[1]刘文静,谢秀丽,宋红梅,等.PREVI Isola自动化微生物接种仪性能评估[J].现代检验医学杂志,2016,31(01):108-111.[doi:10.3969/j.issn.1671-7414.2016.01.031]
　LIU Wen-jing,XIE Xiu-li,SONG Hong-mei,et al.Evaluation of PREVI Isola Automated Plate Streaker[J].Journal of Modern Laboratory Medicine,2016,31(01):108-111.[doi:10.3969/j.issn.1671-7414.2016.01.031]
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PREVI Isola自动化微生物接种仪性能评估()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
108-111
栏目:
研究简报·实验技术
出版日期:
2016-01-25

文章信息/Info

Title:
Evaluation of PREVI Isola Automated Plate Streaker
作者:
刘文静; 谢秀丽; 宋红梅; 刘世豪; 杨启文; 徐英春
北京协和医院检验科,北京 100730
Author(s):
LIU Wen-jing; XIE Xiu-li; SONG Hong-mei; LIU Shi-hao; YANG Qi-wen; XU Ying-chun
Department of Clinical Laboratory, Peking Union Medical CollegeHospital,Beijing 100730,China
关键词:
PREVI Isola;  微生物前处理;  自动接种仪
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2016.01.031
文献标志码:
A
摘要:
目的 评估PREVI Isola自动化微生物接种仪的性能。方法 收集北京协和医院送检呼吸道标本80份、无菌体液70份、粪便52份、拭子69份、脑脊液12份、尿液80份。同一份标本分别按照常规手工和PREVI Isola接种仪标本操作流程进行接种,通过与手工法相比,对PREVI Isola进行评估。结果用SPSS16.0进行统计分析。结果 PREVI Isola自动化程度高,可选择合适的接种平板和贴上标签记录接种平板的类型和接种时间等; PREVI Isola可精确地吸取标本并使用独特的接种涂布器分离接种,相当于17个接种环并排使用,划线模式重复性好; 标本分离有效性:分离得到的菌种数PREVI Isola仪器法与手工法呼吸道标本和粪便标本具有统计学差异,手工法分离得到的菌种数多于PREVI Isola仪器法,其它标本类型两种方法无统计学差异; 均分离菌株单个菌落个数,PREVI Isola仪器法分离得到菌株单个菌落的个数均多于手工法,呼吸道标本、无菌体液和粪便标本有统计学差别,其它3种标本拭子、脑脊液和尿液标本无统计学差异; 均能分离得到菌株的半定量结果,呼吸道标本和尿液标本有统计学差异,PREVI Isola仪器法分离得到菌株的分布大于手工法。其它标本无统计学差异。接种效率:如果同批次接种的标本类型比较单一(主要为尿液等液体标本),且接种的平板类型相同,则仪器的接种效率比手工法高。如果同批次接种的标本类型比较复杂,相比手工法仪器的接种效率低; 另外,并不是所有的标本都适合于用自动化接种仪进行接种,例如:脑脊液、导管、组织等。结论 如果实验室的标本类型比较单一,或优化所需接种琼脂平板类型一致的不同标本同时进行接种,PREVI Isola是一款高效的自动化接种仪。
Abstract:
Objective To evaluate the characteristics of the PREVI Isola automated plate streaker(bioMérieux,SA).Methods 80 respiratory tract specimens,70 sterile fluids,52 stools,69 swabs,12 cerebrospinal fluids and 80 urines were collected in Peking Union Medicd College Hospital.Specimens were processed with manual streaking and PREVI Isola system.PREVI Isola system were evaluated comparing to the manual streaking.Thequality of results were analyzed by SPSS 16.0,doing Wilcoxon's Sign Rank Testfor the results of finally isolated species,overall numbers of isolated colonies and semi-quantitative of the species were both isolated by the two methods.Results PREVI Isola system was highly automatic,which could select the right plates and stake the bar code on the back of the plate indicating the type of the agar and inoculation time,PREVI Isola system could accurately absorb the liquid specimen and use a novel comb streaking procedure for processing of fluid specimens on standard agar plates,like 17 inoculating loops work together.It also had a good reproducibility.The quality of PREVI Isola system results:As to the finally isolated species,there was significant statistical difference between PREVI Isola system and manual streaking method in respiratory tract and stools specimen,there were more species isolated by manual streaking method than PREVI Isola system.There were no differences between the two methods for the other of specimen types.As to the amount of pure clones of the species were both isolated by the two methods,there were significant statisticaldifferences between the two methods for respiratory tract,sterile fluid and stool specimens.The amount of clones isolated by PREVI Isola system was more thanmanual streaking method.In semi-quantitative results,there were significant statistical differences between the two methods for respiratory tract and urine specimen,Species had wider distribution of PREVI Isola system than manual streaking method.Inoculation efficiency:if the batch of specimen type was simple(mainly the urine and so on),using the same plates,PREVI Isola system was more efficient than manual streaking method.However,if the batch of specimen type wascomplicated,manual method was high-performance.Besides,not all specimen type could be inoculated by PREVI Isola system,such as cerebrospinal fluid,catheter and tissues.Conclusion If the lab had simple specimentype,or utilize the specimen type using the same agar plates to be inoculatedtogether,PREVI Isola system belongs to a good performance automated plate streaker.

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备注/Memo

备注/Memo:
作者简介:刘文静(1984-),女,硕士,实习研究员,主要从事临床微生物及分子检验,Tel:13401082194,E-mail:liuwenjing1220@139.com。 通讯作者:徐英春(1964-),男,本科,研究员,博士生导师,研究方向:临床微生物,Tel:010-69159766,E-mail:xycpumch@139.com。
更新日期/Last Update: 2016-01-20