[1]徐 鹏,彭 继,谢 锋,等.干姜附子水煎液调控Nrf2/HO-1介导的铁死亡抑制大鼠心肌缺血再灌注损伤模型的实验研究[J].现代检验医学杂志,2026,41(02):95-99.[doi:10.3969/j.issn.1671-7414.2026.02.016]
　XU Peng,PENG Ji,XIE Feng,et al.Experimental Study on the Regulation of Nrf2/HO-1 Mediated Ferroptosis by Ganjiang Fuzi Water Decoction on Rat Myocardial Ischemia-Reperfusion Injury Model[J].Journal of Modern Laboratory Medicine,2026,41(02):95-99.[doi:10.3969/j.issn.1671-7414.2026.02.016]
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干姜附子水煎液调控Nrf2/HO-1介导的铁死亡抑制大鼠心肌缺血再灌注损伤模型的实验研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
95-99
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Experimental Study on the Regulation of Nrf2/HO-1 Mediated Ferroptosis by Ganjiang Fuzi Water Decoction on Rat Myocardial Ischemia-Reperfusion Injury Model
文章编号:
1671-7414（2026）02-095-05
作者:
徐 鹏1，彭 继1，谢 锋1,2，黄九林2
1.安康市中医医院，陕西安康 725000;2.安康学院秦巴中药资源研发中心，陕西高校青年创新团队，陕西安康 725000
Author(s):
XU Peng1，PENG Ji1，XIE Feng1,2，HUANG Jiulin2
1.Ankang Hospital of Traditional Chinese Medicine, Shaanxi Ankang 725000, China;2.Ankang University, Qinba Chinese Medicine Resources R&D Center, the Youth Innovation Team of Shaanxi Universities, Shaanxi Ankang 725000, China
关键词:
干姜附子水煎液; 核因子E2相关因子2/血红素加氧酶-1; 心肌缺血再灌注; 铁死亡
分类号:
R-332；R446
DOI:
10.3969/j.issn.1671-7414.2026.02.016
文献标志码:
A
摘要:
目的?观察干姜附子水煎液调控核因子E2相关因子(Nrf2)/血红素加氧酶-1(HO-1)介导的铁死亡对大鼠心肌缺血再灌注损伤(MI/RI)的影响及其机制。方法将50只大鼠随机分为空白组、模型组、干姜附子水煎液低、高剂量组和Nrf2抑制剂(ML385)组,每组10只。建立MI/RI大鼠模型,采用2,3,5氯化三苯基四氯唑(TTC)染色分析各组大鼠心肌梗死面积百分比,酶联免疫吸附试验(ELISA)检测各组大鼠血清心肌损伤标志物的水平、心肌组织活性氧(ROS)水平,比色法检测各组大鼠心肌组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)、谷胱甘肽(GSH)和亚铁离子(Fe2+)含量。通过蛋白质印迹(WesternBlot)检测各组大鼠心肌组织中Nrf2、HO-1蛋白表达。结果与空白组大鼠相比,MI/RI模型组大鼠的心肌梗死面积百分比显著升高,血清中cTnI、CK-MB和LDH水平显著增加,心肌组织中ROS水平、MDA和Fe2+含量显著升高,SOD活性和GSH含量显著降低,Nrf2、HO-1蛋白表达显著降低,差异具有统计学意义(t=4.359~17.701,均P<0.001)。与MI/RI模型组相比,干姜附子水煎液低、高剂量组和ML385组大鼠的心肌梗死面积百分比显著降低,血清cTnI、CK-MB和LDH水平显著下降,心肌组织中ROS水平、MDA和Fe2+含量显著降低,SOD活性和GSH含量显著升高,Nrf2、HO-1蛋白表达水平显著升高,差异具有统计学意义(t低剂量组=2.382~8.293;t高剂量组=2.476~7.474;tML385组=2.476~7.580,均P<0.001)。与干姜附子水煎液高剂量组相比,ML385组大鼠的心肌梗死面积百分比显著升高,血清cTnI、CK-MB和LDH水平显著升高,心肌组织中ROS、MDA和Fe2+含量显著升高,SOD活性和GSH含量显著降低,Nrf2、HO-1蛋白表达水平显著降低,差异具有统计学意义(t=5.918~13.139,均P<0.001)。结论干姜附子水煎液通过调控Nrf2/HO-1通路介导的铁死亡抑制大鼠MI/RI,且其作用呈剂量依赖性。
Abstract:
Objective Observation of the effect and mechanism of water decoction of Ganjiang Fuzi on ferroptosis mediated by nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) in rats with myocardial ischemia / reperfusion injury (MI/RI). Methods 50 rats were randomly divided into the blank group, model group, low or high dose groups of water decoction of Ganjiang Fuzi, and an Nrf2 inhibitor (ML385) group, with 10 rats in each group. MI/RI rat models were established. TTC stain-ing was used to analyze the percentage of myocardial infarction area. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of serum myocardial injury markers and myocardial tissue reactive oxygen species (ROS). Colorimetric method was used to detect the activity of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) and ferrous ion (Fe2+) content. Western blot was used to detect Nrf2 and HO-1 protein expression in myocardial tissue of rats in each group. Results Compared with the blank group, the MI/RI model group showed significantly increased myocardial infarction area percentage, serum cTnI, CK-MB and LDH levels, and myocardial tissue ROS level, MDA and Fe2+ content, while exhibited significantly reduced SOD activity, GSH content, and expression of Nrf2 and HO-1 proteins (t=4.359~17.701, all P<0.001). Compared with the MI/RI model group, low or highdose groups of water decoction of Ganjiang Fuzi and ML385 group showed significantly re-duced myocardial infarction area percentage, serum cTnI, CK-MB and LDH levels, myocardial tissue ROS levels, MDA and Fe2+content, and significantly elevated SOD activity, GSH content, and Nrf2 and HO-1 proteins expression levels (t low dose groups of water de-coction of Ganjiang Fuzi =2.382~8.293;t high dose groups of water decoction of Ganjiang Fuzi =2.476~7.474;tML385 groups =2.476~7.580,all P<0.001). Com-pared with the high dose group of water decoction of Ganjiang Fuzi, the ML385 group exhibited significantly increased myocar-dial infarction area percentage, serum cTnI/CK-MB and LDH levels, myocardial tissue ROS, MDA and Fe2+, and significantly reduced SOD activity and GSH content, and Nrf2 and HO-1 proteins expression levels (t=5.918~13.139, all P<0.001). Conclu-sions The water decoction of Ganjiang Fuzi inhibits MI/RI in rats by regulating the Nrf2/HO-1 pathway mediated ferroptosis in a dose-dependent manner.

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备注/Memo

备注/Memo:
基金项目：陕西省科协青年人才项目(20240367)。
作者简介：徐鹏(1991-)，男，本科，主管药师，研究方向：药物制剂、药学，E-mail：546855011@qq.com。
通讯作者：彭继(1993-)，男，本科，主管药师，研究方向：药学、药理学，E-mail：531342621@qq.com。
更新日期/Last Update: 2026-03-15