serum MEGF6 (29.22±7.64 ng/L) and SAMSN1 (13.28±7.12 ng/L) in the ALI group were lower than those in the non-ALI group(41.60±7.90 ng/L, 29.66±7.67 ng/L), and the differences were statistically significant (t=10.045, 13.961, all P<0.05). Se-rum MEGF6 and SAMSN1 in patients with sepsis complicated with ALI were negatively correlated with APACHE II score and SOFA score (r=-0.811~ -0.728, all P<0.05), and positively correlated with oxygenation index(OI) (r=0.689, 0.640, all P<0.05). APACHE II score and SOFA score were risk factors for sepsis complicated with ALI. Serum MEGF6 (OR=0.769, 95%CI: 0.612~0.965) and SAMSN1 (OR=0.778, 95%CI: 0.617~0.980) were protective factors (Wald χ2=5.140, 4.525, all P<0.05). The area under the curve (AUC) of serum MEGF6, SAMSN1 alone and combined in the evaluation of sepsis complicated with ALI were 0.840, 0.811 and 0.903, respectively. The combined detection was greater than the single detection, and the differences were statistically significant (Z=4.602, 4.318, all P<0.05). Conclusion The serum levels of MEGF6 and SAMSN1 in patients with sepsis are decreased, which are the influ-encing factors of sepsis complicated with ALI. The combined detection of the two has a high evaluation value for sepsis complicated with ALI.

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备注/Memo

备注/Memo:
基金项目：湖北省卫生健康委员会科研项目（项目编号：WJ2021M042）。
作者简介：孙曼（1986-），女，本科，主管技师，研究方向：分子诊断、质量管理、临床基础检验方面，E-mail：sunm861018@163.com
。
通讯作者：向长港（1976-），男，本科，主任技师，研究方向：分子诊断、临床化学、质量管理方面，E-mail：xiangcg1897@163.com
。
更新日期/Last Update: 2025-11-15