[1]邓伟伟,唐成琼,马秀敏.沉默TIM-3基因调控TAMs极化对宫颈癌细胞增殖和迁移能力影响的实验研究[J].现代检验医学杂志,2026,41(01):21-23+34.[doi:10.3969/j.issn.1671-7414.2026.01.005]
　DENG Weiwei,TANG Chengqiong,MA Xiumin.Experimental Study of the Effect of TIM-3 Silencing-Regulated TAMs Polar-ization on the Proliferation and Migration of Cervical Cancer Cells[J].Journal of Modern Laboratory Medicine,2026,41(01):21-23+34.[doi:10.3969/j.issn.1671-7414.2026.01.005]
点击复制

沉默TIM-3基因调控TAMs极化对宫颈癌细胞增殖和迁移能力影响的实验研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年01期
页码:
21-23+34
栏目:
论著
出版日期:
2026-01-15

文章信息/Info

Title:
Experimental Study of the Effect of TIM-3 Silencing-Regulated TAMs Polar-ization on the Proliferation and Migration of Cervical Cancer Cells
文章编号:
1671-7414（2026）01-021-04
作者:
邓伟伟a，唐成琼b，马秀敏a
a 新疆医科大学附属肿瘤医院a.检验科；b.放射物理技术科，乌鲁木齐 830000
Author(s):
DENG Weiweia, TANG Chengqiongb, MA Xiumina
a. Department of Clinical Laboratory; b. Department of Radiation Physics and Technology, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830000, China
关键词:
T细胞免疫球蛋白黏蛋白‐3; 宫颈癌细胞; 侵袭迁移; 肿瘤相关巨噬细胞
分类号:
R737.33；R730.43
DOI:
10.3969/j.issn.1671-7414.2026.01.005
文献标志码:
A
摘要:
目的探讨沉默T细胞免疫球蛋白黏蛋白‐3（Tim-3）基因对肿瘤相关巨噬细胞（TAMs）极化的调控作用，及这种调控对宫颈癌细胞增殖与迁移能力的影响。方法?构建沉默TIM-3基因的TAMs模型，采用CCK-8实验检测宫颈癌细胞增殖能力，Transwell实验检测宫颈癌细胞迁移能力，蛋白质免疫印迹（Westernblotting）实验检测上皮间质转化（EMT）相关蛋白（上皮钙黏素，神经钙黏素）的表达变化，流式细胞术检测TAMs极化状态。结果沉默TIM-3的宫颈癌细胞增殖率、迁移率显著降低，差异具有统计学意义（t=4.17～12.81，均P<0.05）；E-cadherin蛋白表达量显著增高，N-cadherin蛋白表达量显著降低，差异具有统计学意义（t=8.90～25.00，均P<0.05）；CD86阳性细胞比例显著升高，CD163阳性细胞比例显著降低，差异具有统计学意义（t=11.92～26.10，均P<0.05）。结论沉默TIM-3基因能够有效调控TAMs极化，显著抑制宫颈癌细胞的增殖与迁移能力，为宫颈癌的靶向治疗提供了新的理论依据和潜在靶点。
Abstract:
Objective This study aim to investigate the regulatory impact of the T cell immunoglobulin and mucin domain-con-taining protein-3(TIM-3) gene silencing on the polarization of tumor-associated macrophages (TAMs) and its influence on the proliferation and migration of cervical cancer cells. Methods The experiment constructed a TAMs model with silenced TIM-3 gene, and used CCK-8 assay to detect the proliferation ability of cervical cancer cells,Transwell assay to detect the migration abil-ity of cervical cancer cells,Western blotting to detect the expression changes of epithelial-to-mesenchymal transition (EMT)-related proteins (E-cadherin, N-cadherin),and flow cytometry to detect the polarization state of TAMs. Results The proliferation and migration rates of cervical cancer cells with TIM-3 silencing were significantly decreased, and the differences were statistically significant (t= 4.17～12.81, all P<0.05). The protein expression level of E-cadherin was significantly increased , while that of N-cadherin was significantly decreased, and the differences were statistically significant (t=8.90～25.00, all P<0.05). The propor-tion of CD86-positive cells increased significantly, while the proportion of CD163-positive cells decreased significantly, and the differences were statistically significant (t=11.92～26.10, all P<0.05). Conclusions The TIM-3 gene silencing regulates effec-tively the polarization of tumor-associated macrophages (TAMs), thereby suppressing the proliferation and migration of cervical cancer cells. This finding provides a new theoretical foundation and a promising therapeutic target for the immunotherapy of cer-vical cancer.

参考文献/References:

[1] SUNG H, FERLAY J, SIEGEL R L, et al. Global cancer sta-tistics 2020: cervical cancer incidence and mortality[J]. CA: a Cancer Journal for Clinicians, 2021, 71(3): 209-249.
[2] GE Y J, ZHANG Y C, ZHAO K N, et al. Emerging therapeutic strategies of different immunotherapy approaches combined with PD-1/PD-L1 blockade in cervical cancer [J]. Drug Design, Development and Therapy, 2022, 16: 3055-3070.
[3] 汪祖春,王烈宏,祁青玲. AMPK/CCL18轴调控TAMs的M2型转化促进宫颈癌细胞增殖?迁移和侵袭的实验研究[J].现代检验医学杂志,2025,40(3):47-51, 58. WANG Z C, WANG L H, QI Q L. AMPK/CCL18 axis regulates the M2 transformation of TAMs, promoting proliferation, migration and invasion of cervical cancer cells[J]. Journal of Modern Laboratory Medicine, 2025, 40(3): 47-51, 58.
[4] ZHANG Z Y, REN C Y, XIAO R, et al. Palmitoylation of TIM-3 promotes immune exhaustion and restrains antitumor immunity[J]. Science Immunology, 2024, 9(101): eadp7302.
[5] DIXON K O, LAHORE G F, KUCHROO V K. Beyond T cell exhaustion: TIM-3 regulation of myeloid cells[J]. Science Immunology, 2024, 9(93): eadf2223.
[6] 孙文泽,李守帅. LncRNA LINC01137通过诱导CD8+T细胞耗竭促进非小细胞肺癌进展的机制研究[J].现代检验医学杂志,2024,39(2):1-6, 134. SUN W Z, LI S S. Mechanism study of lncRNA LINC01137 promoting the progression of non-small cell lung cancer by inducing CD8+T cell exhaustion[J]. Journal of Modern Laboratory Medicine, 2024, 39(2): 1-6, 134.
[7] ANDERSON A C, KUCHROO V K. TIM-3: a key regulator of immune exhaustion in cancer[J]. Nature Reviews Immunology, 2021, 21(3): 155-167.
[8] 赵彤,郭海平,郑勤.TIM-3表达对肝癌细胞的作用及其机制研究[J].肝脏,2024,29(6):671-675, 718. ZHAO T, GUO H P, ZHENG Q. The expression and mechanism of Tim-3 in hepatocellular carcinoma[J]. Chinese Hepatology, 2024, 29(6): 671-675, 718.
[9] TIAN W, LIU M, LIU Y Q, et al. TIM-3 regulates pro-liferation via BDNF-mediated PI3K/AKT axis in endo-metriosis[J]. Molecular Medicine, 2023, 129(1): 170.
[10] PAGLIANO O, MORRISON R M, CHAUVIN J M, et al. Tim-3 mediates T cell trogocytosis to limit antitumor immunity[J]. Journal of Clinical Investigation, 2022, 132(9): e152864.
[11] SCHATTON T, ITOH Y, MARTINS C, et al. Inhibition of melanoma cell-intrinsic Tim-3 stimulates MAPK-de-pendent tumorigenesis[J]. Cancer Research, 2022, 82(20): 3774-3784.
[12] SHEN Y, CHEN J, LI X P. Research advances in im-mune checkpoint drugs for non-small cell lung can-cer[J]. Journal of Drug Targeting, 2023, 31(7): 700-713.
[13] WANG Y P, ZHAO S J, ZHANG X L, et al. Higher T cell immunoglobulin mucin-3 (Tim-3) expression in cervical cancer is associated with a satisfactory progno-sis[J]. Translational Cancer Research, 2020, 9(4): 2801-2813.
[14] CHEN Z F, DONG D, ZHU Y J, et al. The role of TIM-3/Galectin-9 pathway in T-cell function and prognosis of patients with human papilloma virus-associated cervical carcinoma[J]. FASEB Journal, 2021, 35(3):e21401.
[15] SISTO M, RIBATTI D, LISI S. Cadherin signaling in cancer and autoimmune diseases[J]. International Jour-nal of Molecular Sciences, 2021, 22(24): 13358.
[16] VULETI? A, MIRJA?I? MARTINOVI? K, TI?MA MILETI? N, et al. Cross-talk between tumor cells un-dergoing epithelial to mesenchymal transition and natu-ral killer cells in tumor microenvironment in colorectal cancer[J]. Frontiers in Cell and Developmental Biology, 2021, 9: 750022.
[17] CAZZATO G, CASCARDI E, COLAGRANDE A, et al. Tcell immunoglobulin and mucin domain 3(TIM-3) in cutaneous melanoma:a narrative review[J]. Cancers, 2023, 15(6): 1697.
[18] VALENTE S, NASCIMENTO C, GAMEIRO A, et al. TIM-3 is a potential immune checkpoint target in Cats with mammary carcinoma[J]. Cancers, 2023, 15(2): 384.
[19] LU C L, LIU Y, MIAO L X, et al. Research progress on the role of tumor-associated macrophages in tumor de-velopment and their use as molecular targets (Review) [J]. International Journal of Oncology, 2024, 64(2): 11.
[20] MANTOVANI A, ALLAVENA P, MARCHESI F, et al. Macrophages as tools and targets in cancer therapy[J]. Na-ture Reviews Drug Discovery, 2022, 21(11): 799-820.

备注/Memo

备注/Memo:
基金项目：新疆维吾尔自治区自然科学基金资助项目（2022D01C507）。
作者简介：邓伟伟（1983-），女，硕士，副主任医师，主要从事肿瘤免疫的机制研究，E-mail：707905188@qq.com。
通讯作者：马秀敏（1968-），女，主任技师，博士生导师，主要从事病原微生物机制研究，E-mail：397211657@qq.com。
更新日期/Last Update: 2026-01-15