[1]赵晓霞,仇付会,刘腾飞,等.艾滋病患者血清sCD14、CGRP、FOXO3水平表达与疾病分期和免疫功能的相关性研究[J].现代检验医学杂志,2026,41(02):154-159+173.[doi:10.3969/j.issn.1671-7414.2026.02.026]
　ZHAO Xiaoxia,QIU Fuhui,LIU Tengfei,et al.Study on the Correlation between the Expression Levels of Serum sCD14, CGRP and FOXO3 in AIDS Patients and Disease Stage and Immune Function[J].Journal of Modern Laboratory Medicine,2026,41(02):154-159+173.[doi:10.3969/j.issn.1671-7414.2026.02.026]
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艾滋病患者血清sCD14、CGRP、FOXO3水平表达与疾病分期和免疫功能的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
154-159+173
栏目:
出版日期:
2026-03-15

文章信息/Info

Title:
Study on the Correlation between the Expression Levels of Serum sCD14, CGRP and FOXO3 in AIDS Patients and Disease Stage and Immune Function
文章编号:
1671-7414（2026）02-154-07
作者:
赵晓霞1，仇付会2，刘腾飞2，张 志2
1.石家庄市妇幼保健院检验科，石家庄 050000;2.石家庄市第五医院检验科，石家庄 050000
Author(s):
ZHAO Xiaoxia1，QIU Fuhui2，LIU Tengfei2，ZHANG Zhi2
1.Department of Clinical Laboratory, Shijiazhuang Maternal and Child Health Hospital, Shijiazhuang 050000, China;2.Department of Clinical Laboratory, Shijiazhuang Fifth Hospital, Shijiazhuang 050000, China
关键词:
艾滋病; 可溶性白细胞分化抗原14; 神经元降钙素基因相关肽; 叉头转录因子O亚型3; 疾病分期; 免疫功能
分类号:
R512.91；R446.62
DOI:
10.3969/j.issn.1671-7414.2026.02.026
文献标志码:
A
摘要:
目的 分析艾滋病(AIDS)患者血清可溶性白细胞分化抗原14(sCD14)、神经元降钙素基因相关肽(CGRP)、叉头转录因子O亚型3(FOXO3)水平与疾病分期和免疫功能的关系。方法选取2021年1月~2024年1月石家庄市第五医院和石家庄市妇幼保健院收治的175例AIDS患者及175例健康体检者为研究对象,分别纳入AIDS组和对照组,采用酶联免疫吸附法(ELISA)检测两组受试者血清sCD14、CGRP和FOXO3表达水平。采用Spearman法和Pearson法分析sCD14、CGRP、FOXO3水平与疾病分期和免疫功能的相关性,采用多因素Logistic分析AIDS患者预后不良的影响因素,采用受试者工作特征(ROC)曲线预测sCD14、CGRP和FOXO3水平对AIDS患者预后不良的预测价值。结果与对照组比较,AIDS组患者血清sCD14(5.12±1.25μg/mlvs2.83±0.46μg/ml)、CD8(+476.88±51.38个/μlvs396.57±35.42个/μl)水平显著升高;CGRP(42.38±14.69pg/mlvs76.53±18.54pg/ml)、FOXO3(5.81±1.34ng/mlvs8.02±1.07ng/ml)、CD4+(271.18±68.54个/μlvs728.69±88.49个/μl)水平及CD4+/CD8(+0.57±0.12vs1.20±0.15)比值显著降低,差异具有统计学意义(t=17.049~54.072,均P<0.05)。AIDS组患者血清sCD14、CD8+水平在急性期组、无症状期组、艾滋病期组依次升高,CGRP、FOXO3、CD4+水平及CD4+/CD8+比值依次下降,差异具有统计学意义(F=25.322~125.502,均P<0.05)。sCD14与CD4+、CD4+/CD8+呈负相关,与CD8+、疾病分期呈正相关;CGRP、FOXO3与CD4+、CD4+/CD8+呈正相关,与CD8+、疾病分期呈负相关,差异具有统计学意义(r=-0.746~0.759,均P<0.05)。sCD14、CGRP、FOXO3水平与生殖器疱疹、淋巴瘤及HIV载量有关,差异具有统计学意义(t/F=2.381~39.190,均P<0.05)。预后不良组患者血清sCD14、CD8+水平及HIV载量高人数占比显著高于预后良好组,CGRP、FOXO3、CD4+水平及CD4+/CD8+比值显著低于预后良好组,差异具有统计学意义(t=5.071~10.577,均P<0.05)。sCD14、CD8+及HIV载量高为AIDS患者预后不良的危险因素,CGRP、FOXO3、CD4+及CD4+/CD8+为AIDS患者预后不良的保护因素,差异具有统计学意义(Waldχ2=6.349~18.825,均P<0.05)。sCD14、CGRP、FOXO3水平联合预测AIDS患者预后不良的AUC高于各项单独预测的AUC,差异具有统计学意义(Z=3.757、3.836、3.353,均P<0.05)。结论sCD14在AIDS患者血清中上调表达,CGRP、FOXO3下调表达,与疾病分期和免疫功能关系密切,三者联合预测AIDS患者预后不良价值较高。
Abstract:
Objective To investigate the relationship between the levels of serum soluble cluster of differentiation antigen 14 (sCD14), calcitonin gene related peptide (CGRP), forkhead box transcription protein 3 (FOXO3) with disease stage and immune function in acquired immune deficiency syndromes (AIDS) patients. Methods 175 AIDS patients admitted to and 175 healthy individuals under going physical examinations in Shijiazhuang Fifth Hospital and Shijiazhuang Maternal and Child Health Hos-pital from January 2021 to January 2024 were enrolled as the AIDS group and the control group, respectively. The enzyme-linked immunosorbent assay (ELISA) method was applied to detect the expression levels of sCD14, CGRP and FOXO3 in the serum of both groups. Spearman and Pearson correlation analyses were applied to analyze the correlation between sCD14, CGRP, FOXO3 levels with disease stage and immune function. Multivariate Logistic regression was used to analyze the factors influencing poor prognosis in patients with AIDS. Receiver operating characteristic (ROC) curve was applied to predict the predictive value of sCD14, CGRP and FOXO3 levels for poor prognosis in AIDS patients. Results Compared with the control group, the levels of serum sCD14 (5.12±1.25 μg/ml vs 2.83±0.46 μg/ml), and CD8+ (476.88±51.38 cells/μl vs 396.57±35.42 cells/μl) were significantly increased, while the levels of CGRP (42.38±14.69 pg/ml vs 76.53±18.54 pg/ml), FOXO3 (5.81±1.34 ng/ml vs 8.02±1.07 ng/ml), CD4+ (271.18±68.54 cells/μl vs 728.69±88.49 cells/μl) and the ratio of CD4+/CD8+ (0.57±0.12 vs 1.20±0.15) were significantly decreased (t=17.049~54.072, all P<0.05) in the AIDS group. The serum levels of sCD14 and CD8+ in AIDS group progressively increased across the acute phase, asymptomatic phase and AIDS phase, while the levels of CGRP, FOXO3, CD4+ and the ratio of CD4+/CD8+ progressively decreased, with statistically significant differences (F=25.322~125.502, all P<0.05). The sCD14 was negatively correlated with CD4+ and CD4+/CD8+, and positively correlated with CD8+ and disease stage . CGRP and FOXO3 were positively correlated with CD4+ and CD4+/CD8+, and negatively correlated with CD8+ and disease stage , with statistically significant differences (r=-0.746~0.759, all P<0.05). The levels of sCD14, CGRP, and FOXO3 were significantly associated with genital herpes, lymphoma, and HIV viral load (t/F = 2.381~39.190, all P<0.05). The levels of serum sCD14, CD8+ and HIV viral load in the poor prognosis group were significantly higher than those in the fa-vorable prognosis group. Conversely, the levels of CGRP, FOXO3, CD4+ and the CD4+/CD8+ ratio were significantly lower in the poor prognosis group than those in the favorable prognosis group, with statistically (t=5.071~10.577, all P<0.05). Elevated sCD14, CD8+ and HIV viral load were risk factors, while CGRP, FOXO3, CD4+ and CD4+/CD8+ ration were protective factors for poor prognosis in AIDS patients, with statistical significances (Wald χ2 = 6.349~18.825, all P<0.05). The combined AUC for predicting poor prognosis in AIDS patients using sCD14, CGRP, and FOXO3 levels was higher than the AUC for predicting poor prognosis using sCD14 , CGRP, or FOXO3 alone significant differences (Z=3.757, 3.836, 3.353, all P<0.05). Conclusions sCD14 is upregulated in the serum of AIDS patients, while CGRP and FOXO3 are downregulated, these markers are closely re-lated to disease stage and immune function, and their combined use demonstrates high predictive value for poor prognosis in AIDS patients.

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备注/Memo

备注/Memo:
基金项目：石家庄市科技计划自筹资金项目（231460223)。
作者简介：赵晓霞（1989-），女，本科，副主任技师，研究方向：艾滋病人免疫状态实验室检测的相关研究，E-mail：15032703958@sina.cn。
通讯作者：张志（1980-），男，硕士，副主任技师，研究方向：细菌耐药基因的相关研究。
更新日期/Last Update: 2026-03-15