[1]徐 岩,阿依吐兰·买买提,丁剑冰.慢性阻塞性肺疾病患者血清胞浆型磷脂酶A2α基因和表达水平检测的临床意义[J].现代检验医学杂志,2015,30(02):97-99.[doi:10.3969/j.issn.1671-7414.2015.02.030]
　XU Yan,AYITULAN·Maimaiti,DING Jian-bing.Clinical Significant of Detecting the Cytoplasm of Phospholipase A2α Expression in Patints with Chronic Obstructive Pulmonary Disease[J].Journal of Modern Laboratory Medicine,2015,30(02):97-99.[doi:10.3969/j.issn.1671-7414.2015.02.030]
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慢性阻塞性肺疾病患者血清胞浆型磷脂酶A2α基因和表达水平检测的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
97-99
栏目:
研究简报·实验技术
出版日期:
2015-03-20

文章信息/Info

Title:
Clinical Significant of Detecting the Cytoplasm of Phospholipase A2α Expression in Patints with Chronic Obstructive Pulmonary Disease
作者:
徐 岩1; 阿依吐兰·买买提2; 丁剑冰1
1.新疆医科大学基础医学院, 乌鲁木齐 830011; 2.新疆和田地区于田县医院检验科,新疆和田 848400
Author(s):
XU Yan1; AYITULAN·Maimaiti2; DING Jian-bing1
1.Primary Medicine Courtyard Affiliated Xinjiang University,Urumqi 830011, China; 2.Laboratory Department of Yutian Hospital,Xinjiang Hetian 848400,China
关键词:
胞浆型磷脂酶A2α;  逆转录聚合酶链式反应;  慢性阻塞性肺疾病
分类号:
R563; Q786
DOI:
10.3969/j.issn.1671-7414.2015.02.030
文献标志码:
A
摘要:
目的 探讨慢性阻塞性肺疾病(COPD)患者检测胞浆型磷脂酶cPLA2α(cPLA2α)基因表达水平的临床意义。方法 选取收治的COPD患者90例,按照COPD严重程度分级标准分为轻度组30例,中度组35例和重度组25例,以同期90例肺功能正常的健康体检者为对照组。用酶联免疫吸附试验(ELISA)检测受试者血清中cPLA2α含量,同时用逆转录聚合酶链式反应(RT-PCR)检测同组患者cPLA2α基因表达水平。结果 COPD轻度组cPLA2α的含量为(0.039 2±0.005 1)pg/ml,表达为(0.68±0.01); 中度组含量为(0.049 8±0.007 4)pg/ml,表达为(0.92±0.02); 重度组含量为(0.055 4±0.008 1)pg/ml,表达为(1.35±0.02); 而对照组含量为(0.010 2±0.006 6)pg/ml,表达为(0.11±0.01)。无论是cPLA2α血清学水平还是基因表达水平,四组之间差异均有统计学意义(F=8.643,12.415,P<0.05)。且COPD各组随着病变程度加重cPLA2α的水平逐渐增加,且三组彼此比较差异有统计学意义(t=3.013～5.817,5.039～11.667,P均<0.05)。结论 血cPLA2α水平可预示COPD严重程度,cPLA2α是诊疗COPD及分级的新靶标。
Abstract:
Objective To discuss the clinical significant of detecting cytoplasm phospholipase A2 alpha(cPLA2α)in patients with chronicobstructive pulmonary disease(COPD).Methods Selected treated 90 cases of patients with COPD.According to the COPD severity classification standard,they were divided into mild group of 30 cases,moderate group of 25 cases and severe group of 35 cases.In the same period of physical examination,90 cases of normal lung function healthy were control group.Used Enzyme linked immunosorbent test(ELISA)to detect cPLA2α level of these groups.At the same time,detected cPLA2α expression of these groups by RT-PCR.Results cPLA2α level in serum of mild group was(0.039 2±0.005 1)pg/ml,gene expression was(0.68±0.01),in moderate group(0.049 8±0.007 4)pg/ml and(0.92±0.02),in severe group(0.055 4±0.008 1)and(1.35±0.02).and in Healthy controls group(0.010 2±0.006 6)pg/ml and(0.11±0.01).There were significant differences among four groups(t=3.013～5.817,5.039～11.667,P<0.05).Conclusion Detection of blood cPLA2α can indicate COPD severity,and cPLA2α is the new molecular targets of diagnosis,treatment and classification COPD.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:徐 岩(1987-),女,硕士研究生在读,研究方向:感染免疫。 通讯作者:丁剑冰,博士,教授,E-mail:djbing002@163.com。
更新日期/Last Update: 2015-03-20