[1]郑 红,李 磊,张文杰,等.新生儿支气管肺发育不良患者血清miR-382-5p、miR-203a-3p水平表达及临床意义[J].现代检验医学杂志,2026,41(02):117-121.[doi:10.3969/j.issn.1671-7414.2026.02.020]
　ZHENG Hong,LI Lei,ZHANG Wenjie,et al.Serum miR-382-5p and miR-203a-3p Levels and Their Clinical Significance in Patients with Neonatal Bronchopulmonary Dysplasia[J].Journal of Modern Laboratory Medicine,2026,41(02):117-121.[doi:10.3969/j.issn.1671-7414.2026.02.020]
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新生儿支气管肺发育不良患者血清miR-382-5p、miR-203a-3p水平表达及临床意义()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
117-121
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Serum miR-382-5p and miR-203a-3p Levels and Their Clinical Significance in Patients with Neonatal Bronchopulmonary Dysplasia
文章编号:
1671-7414（2026）02-117-05
作者:
郑 红，李 磊，张文杰，张婷婷
淮安市妇幼保健院新生儿科，江苏淮安 223002
Author(s):
ZHENG Hong，LI Lei，ZHANG Wenjie，ZHANG Tingting
Department of Neonatology, Huai’an Maternal and Child Health Hospital, Jiangsu Huai’an 223002, China
关键词:
新生儿支气管肺发育不良; 微小核糖核酸-382-5p; 微小核糖核酸-203a-3p
分类号:
R722.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2026.02.020
文献标志码:
A
摘要:
目的探究新生儿支气管肺发育不良(BPD)患儿血清微小RNA(miR)-382-5p、miR-203a-3p水平变化及意义。方法选取2021年1月~2024年1月在淮安市妇幼保健院出生的BPD患儿130例作为研究对象纳入观察组,另选取同期足月伴有呼吸障碍新生儿125例作为对照组。实时荧光定量PCR(RT-qPCR)法检测所有新生儿血清miR-382-5p、miR-203a-3p水平,采用Pearson法分析BPD患儿血清miR-382-5p、miR-203a-3p表达水平的相关性,采用Logistic回归分析新生儿患BPD的相关因素,采用受试者操作特征(ROC)曲线分析血清miR-382-5p、miR-203a-3p水平对BPD发生的预测价值。结果观察组患儿出现新生儿窒息、需要机械通气人数占比、氧疗时间、呼出气一氧化氮(FeNO)、血清miR-203a-3p水平显著高于对照组(t/χ2=9.046~23.234),血清miR-382-5p水平显著低于对照组(t=8.785),差异具有统计学意义(均P<0.05)。血清miR-382-5p与miR-203a-3p表达水平呈负相关(r=-0.404,P<0.001);高水平血清miR-382-5p是患BDP的独立保护因素,高水平血清miR-203a-3p是患BDP的独立危险因素(Waldχ2=7.101、7.858,均P<0.05);患儿血清miR-382-5p、miR-203a-3p二者联合预测BDP发生的曲线下面积(AUC)优于miR-382-5p、miR-203a-3p单独预测,差异具有统计学意义(Z=2.023、2.119,均P<0.05)。结论BDP患儿血清miR-382-5p水平降低,miR-203a-3p水平升高,二者联合检测可有效预测新生儿BPD的发生。
Abstract:
Objective To investigate the changes and clinical significance of serum miR-382-5p and miR-203a-3p levels in patients with neonatal bronchopulmonary dysplasia (BPD). Methods A total of 130 neonates with BPD born at Huai’an Maternal and Child Health Hospital between January 2021 and January 2024 were enrolled as the study subjects and labeled as observation group. An additional 125 full-term neonates with respiratory distress born during the same period were labeled as control group. Using U6 as an internal reference gene, fluorescence quantitative PCR (RT-qPCR) was used to detect the levels of miR-382-5p and miR-203a-3p in the serum of all neonates. Pearson correlation analysis was performed to assess the correlation between serum miR-382-5p and miR-203a-3p expression levels in BPD neonates. Logistic regression analysis identified factors associated with BPD development. In addition, receiver operating characteristic (ROC) curve was used to analyze the predictive value of serum miR-382-5p and miR-203a-3p for the occurrence of BPD. Results The incidence of neonatal asphyxia and proportions requiring mechanical ventilation, duration of oxygen therapy, exhaled nitric oxide (FeNO),and serum miR-203a-3p in the observation group were significantly higher than those of control group (t/χ2=9.046~23.234), while serum miR-382-5p levels were significantly lower than those in the control group (t=8.785), and the differences were statistically significant (all P<0.05). The expression levels of serum miR-382-5p and miR-203a-3p in neonates with BDP were negatively correlated (r=-0.404, P<0.001). Elevated serum miR-382-5p was an independent protective factor against BDP (P<0.05), while elevated serum miR-203a-3p acted as an independent risk factor for BDP (Wald χ2=7.101, 7.858, all P<0.05). The combined prediction of BPD using serum miR-382-5p and miR-203a-3p demonstrated superior area under the curve (AUC) compared to prediction with either miR-382-5p or miR-203a-3p alone, with statistically significant differenc-es (Z=2.023, 2.119, all P<0.05). Conclusions Compared with healthy neonates, the serum miR-382-5p is reduced and miR-203a-3p is increased in neonates with BPD. The changes in both levels can effectively predict the occurrence of neonatal BPD.

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备注/Memo

备注/Memo:
基金项目：江苏省卫生健康委员会科研项目（编号：H1018009）。
作者简介：郑红（1989-），女，硕士研究生，主治医师，研究方向：支气管肺发育不良，E-mail：1160209164@qq.com。
通讯作者：张婷婷（1990-），女，硕士研究生，主治医师，研究方向：支气管肺发育不良，E-mail：1047718202@qq.com。
更新日期/Last Update: 2026-03-15