[1]侯青春,毛伟鉴,王益夫,等.三七总皂苷对小鼠腹主动脉瘤模型血管平滑肌细胞表型转换及MMPs/TIMPs平衡影响的实验研究[J].现代检验医学杂志,2026,41(03):177-181.[doi:10.3969/j.issn.1671-7414.2026.03.032]
　HOU Qingchun,MAO Weijian,WANG Yifu,et al.Experimental Study on the Effects of Panax Notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells and the MMPs/TIMPs Balance in a Mice with Abdominal Aortic Aneurysm Model[J].Journal of Modern Laboratory Medicine,2026,41(03):177-181.[doi:10.3969/j.issn.1671-7414.2026.03.032]
点击复制

三七总皂苷对小鼠腹主动脉瘤模型血管平滑肌细胞表型转换及MMPs/TIMPs平衡影响的实验研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年03期
页码:
177-181
栏目:
论著
出版日期:
2026-05-13

文章信息/Info

Title:
Experimental Study on the Effects of Panax Notoginseng Saponins on Phenotype Transformation of Vascular Smooth Muscle Cells and the MMPs/TIMPs Balance in a Mice with Abdominal Aortic Aneurysm Model
文章编号:
1671-7414（2026）03-177-05
作者:
侯青春1，毛伟鉴1，王益夫1，杨馥锟1，钟清莲2，王俊伟1
1.自贡市第四人民医院小儿外科与血管外科，四川自贡 643000;2.自贡市妇幼保健院超声医学科，四川自贡，643000
Author(s):
HOU Qingchun1，MAO Weijian1，WANG Yifu1，YANG Fukun1，ZHONG Qinglian2，WANG Junwei1
1.Department of Pediatric Surgery and Vascular Surgery, Zigong Fourth People’s Hospital, Sichuan Zigong 643000, China;2.De-partment of Ultrasound Medicine, Zigong Maternal and Child Health Hospital, Sichuan Zigong 643000, China
关键词:
腹主动脉瘤; 三七总皂苷; 血管平滑肌细胞; 基质金属蛋白酶; 基质金属蛋白酶组织抑制剂
分类号:
R543.16；R-332；R446
DOI:
10.3969/j.issn.1671-7414.2026.03.032
文献标志码:
A
摘要:
目的?探究三七总皂苷（PNS）对小鼠腹主动脉瘤（AAA）模型血管平滑肌细胞（VSMCs）表型转换及基质金属蛋白酶/基质金属蛋白酶组织抑制剂（MMPs/TIMPs）平衡的影响。方法小鼠随机分为对照组、模型组、PNS低剂量（PNS-Low）组、PNS高剂量（PNS-High）组。通过皮下植入渗透性微泵构建血管紧张素Ⅱ（AngⅡ）诱导的AAA小鼠模型。治疗结束后，观察腹主动脉形态并测量直径；检测血清肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β、IL-6水平；弹力纤维染色观察腹主动脉纤维断裂；检测VSMCs收缩型标志物[α-平滑肌肌动蛋白（α-SMA）、平滑肌蛋白22α（SM22α）]、合成型标志物[骨桥蛋白（OPN）]及MMPs/TIMPs相关蛋白（MMP1、MMP2、MMP9、TIMP1、TIMP2）表达。结果与对照组比较，模型组小鼠腹主动脉直径增大、弹力纤维大量断裂，血清TNF-α、IL-1β、IL-6水平升高，腹主动脉α-SMA、SM22α、TIMP1、TIMP2表达降低，OPN、MMP1、MMP2、MMP9表达升高，差异具有统计学意义（t=13.716～24.684，均P<0.05）；与模型组比较，PNS-Low组、PNS-High组小鼠腹主动脉直径减小，弹力纤维少量断裂，血清TNF-α、IL-1β、IL-6水平降低，腹主动脉α-SMA、SM22α、TIMP1、TIMP2表达升高，OPN、MMP1、MMP2、MMP9表达降低，差异具有统计学意义（t=7.509～23.952，均P<0.05）；与PNS-Low组比较，PNS-High组小鼠腹主动脉直径减小，弹力纤维少量断裂，血清TNF-α、IL-1β、IL-6水平降低，腹主动脉α-SMA、SM22α、TIMP1、TIMP2表达升高，OPN、MMP1、MMP2、MMP9表达降低，差异具有统计学意义（t=3.744～19.799，均P<0.05）。结论PNS能够抑制AAA小鼠VSMCs表型转换，并调节MMPs/TIMPs平衡。
Abstract:
Objective To investigate the effects of Panax notoginseng saponins (PNS) on the phenotype transformation of vascu-lar smooth muscle cells (VSMCs) and the matrix metalloproteinases/tissue inhibitors of matrix metalloproteinases (MMPs/TIMPs) balance in a mice abdominal aortic aneurysm (AAA) model. Methods Mice were randomly divided into a control group, a model group, a low-dose PNS (PNS-Low) group, and a high-dose PNS (PNS-High) group. Subcutaneous implantation of a per-meable micropumps was used to construct an angiotensin Ⅱ (Ang Ⅱ)-induced AAA mice model. After treatment, the morphol-ogy of the abdominal aorta was observed and its diameter was measured. Serum levels of TNF-α, interleukin (IL)-1β, and IL-6 levels were detected. Elastic Van Gieson staining was performed to observe fiber rupture in the abdominal aorta. The expression of contractile markers [α-smooth muscle actin (α-SMA), smooth muscle 22α (SM22α)], synthetic markers [osteopontin (OPN)], and MMPs/TIMPs related protein (MMP1, MMP2, MMP9, TIMP1, TIMP2) in VSMCs were detected. Results Com-pared with the control group, mouse in the model group exhibited increased abdominal aorta diameter, extensive rupture of elastic fibers, elevated serum levels of TNF-α, IL-1β, and IL-6., decreased expression of α-SMA, SM22α, TIMP1, and TIMP2 while increased expression of OPN, MMP1, MMP2, and MMP9 in abdominal aorta, with statistically significant differences (t=13.716～24.684, all P<0.05). Compared with the model group, mice in the PNS-Low and PNS-High groups exhibited reduced abdominal aorta diameter, minimal rupture of elastic fibers, decreased serum levels of TNF-α, IL-1β, and IL-6, increased ex-pression levels of α-SMA, SM22α, TIMP1 and TIMP2 , while decreased expression levels of OPN, MMP1, MMP2,and MMP9 in abdominal aorta, with statistically significant differences (t=7.509～23.952, all P<0.05). Compared with PNS-Low group, mice in the PNS-High group exhibited a reduced abdominal aorta diameter, minor rupture of elastic fibers, decreased se-rum levels of TNF-α, IL-1β, and IL-6, increased expression of α-SMA, SM22α, TIMP1, TIMP2 while decreased expression of OPN, MMP1, MMP2, and MMP9 in abdominal aorta, with statistically significant differences (t=3.744～19.799, all P<0.05). Conclusions Panax notoginseng saponins can inhibit phenotype transition of VSMCs and regulate the MMPs/TIMPs balance in mice with abdominal aortic aneurysm.

参考文献/References:

[1] ALI Z, KIM L, GUPTA K. Endovascular abdominal aortic aneurysm repair[J]. Interventional Cardiology Clinics,2025,14(2):173-190.
[2] YANG H Q, LI Z W, DONG X X, et al. Vinpocetine al-leviates the abdominal aortic aneurysm progression via VSMCs SIRT1-p21 signaling pathway[J]. Acta Phar-macologica Sinica,2025,46(1):96-106.
[3] ZHANG Q Y, CAI Z Y, YU Z W, et al. Agathis dam-mara extract and its monomer araucarone attenuate abdominal aortic aneurysm in mice[J]. Cardiovascular Drugs and Therapy,2025,39(2):239-257.
[4] NILSSON N, ALIM M D A, DIETRICH-ZAGO-NEL F, et al. The delayed presentation of achilles tendon ruptures is associated with marked alterations in the gene expression of COL1A1, MMPs, TIMPs, and IL-6[J]. American Journal of Sports Medi-cine,2024,52(1):164-173.
[5] DAVIS F M, MELVIN W J, MANGUM K, et al. The histone methyltransferase SETDB2 modulates tissue inhibitors of metalloproteinase-matrix metalloprotein-ase activity during abdominal aortic aneurysm develop-ment[J]. Annals of Surgery,2023,278(3):426-440.
[6] WANG L, SHAO L, GAO Y C, et al. Panax no-toginseng saponins alleviate inflammatory bowel disease via alteration of gut microbiota-bile acid metabolism[J]. American Journal of Chinese Medi-cine,2025,53(2):567-596.
[7] 朱熹萌. 三七总皂苷对大鼠血管平滑肌细胞增殖及cyclinD1?PCNA蛋白表达的影响[J]. 世界最新医学信息文摘,2016,16(90):132-133. ZHU X M. Effects of Panax notoginseng saponins on pro-liferation of vascular smooth muscle cells and expression of cyclinD1 and PCNA proteins in rats[J]. Word Latest Medicine Information,2016,16(90):132-133.
[8] LIU N, SHAN D Z, LI Y, et al. Panax notoginseng saponins attenuate phenotype switching of vascular smooth muscle cells induced by Notch3 silencing[J]. Evidence-Based Complementary and Alternative Medi-cine,2015,2015:162145.
[9] 周建国, 龙霖梓, 白瑞娜, 等. 三七总皂苷对载脂蛋白E基因敲除小鼠动脉粥样硬化斑块的影响[J]. 世界中西医结合杂志,2018,13(9):1240-1243. ZHOU J G, LONG L Z, BAI R N, et al. Impacts of Panax notoginseng saponins on atherosclerot-ic plague in ApoE knockout mice[J]. World Jour-nal of Integrated Traditional and Western Medi-cine,2018,13(9):1240-1243.
[10] 陈啸, 王晋军, 张林林, 等. LncRNA p21调控Hip-po-YAP信号通路对小鼠腹主动脉瘤形成的影响及机制[J]. 中国药理学通报,2024,40(1):55-62. CHEN X, WANG J J, ZHANG L L, et al. Effect of LncRNA p21 regulating Hippo-YAP signaling pathway on formation of abdominal aortic aneurysm in mice and its mechanism[J]. Chinese Pharmacological Bulle-tin,2024,40(1):55-62.
[11] 朱军凤, 姜旭, 陈溢滢, 等. 三七总皂苷对ApoE-/-小鼠动脉粥样硬化斑块形成及自噬的影响[J]. 河南中医,2023,43(3):378-384. ZHU J F, JIANG X, CHEN Y Y, et al. The influence of Panax notoginseng sponins on atherosclerotic plaque formation and autophagy in ApoE-/- mice[J]. Henan Traditional Chinese Medicine,2023,43(3):378-384.
[12] WANG Q H, LI N, GUO X, et al. Comprehensive anal-ysis identified a reduction in ATP1A2 mediated by ARI-D3A in abdominal aortic aneurysm[J]. Journal of Cellu-lar and Molecular Medicine,2022,26(10):2866-2880.
[13] WANG J J, YE W L, ZOU J M, et al. Targeting the smooth muscle cell Keap1-Nrf2-GSDMD-py-roptosis axis by cryptotanshinone prevents ab-dominal aortic aneurysm formation[J]. Theranos-tics,2024,14(17):6516-6542.
[14] YU Z P, WANG Y K, WANG X Y, et al. Smooth-mus-cle-cell-specific deletion of CD38 protects mice from AngII-induced abdominal aortic aneurysm through inhibiting vascular remodeling[J]. International Journal of Molecular Sciences,2024,25(8):4356.
[15] 赵培, 李永辉, 高伟, 等. 三七总皂苷通过调节TLR4/SYK信号抑制ApoE 基因敲除小鼠动脉粥样硬化泡沫细胞的形成[J]. 天然产物研究与开发,2021,33(8):1267-1273. ZHAO P, LI Y H, GAO W, et al. Panax notoginseng sa-ponins inhibited the formation of atherosclerotic foam cells in ApoE knockout mice by regulating TLR4 /SYK signaling[J]. Natural Product Research and Develop-ment,2021,33(8):1267-1273.
[16] 官兵, 张莹, 韦旭斌, 等. 三七总皂苷注射液对高脂饮食兔主动脉粥样硬化形成的干预作用[J]. 辽宁中医杂志,2019,46(8):1736-1739. GUAN B, ZHANG Y, WEI X B, et al. Effects of Pa-nax notoginseng saporins injection on atherosclerosis formation of aortic artery in experimental rabbits[J]. Liaoning Journal of Traditional Chinese Medi-cine,2019,46(8):1736-1739.
[17] SARACINI C, BOLLI P, STICCHI E, et al. Polymor-phisms of genes involved in extracellular matrix re-modeling and abdominal aortic aneurysm[J]. Journal of Vascular Surgery,2012,55(1):171-179, e2.
[18] PIECHOTA-POLANCZYK A, DEMYANETS S, MIT-TLBOECK M, et al. The influence of simvastatin on NGAL, matrix metalloproteinases and their tissue in-hibitors in human intraluminal thrombus and abdominal aortic aneurysm tissue[J]. European Journal of Vascular and Endovascular Surgery,2015,49(5):549-555.
[19] 孙道涵. O304通过激活AMPK/mTOR/MMPs通路缓解腹主动脉瘤形成[D]. 锦州:锦州医科大学,2024. SUN D H. O304 alleviates abdominal aortic aneurysm formation by activating AMPK/mTOR/MMPs path-way[D]. Jinzhou: Jinzhou Medical University,2024.

备注/Memo

备注/Memo:
作者简介：侯青春（1991-），男，硕士，主治医师，研究方向：血管外科，E-mail：1028180100@qq.com。
通讯作者：王俊伟（1976-），男，硕士，副主任医师，研究方向：血管外科，E-mail：silence_1976@163.com。
更新日期/Last Update: 2026-05-15