[1]刘远山,乔健健,张 涛,等.2023~2024年江苏宜兴地区99株沙门氏菌血清学分型与全基因组学特征分析研究[J].现代检验医学杂志,2026,41(03):80-86.[doi:10.3969/j.issn.1671-7414.2026.03.015]
　LIU Yuanshan,QIAO Jianjian,ZHANG Tao,et al.A Study on the Serotyping and Whole-Genome Characteristics of 99 Salmonella Strains Collected in Yixing, Jiangsu Province (2023~2024)[J].Journal of Modern Laboratory Medicine,2026,41(03):80-86.[doi:10.3969/j.issn.1671-7414.2026.03.015]
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2023~2024年江苏宜兴地区99株沙门氏菌血清学分型与全基因组学特征分析研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年03期
页码:
80-86
栏目:
论著
出版日期:
2026-05-13

文章信息/Info

Title:
A Study on the Serotyping and Whole-Genome Characteristics of 99 Salmonella Strains Collected in Yixing, Jiangsu Province (2023~2024)
文章编号:
1671-7414（2026）03-080-07
作者:
刘远山，乔健健，张 涛，吴亦琴
宜兴市疾病预防控制中心，江苏宜兴 214206
Author(s):
LIU Yuanshan，QIAO Jianjian，ZHANG Tao，WU Yiqin
Yixing Center for Disease Control and Prevention, Jiangsu Yixing 214206, China
关键词:
沙门氏菌; 血清学分型; 全基因组学; 宜兴地区
分类号:
R378.22；R446.5
DOI:
10.3969/j.issn.1671-7414.2026.03.015
文献标志码:
A
摘要:
目的?分析江苏宜兴地区沙门氏菌的分子流行病学特征及抗生素耐药情况，为区域性沙门氏菌防控提供科学依据。方法?对宜兴市2023~2024年食源性疾病患者分离的48株沙门氏菌和健康从业人员体检中分离的51株沙门氏菌进行血清学分型、全基因组测序和药物敏感性试验，分析菌株的血清学分型、多位点序列分型、耐药基因、毒力基因、系统发育关系、耐药表型。结果在99株沙门氏菌中共检出25种血清型和21种ST型，鼠伤寒沙门氏菌占比最高（42.42%），其在腹泻患者分离株中占比达56.25%。优势ST型为ST19（26.26%）、ST34（19.19%）和ST11（14.14%），ST19型在腹泻患者分离株中占比最高（37.50%）。共检测出28种耐药基因，20种耐药基因检出率为100%，耐药基因TEM-1在腹泻患者分离株中检出率最高（75.00%），差异具有统计学意义（χ2=15.72，P<0.001）。毒力基因共检出107种，79种毒力基因检出率为100%，sodCI和sseI/srfH毒力基因在腹泻患者分离株中检出率显著（77.08%），差异具有统计学意义（χ2=4.55，P=0.036）。耐药基因TEM-1、tet(B)、AAC(6’)-Iaa与多种毒力基因具有显著相关性。系统发育分析显示，99株沙门氏菌共分为6个主要分支，其中分支I菌株毒力基因数量最高（106.96±0.20），腹泻患者分离株的占比也最大（68.00%）。耐药分析显示，沙门氏菌分离株对氨苄西林（65.66%）和四环素（59.60%）具有高耐药性。结论宜兴地区沙门氏菌血清型、ST型、耐药表型种类多样，具有一定的多重耐药性，且呈现耐药与毒力基因协同的特征，应注意抗生素使用并进行耐药与毒力基因的协同监测。
Abstract:
Objective To analyze the molecular epidemiological characteristics and antibiotic resistance profiles of Salmonella isolates in the Yixing region of Jiangsu Province, providing a scientific basis for regional Salmonella control and prevention. Methods Serotyping, whole-genome sequencing, and antimicrobial susceptibility testing were performed on 48 Salmonella strains isolated from patients with foodborne illnesses and 51 strains isolated from healthy food industry workers during physical examinations in Yixing City from 2023 to 2024. The analyses focused on serotyping, multilocus sequence typing (MLST), antibi-otic resistance genes, virulence genes, phylogenetic relationships, and resistance phenotypes. Results Among the 99 Salmonella strains, 25 serotypes and 21 sequence types (ST) were identified. Salmonella typhimurium accounted for the highest proportion (42.42%), representing 56.25% of the isolates from diarrheal patients. The predominant ST types were ST19 (26.26%), ST34 (19.19%), and ST11 (14.14%), with ST19 being the most prevalent among isolates from diarrheal patients (37.50%). A total of 28 antimicrobial resistance genes were detected, 20 of which were present in all isolates. The TEM-1 gene showed a significantly higher prevalence in isolates from diarrheal patients (75.00%, χ2=15.72, P<0.001). A total of 107 virulence genes were identified, with 79 genes present in all isolates. The detection rates of sodCI and sseI/srfH genes were significantly higher in isolates from diarrheal patients (77.08%, χ2=4.55, P=0.036). Correlation analysis revealed significant positive associations between the resis-tance genes TEM-1, tet(B), AAC(6’)-Iaa, and multiple virulence genes. Phylogenetic analysis classified the 99 isolates into six major clades, with clade I having the highest number of virulence genes (106.96±0.20) and the greatest proportion of diarrheal patient isolates (68.00%). Antibiotic resistance analysis revealed that Salmonella isolates exhibited high resistance to Ampicil-lin (65.66%) and Tetracycline (59.60%). Conclusions Salmonella isolates in the Yixing region exhibit diverse serotypes, ST types, and resistance phenotypes, with a certain degree of multidrug resistance, and display characteristics of synergy between resistance and virulence genes, showing the importance of prudent antibiotic use and the coordinated surveillance of both resis-tance and virulence genes.

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相似文献/References:

[1]王　维,王　林.2018～2020年西安地区儿童食源性腹泻病病原菌分布特征和耐药性及鼠伤寒沙门菌聚类分析[J].现代检验医学杂志,2021,36(04):51.[doi:10.3969/j.issn.1671-7414.2021.04.011]
　WANG Wei,WANG Lin.Analysis of Pathogen Distribution and Drug Resistance of Food-BorneDiarrhea in Children of Xi’an Area from 2018 to 2020[J].Journal of Modern Laboratory Medicine,2021,36(03):51.[doi:10.3969/j.issn.1671-7414.2021.04.011]

备注/Memo

备注/Memo:
基金项目：宜兴市“陶都之光”科技攻关项目（2023SF25）。
作者简介：刘远山（1995-），男，博士，实验师，研究方向：实验室检验分析，E-mail：936042873@qq.com。
通讯作者：吴亦琴，女，硕士，副主任技师，研究方向：微生物检验，E-mail：363221168@qq.com。
更新日期/Last Update: 2026-05-15