[1]刘 旺,岳黎明,曹 蛟,等.麦冬皂苷对脓毒血症并发急性呼吸窘迫综合征小鼠肺屏障功能的影响及机制研究[J].现代检验医学杂志,2026,41(02):83-87.[doi:10.3969/j.issn.1671-7414.2026.02.014]
　LIU Wang,YUE Liming,CAO Jiao,et al.Study on the Effect of Ophiopogon Japonicus Saponins on Lung Barrier Function in Mice with Sepsis Associated Acute Respiratory Distress Syndrome[J].Journal of Modern Laboratory Medicine,2026,41(02):83-87.[doi:10.3969/j.issn.1671-7414.2026.02.014]
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麦冬皂苷对脓毒血症并发急性呼吸窘迫综合征小鼠肺屏障功能的影响及机制研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
83-87
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Study on the Effect of Ophiopogon Japonicus Saponins on Lung Barrier Function in Mice with Sepsis Associated Acute Respiratory Distress Syndrome
文章编号:
1671-7414（2026）02-083-05
作者:
刘 旺1，岳黎明1，曹 蛟1，胡彦伟1，敖 铁2
1.陕西中医药大学附属医院重症医学科，陕西咸阳 712000;2.西安国际医学中心医院呼吸与危重症医学科，西安 710100
Author(s):
LIU Wang1，YUE Liming1，CAO Jiao1，HU Yanwei1，AO Tie2
1.Department of Intensive Care Medicine, Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Shaanxi Xianyang 712000, China;2.Department of Respiratory and Critical Care Medicine, Xi’an International Medical Center Hospital, Xi’an 710100, China
关键词:
麦冬皂苷; 脓毒症并发急性呼吸窘迫综合征; 肺屏障功能; 细胞通讯网络因子1信号通路
分类号:
R515.3；R563.8；R-332
DOI:
10.3969/j.issn.1671-7414.2026.02.014
文献标志码:
A
摘要:
目的探究麦冬皂苷对脓毒血症并发急性呼吸窘迫综合征(ARDS)小鼠肺屏障功能的影响及作用机制。方法选择A549细胞进行脂多糖(LPS)造模培养,分为对照组、模型组(LPS诱导)、麦冬皂苷低剂量组(100?g/ml)、高剂量组(200?g/ml)。使用CCK-8实验观察细胞活性。通过腹腔注射LPS构建脓毒血症ARDS小鼠模型。将40只SPF级雄性小鼠随机分为正常对照组、脓毒血症ARDS模型组、脓毒血症ARDS+低剂量麦冬皂苷治疗组、脓毒血症ARDS+高剂量麦冬皂苷治疗组,每组10只。定期观察小鼠体重、呼吸频率、精神状态等评估疾病严重程度。荧光分光光度计检测肺组织通透性。HE染色观察组织病理形态,ELISA检测炎症因子表达,Westernblot检测紧密连接蛋白中的闭锁蛋白(Occludin)和细胞通讯网络因子1(CCN1)表达。结果与对照组相比,模型组A549细胞活性降低(t=37.900,P<0.001)。与模型组相比,低剂量组与高剂量组A549细胞活性上升(t=12.730、27.720,均P<0.001)。与对照组相比,模型组DAI评分升高;与模型组相比,麦冬皂苷治疗组(低剂量及高剂量)的评分逐渐降低(F=423.678~1057.870,均P<0.001)。与对照组相比,模型组的肺组织炎症细胞浸润和通透性增加;与模型组相比,麦冬皂苷治疗组肺组织炎症细胞浸润减少,肺组织通透性降低。与对照组相比,模型组肺组织TNF-α、IL-6表达水平升高(t=36.100、32.330,均P<0.001);与模型组相比,低剂量组和高剂量组肺组织里的TNF-α、IL-6表达水平降低(t=19.610~33.380,均P<0.001)。与对照组相比,模型组CCN1表达量增加;与模型组相比,低剂量组和高剂量组CCN1表达量降低(F=178.284,P<0.01)。与对照组相比,模型组Occludin表达量降低;与模型组相比,低剂量组和高剂量组Occludin表达量升高(F=99.807,P<0.001)。结论麦冬皂苷通过调节CCN1信号通路,改善脓毒血症ARDS小鼠肺屏障功能,减轻炎症反应,为其治疗提供新靶点。
Abstract:
Objective To investigate the effect and mechanism of Ophiopogon japonicus saponins on lung barrier function in mice with sepsis associated acute respiratory distress syndrome (SARDS). Methods A549 cells were cultured and modeled with lipo-polysaccharide (LPS) stimulation, and divided into the following groups: control group, model group (LPS-induced), low-dose ophiopogon saponin group (100 ?g/ml) and high-dose group (200 ?g/ml). The CCK-8 assay was used to assess cell viability. A mouse model of sepsis-induced ARDS was established via intraperitoneal injection of LPS. Forty male SPF-grade mice were ran-domly divided into four groups: normal control group, sepsis-induced ARDS model group, sepsis-induced ARDS + low-dose ophiopogon saponin treatment group and sepsis-induced ARDS + high-dose ophiopogon saponin treatment group, with 10 mice in each group. Disease severity was evaluated by regularly monitoring body weight, respiratory rate and mental state. Lung tissue permeability was measured using a fluorescence spectrophotometer. Histopathological morphology was observed via HE stain-ing. Inflammatory factor expression was assessed using ELISA, and Western blotting was used to detect the expression of tight junction protein occludin and cellular communication network factor 1 (CCN1) Results Compared with the control group, the viability of A549 cells was significantly reduced in the model group (t=37.900, P<0.001). Compared with the model group, cell viability increased in both the low-dose and high-dose ophiopogon saponin groups (t=12.730, 27.720, P<0.001). Compared with the control group, the DAI score was significantly higher in the model group. In contrast, the scores in the ophiopogon sapo-nin treatment groups (low-dose and high-dose) gradually decreased compared with the model group (F=423.678~1 057.870, all P<0.001). In the model group, lung tissue inflammation and permeability were significantly increased compared with the control group. However, lung tissue inflammation and permeability were reduced in the ophiopogon saponin treatment groups compared with the model group. The expression levels of TNF-α and IL-6 in lung tissue were significantly higher in the model group com-pared with the control group (t=36.100, 32.330, all P<0.001). These levels were significantly decreased in both the low-dose and high-dose groups compared with the model group (t=19.610~33.380, all P<0.001). Compared with the control group, the expres-sion of CCN1 was increased in the model group, while it was decreased in both the low-dose and high-dose treatment groups compared with the model group (F=178.284, P<0.01). Similarly, the expression of Occludin was reduced in the model group compared with the control group, but was elevated in the low-dose and high-dose treatment groups compared with the model group (F=99.807, P<0.001). Conclusions Ophiopogon japonicus saponins improve pulmonary barrier function and alleviate in-flammation in sepsis-associated ARDS mice by modulating the CCN1 signaling pathway, providing a new therapeutic target for the treatment of this condition.

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备注/Memo

备注/Memo:
基金项目：陕西省卫生健康委科研课题（编号：2024PT012）。
作者简介：刘旺（1992-），男，本科，主治医师，研究方向：中医药防治脓毒症、ARDS， E-mail：fyoma08@163.com。
通讯作者：敖铁（1991-），男，硕士，主治医师，研究方向：呼吸与危重症医学疾病诊治，E-mail：bajia5021@163.com。
更新日期/Last Update: 2026-03-15