[1]吴富炜,华文明,齐天琪,等.6种药敏试验方法检测耐碳青霉烯肺炎克雷伯菌对替加环素敏感度的评价研究[J].现代检验医学杂志,2025,40(06):159-164.[doi:10.3969/j.issn.1671-7414.2025.06.029]
　WU Fuwei,HUA Wenming,QI Tianqi,et al.Evaluation of 6 Drug Susceptibility Testing Methods in Detecting the Sensitivity of Carbapenem-resistant Klebsiella Pneumoniae to Tigecycline[J].Journal of Modern Laboratory Medicine,2025,40(06):159-164.[doi:10.3969/j.issn.1671-7414.2025.06.029]
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6种药敏试验方法检测耐碳青霉烯肺炎克雷伯菌对替加环素敏感度的评价研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年06期
页码:
159-164
栏目:
论著
出版日期:
2025-11-15

文章信息/Info

Title:
Evaluation of 6 Drug Susceptibility Testing Methods in Detecting the Sensitivity of Carbapenem-resistant Klebsiella Pneumoniae to Tigecycline
文章编号:
1671-7414（2025）06-159-06
作者:
吴富炜，华文明，齐天琪，杨靖娴
航天中心医院检验科，北京 100049
Author(s):
WU Fuwei，HUA Wenming，QI Tianqi，YANG Jingxian
Department of Clinical Laboratory, Aerospace Center Hospital, Beijing 100049, China
关键词:
耐碳青霉烯肺炎克雷伯菌; 替加环素; 药物敏感性试验
分类号:
R378.996；R446.5
DOI:
10.3969/j.issn.1671-7414.2025.06.029
文献标志码:
A
摘要:
目的评价6种商品化药敏试验方法检测耐碳青霉烯肺炎克雷伯菌（CRKP）对替加环素敏感度的准确性，为实验室选择合适药敏方法提供依据。方法收集航天中心医院2023年6月～2024年2月临床分离的非重复CRKP菌株，用Vitek-2仪器法筛选出替加环素中介[最低抑菌浓度（MIC）=4μg/ml]和耐药（MIC≥8μg/ml）的CRKP菌株各60例，分为中介组和耐药组，采用不同药敏试验方法[包括MIC测试条（MTS）法、Vitek-2仪器法、商品化肉汤稀释法、E试验法、纸片扩散法和纸片联合复敏液法]检测CRKP对替加环素的敏感度。以MTS法为参考方法，分别在美国食品与药品监督管理局（FDA）和欧洲药敏试验委员会（EUCAST）判读标准下，对其它5种药敏方法进行方法学评价。结果对中介组菌株，在FDA标准下，商品化肉汤稀释法和纸片联合复敏液法与MTS法一致性高，为可接受；而在EUCAST标准下，5种方法均不可接受；对耐药组菌株，在两种标准下，5种方法的CA均<90%，为不可接受。对全部菌株进行总体分析，结果与耐药组一致。结论?5种方法在当前样本量下均未达到可接受标准，尤其不适合MIC值较高菌株的复核。商品化肉汤稀释法与MTS法一致性最高，值得扩大样本量进一步研究评估。
Abstract:
Objective To evaluate the accuracy of 6 commercial antibiotic susceptibility testing methods in detecting the sensitivity of carbapenems-resistant Klebsiella pneumoniae (CRKP) to tigecycline, and to provide an evidence for clinical laboratory to select the appropriate tigecyline susceptibility tests. Methods Non-duplicate CRKP clinically isolated from Aerospace Center Hospital from June 2023 to February 2024 were collected. 60 cases of tigecycline intermediated CRKP [minimal inhibitery concentration (MIC)=4μg/ml] and 60 cases of drug-resistant CRKP (MIC≥8μg/ml) were screened by Vitek-2. The sensitivity of CRKP to tigecycline was detected by different methods. With the MIC test strip (MTS) as the reference method, the methodological evaluation of Vitek-2, commercial broth dilution method, E-test, Kirby-Bauer disk diffusion (KB) and disk combined with resensitization bufferwas carried out under the interpretation standards of the U S Food and Drug Administration (FDA) and the European Commission for Drug Susceptibility Testing (EUCAST), respectively. Results For the intermediate strains, under the FDA criteria, both the commercial broth dilution method and the disk combinedwith resensitization buffer method showed high consistency with the MTS method and were acceptable, whereas under the EUCAST criteria, all five methods were unacceptable. For the resistant strains, no matter what standard was, CA of all five methods were less than 90%, which were unacceptable. For all strains as a whole, the results were consistent with those of the resistant ones. Conclusion Under the current sample size, none of the five methods met the acceptable standards, especially not suitable for the recheck of strains with higher MICs. Commercial broth dilution method have the highest consistency with MTS, and it is worth expanding the sample size for further study.

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备注/Memo

备注/Memo:
基金项目：首都卫生发展科研专项（编号：首发2020-4-6084）。
作者简介：吴富炜（1985-），女，学士，副主任技师，研究方向：临床微生物检验，E-mail：564245665@qq.com。
通讯作者：杨靖娴（1985-），女，硕士，主任技师，研究方向：临床微生物检验，E-mail：yangjx5680@163.com。
更新日期/Last Update: 2025-11-15