[1]董利平,马 丽.针刺通过CCL2/CCR2通路干预铁死亡对新生大鼠缺血缺氧性脑损伤模型修复机制的实验研究[J].现代检验医学杂志,2026,41(03):182-187.[doi:10.3969/j.issn.1671-7414.2026.03.033]
　DONG Liping,MA Li.Experimental Study on the Mechanism of Acupuncture-Mediated Repair of Hypoxic-Ischemic Brain Damage in Neonatal Rat Models through Intervening Ferroptosis via the CCL2/CCR2 Pathway[J].Journal of Modern Laboratory Medicine,2026,41(03):182-187.[doi:10.3969/j.issn.1671-7414.2026.03.033]
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针刺通过CCL2/CCR2通路干预铁死亡对新生大鼠缺血缺氧性脑损伤模型修复机制的实验研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年03期
页码:
182-187
栏目:
论著
出版日期:
2026-05-13

文章信息/Info

Title:
Experimental Study on the Mechanism of Acupuncture-Mediated Repair of Hypoxic-Ischemic Brain Damage in Neonatal Rat Models through Intervening Ferroptosis via the CCL2/CCR2 Pathway
文章编号:
1671-7414（2026）03-182-06
作者:
董利平1，马 丽2
1.武威市凉州医院风湿免疫科，甘肃武威 733000;2.武威市人民医院肾内科，甘肃武威 733000
Author(s):
DONG Liping1，MA Li2
1.Department of Rheumatology and Immunology, Liangzhou Hospital of Wuwei City, Gansu Wuwei 733000, China;2.Department of Nephrology, Wuwei People’s Hospital, Gansu Wuwei 733000, China
关键词:
针刺; 缺血缺氧性脑损伤; 趋化因子配体2/趋化因子配体2受体; 铁死亡; 炎症反应
分类号:
R722.1；R-332
DOI:
10.3969/j.issn.1671-7414.2026.03.033
文献标志码:
A
摘要:
目的探究针刺通过趋化因子配体2（CCL2）/CCL2受体（CCR2）通路对缺血缺氧性脑损伤（HIBD）新生大鼠修复的作用机制。方法将40只新生SD大鼠随机分为4组：假手术组、模型组、去铁胺组、针刺组，每组10只。除假手术组其余大鼠行左颈总动脉分离并结扎，3h后放入缺氧箱持续2h构建HIBD模型。去铁胺组腹腔注射0.2mg/g/天去铁胺，针刺组予针刺“水沟”“内关”“三阴交”，每日2次，均持续1周。神经学评分评估大鼠神经行为学功能；ELISA法检测血清肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、IL-1β水平；干湿重法检测脑组织含水量；HE染色观察海马组织病理学变化；比色法检测脑组织中总铁、脂质过氧化物（LPO）、丙二醛（MDA）、谷胱甘肽（GSH）含量；透射电镜观察海马组织神经元线粒体结构；Westernblot检测海马组织中谷胱甘肽过氧化物酶4（GPX4）、二价金属转运体1（DMT1）、膜铁转运蛋白1（FPN1）、转铁蛋白受体1（TFR1）、CCL2、JAK2、磷酸化JAK2（p-JAK2）、信号转导子和转录激活子3（STAT3）、磷酸化STAT3（p-STAT3）蛋白表达。结果与假手术组比较，模型组大鼠神经学评分、脑组织含水量、血清TNF-α、IL-6、IL-1β、海马组织中总铁、LPO、MDA含量及DMT1、TFR1、CCL2、p-JAK2/JAK2、p-STAT3/STAT3蛋白表达水平升高（t=5.56~37.02，均P<0.05），GSH含量及GPX4、FPN1蛋白表达水平降低（t=4.94~15.77，均P<0.05），组织病理损伤明显且线粒体呈铁死亡特征；与模型组比较，去铁胺组和针刺组大鼠神经学评分、脑组织含水量降低（t=2.33~5.33，均P<0.05），血清TNF-α、IL-6、IL-1β水平降低（t=8.02~30.61，均P<0.05），海马组织中总铁、LPO、MDA含量及DMT1、TFR1、CCL2、p-JAK2/JAK2、p-STAT3/STAT3蛋白表达水平降低（t=7.22~144.97，均P<0.05），GSH含量及GPX4、FPN1蛋白表达水平升高（t=2.70~16.12，均P<0.05），组织病理和线粒体铁死亡改变减轻。结论针刺可能通过下调CCL2/CCR2通路降低炎症反应，抑制海马组织铁死亡，从而修复新生大鼠缺血缺氧性脑损伤。
Abstract:
Objective To investigate the mechanism by which acupuncture exerts its effects hypoxic-ischemic brain damage (HIBD) in newborn rats via C-C Motif Ligand (CCL) 2/CCL receptor (CCR) 2 pathway. Methods Forty newborn Sprague-Daw-ley rats were randomly divided into four groups: a sham surgery group, a model group, a deferoxamine-treated group, and an acupuncture-treated group, with 10 rats in each group. Apart from the sham surgery cohort, the other rats underwent left common carotid artery dissection and ligation. Three hours later, they were placed in a hypoxic chamber for 2 hours to es-tablish the HIBD model. The deferoxamine group received daily intraperitoneal injections of 0.2 mg/g/day deferoxamine, whereas the acupuncture group underwent twice-daily acupuncture sessions at "Shuigou""Neiguan" and "Sanyinjiao" for a week. Neurobehavioral assessments were conducted using neurological scoring. ELISA assays were used to measure serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β. The dry-wet weight method was used to as-sess brain tissue water content. HE staining was used to inspect pathological changes in hippocampal tissue. The colorimetric assays were conducted to measure total iron, lipid peroxides (LPO), malondialdehyde (MDA), and glutathione (GSH) levels in brain tissue. Transmission electron microscopy was used to observe the mitochondrial structure of neurons in hippocampal tissue. Western blot analysis was performed to detect the protein expression of glutathione peroxidase 4 (GPX4), divalent metal transporter 1 (DMT1), Ferroportin 1 (FPN1), transferrin receptor 1 (TFR1), CCL2, JAK2, phosphorylated JAK2 (p-JAK2), signal transducer and activator of transcription 3 (STAT3), and phosphorylated STAT3 (p-STAT3) in hippocampal tissue. Results Com-pared with the sham operation group, rats in the model group exhibited higher neurological scores, brain tissue water content (t=12.65, 5.56, both P<0.05), serum TNF-α, IL-6, and IL-1β levels (t=28.10~49.24, all P<0.05), total iron, LPO and MDA, as well as protein levels of DMT1, TFR1, CCL2, p-JAK2/JAK2 and p-STAT3/STAT3 (t=7.42~37.02, all P<0.05). However, the content of GSH and the protein expression levels of GPX4 and FPN1 were decreased (t=4.94~15.77, all P<0.05). There was ob-vious histopathological damage, and the mitochondria showed characteristics of ferroptosis. Compared with the model group, the deferoxamine group and the acupuncture group showed reduced neurological scores and brain tissue water content (t=2.33~5.33, all P<0.05), serum levels of TNF-α, IL-6 and IL-1β(t=8.02~30.61, all P<0.05), the levels of total iron, LPO and MDA in hip-pocampal tissue, and the protein expression levels of DMT1, TFR1, CCL2, p-JAK2/JAK2 and p-STAT3/STAT3 (t=7.22~144.97, all P<0.05), while the content of GSH and the protein expression levels of GPX4 and FPN1 were increased (t=2.70~16.12, all P<0.05). The histopathological and mitochondrial changes associated with ferroptosis were alleviated. Conclusions Acupuncture may reduce inflammatory responses by downregulating the CCL2/CCR2 pathway, thereby inhibiting ferroptosis in hippocampal tissue and facilitating the repair of hypoxic-ischemic brain injury in newborn rats.

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备注/Memo

备注/Memo:
基金项目：甘肃省卫生健康委员会科研项目（20231479），武威市市级科技计划项目（WW23B02SF069）。
作者简介：董利平（1986-），男，本科，主治医师，研究方向：内科疾病的针灸防治，E-mail：vmrytmx15@163.com。
更新日期/Last Update: 2026-05-15