[1]安 哲,李思鹏,董 炜,等.ELISA法检测HBV-M结果呈抗HBc(-)抗HBe(+)的科学评价[J].现代检验医学杂志,2015,30(04):99-101.[doi:10.3969/j.issn.1671-7414.2015.04.028]
　AN Zhe,LI Si-peng,DONG Wei,et al.Reality of Negative anti-HBc and Positive anti-HBe Results of Testing HBV-M with ELISA[J].Journal of Modern Laboratory Medicine,2015,30(04):99-101.[doi:10.3969/j.issn.1671-7414.2015.04.028]
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ELISA法检测HBV-M结果呈抗HBc(-)抗HBe(+)的科学评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
99-101
栏目:
研究简报·实验技术
出版日期:
2015-08-10

文章信息/Info

Title:
Reality of Negative anti-HBc and Positive anti-HBe Results of Testing HBV-M with ELISA
文章编号:
1671-7414(2015)04-099-03
作者:
安 哲; 李思鹏; 董 炜; 张 妮; 李妙羡; 王香玲
西安交通大学医学院第二附属医院检验科,西安 710004
Author(s):
AN Zhe; LI Si-peng; DONG Wei; ZHANG Ni; LI Miao-xian; WANG Xiang-ling
Department of Clinical Laboratory,the Second Affiliated Hospital of Medical College,Xi'an Jiaotong University,Xi'an 710004,China
关键词:
抗HBe;  抗HBc;  酶联免疫吸附法;  化学发光微粒子免疫测定
分类号:
R512.62; R446.61
DOI:
10.3969/j.issn.1671-7414.2015.04.028
文献标志码:
A
摘要:
目的 对ELISA法检测HBV-M呈抗HBc阴性、抗HBe阳性结果的科学评价。方法 ELISA法初检HBV-M呈抗HBc阴性,抗HBe阳性样本105例,以CMIA法为参考方法复检确证抗HBe和抗HBc并评价其真阳性率和假阴性率; 以其S/CO水平不同分组评价不同水平抗HBe和抗HBc检测结果的真实性。结果 CMIA法复检后产生3种不同结果,即抗HBc阳性/抗HBe阳性、抗HBc阴性/抗HBe阴性、抗HBc阳性/抗HBe阴性,分别占72.38%,21.91%和5.71%,抗HBc假阴性率和抗HBe真阳性率分别为78.10%和72.38%; 在0.00～0.10,0.10～0.80和0.80～1.00不同S/CO水平内抗HBe真阳性率分别为42.86%,88.14%和56.25%,在1.00～1.20,1.20～2.00和2.00～3.00不同S/CO水平内抗HBc假阴性率分别为93.33%,96.15%和47.37%。结论 ELISA法检测HBV-M呈抗-HBc阴性/抗HBe阳性结果对HBV既往感染有重要的提示价值,但不能反映真实结果,存在三种可能,应进一步复检确证。
Abstract:
Objective To research the reality of ELISA testing results with negative anti-HBc and positive antiHBe.Methods CMIA was carried outto retest antiHBc and antiHBe of 105 samples which were initially tested to have negative antiHBc and positive antiHBe.Results Among the 105 samples retested by CMIA,there were three different results,positive antiHBc with positive antiHBe,negative antiHBc with negative antiHBe and positiveantiHBc with negative antiHBe,whose proportions were 72.38%,21.91% and 5.71% respectively; the fasle negative rates of antiHBc were 78.10% in total and 93.33%,96.15% and 47.37% in 3 subgroup with S/CO 1.00～1.20,1.20～2.00 and 2.00～3.00,respectively; the true positive rates of antiHBe were 72.38% intotal and 42.86%,88.14% and 56.25% in 3 subgroups with S/CO 0.00～0.10,0.10～0.80 and 0.80～1.00,respectively.Conclusion HBV-M results with negative antiHBc and positive antiHBe by ELISA could give suggestive value and not reflect true information about antiHBc and antiHBe with three alternatives which would be obtained through retesting by a second assay.

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备注/Memo

备注/Memo:
作者简介:安 哲(1980-),男,硕士,主管检验师,研究方向为病毒性肝炎的实验室检测和诊断,E-mail:anzhe80@126.com。
更新日期/Last Update: 2015-08-10