[1]李淑湘,郑维玲,石 燕,等.采用间接法建立苏州地区表观健康成人血清高尔基体蛋白73水平参考区间及验证[J].现代检验医学杂志,2026,41(01):161-164.[doi:10.3969/j.issn.1671-7414.2026.01.031]
　LI Shuxiang,ZHENG Weiling,SHI Yan,et al.Establishment and Validation of Reference Intervals of Golgi Protein 73 in Healthy Adults in Suzhou Area by Indirect Method[J].Journal of Modern Laboratory Medicine,2026,41(01):161-164.[doi:10.3969/j.issn.1671-7414.2026.01.031]
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采用间接法建立苏州地区表观健康成人血清高尔基体蛋白73水平参考区间及验证()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年01期
页码:
161-164
栏目:
论著
出版日期:
2026-01-15

文章信息/Info

Title:
Establishment and Validation of Reference Intervals of Golgi Protein 73 in Healthy Adults in Suzhou Area by Indirect Method
文章编号:
1671-7414（2026）01-161-04
作者:
李淑湘a，b，郑维玲a，b，石 燕a，b，魏衍财a，b
南京医科大学附属苏州医院/苏州市立医院 a.检验医学中心；b.苏州市智慧+重大疾病生物标志物研究与转化重点实验室，江苏苏州 215104
Author(s):
LI Shuxianga，b, ZHENG Weilinga，b, SHI Yana，b, WEI Yancaia，b
a. Laboratory Medicine Center; b. Suzhou Key Laboratory of Intelligent Critical Illness Biomarkers Translational Research, the Affiliated Suzhou Hospital of Nanjing Medical University/Suzhou Municipal Hospital, Jiangsu Suzhou 215104, China
关键词:
间接法; 高尔基体蛋白73; 成人; 参考区间; 苏州地区
分类号:
R446.11
DOI:
10.3969/j.issn.1671-7414.2026.01.031
文献标志码:
A
摘要:
目的采用间接法建立并验证苏州地区表观健康成人血清中高尔基体蛋白73（GP73）的参考区间。方法回顾性分析2020年5月～2024年4月在南京医科大学附属苏州医院健康体检的1959例受试者，采用酶联免疫吸附试验（ELISA）检测血清中GP73水平。按照性别和年龄分组，参照WS/T402-2024《临床实验室定量检验项目参考区间的制定》文件要求建立各组参考区间。选取2024年5月～6月该院20例表观健康成人，进行GP73自建参考区间的验证。结果表观健康人GP73水平呈偏态分布，采用百分位数法建立参考区间。线性回归分析发现，男性和女性GP73水平与年龄均呈正相关(r=0.197、0.247，均P<0.01)。男性组GP73水平低于女性组，差异具有统计学意义（Z=-2.145，P=0.032）。按年龄分组，≥60岁组与18～44岁组、45～59岁组GP73水平比较，差异均有统计学意义（Z=-6.623、-6.625，均P<0.05），18～44岁组与45～59岁组GP73水平比较，差异无统计学意义（Z=-0.232，P=0.817）。女性不同年龄分组两两比较，GP73水平差异均有统计学意义（Z=-2.371、-7.571、-4.907，均P<0.05）。经过标准正态离差检验分析不同性别和各年龄段GP73水平差异无实际临床意义（Z<Z*），建立统一的GP73参考区间为≤76.96ng/ml，并进行验证符合标准。结论初步建立适合苏州地区表观健康成人血清GP73参考区间，为临床相关疾病的预防、诊治提供参考。
Abstract:
Objective To establish and validate reference interval of serum Golgi protein 73(GP73) in heathy adults in Suzhou area by indirect method. Methods Retrospective analysis was conducted on 1 959 subjects who underwent health examinations at the Affiliated Suzhou Hospital of Nanjing Medical University from May 2020 to April 2024.The serum GP73 level was de-tected by enzyme-linked immunosorbent assay (ELISA). The patients were divided into groups according to gender and age. The reference intervals for each group were established according to WS/T 402-2024 defining, establishing, and verifying reference intervals of quantitative analytes in the clinical laboratory document.Twenty apparently healthy subjects from the same hospital from May to June 2024 were selected to validate the self-constructed reference interval of GP73. Results The levels of GP73 in apparently healthy people were skewed, and the reference intervals were established using the percentile method. Linear regres-sion analysis showed that GP73 levels was positively correlated with age in both males and females (r=0.197, 0.247, all P<0.01). The GP73 levels in the male group was significantly lower than that in the female group (Z=-2.145, P=0.032).The GP73 levels in the male groups ≥ 60 years were significantly different from those in the 18~44 years and 45~59 years groups （Z=-6.623、-6.625，all P<0.05），However, no significant difference in GP73 levels was found between the 18~44 years and 45~59 years groups （Z=-0.232, P=0.817）.In females, pairwise comparisons between different age groups showed significant differences in GP73 lev-els （Z=-2.371, -7.571, -4.907, all P<0.05）. The analysis of Z test results indicated that the differences in GP73 levels between genders and age groups held no actual clinical significance (Z < Z*), thus a unified reference interval for GP73 was established as≤76.96 ng/ml. Conclusions The preliminary reference range for serum GP73 in apparently healthy adults in Suzhou area has been established, providing reference for the prevention, diagnosis and treatment of clinically related diseases.

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备注/Memo

备注/Memo:
基金项目：南京医科大学科技发展基金一般项目（NMUB20220184）。
作者简介：李淑湘(1987-)，女，硕士，副主任技师，研究方向：临床免疫学，E-mail：jsszlishuxiang@126.com。
通讯作者：魏衍财(1986-)，男，硕士，副主任技师，研究方向：临床实验室质量控制。
更新日期/Last Update: 2026-01-15