[1]陈妍珂,王孝功,王亚鹏.改良的Tricine-SDS-PAGE电泳检测胸腺肽分子量[J].现代检验医学杂志,2016,31(01):132-133,137.[doi:10.3969/j.issn.1671-7414.2016.01.038]
　CHEN Yan-ke,WANG Xiao-gong,WANG Ya-peng.Analysis of the Molecular Weight of Peptides in Thymosin by Tricine-SDS-PAGE[J].Journal of Modern Laboratory Medicine,2016,31(01):132-133,137.[doi:10.3969/j.issn.1671-7414.2016.01.038]
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改良的Tricine-SDS-PAGE电泳检测胸腺肽分子量()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
132-133,137
栏目:
研究简报·实验技术
出版日期:
2016-01-25

文章信息/Info

Title:
Analysis of the Molecular Weight of Peptides in Thymosin by Tricine-SDS-PAGE
作者:
陈妍珂1; 王孝功2; 王亚鹏2
1.西安交通大学医学部生物医学实验研究中心,西安 710061; 2.西安迪赛生物药业有限责任公司,西安 710016
Author(s):
CHEN Yan-ke1; WANG Xiao-gong2; WANG Ya-peng2
1.Experiment Center of Biomedical Research,Xi'an Jiaotong University School of Medicine, Xi'an 710061,China; 2.Xi'an Disai Biology Pharmaceutical Company,Xi'an 710016,China
关键词:
胸腺肽;  分子量;  Tricine-SDS-PAGE电泳;  胸腺肽α1
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2016.01.038
文献标志码:
A
摘要:
目的 建立一种分析胸腺肽制剂中多肽分子量分布的简便可行的方法。方法 采用改良的Tricine-SDS-PAGE电泳方法分析胸腺肽制剂中多肽的分子量分布,并分析其中胸腺素α1的含量。结果 采用该法可检出1μg的胸腺肽α1,分析国内市场上的胸腺肽制剂多肽分子量分布范围在3.5～8.5kD。结论 该法适用于分析胸腺肽制剂中多肽组分的分子量分布和胸腺肽α1的存在,可用于胸腺肽制剂的质量控制。
Abstract:
Objective To establish a simple and feasible method in analyzing the molecular weight distribution of peptides in thymosin.Methods Improved Tricine-SDS-PAGE electrophoresis was applied to detect the molecular weight of peptides and the content of thymosin α1 in thymosin.Results Tested the thymosin preparations on the domestic market using this improved method.It was demonstrated that the peptide molecular weight distribution in thymosin preparations was between 3.5～8.5 kD,also could detect the concentration of 1 μg thymosin α1 in thymosin by using this improved method.Conclusion This improved method is suitable for the analysis of peptides molecular weight distribution and theconcentration of thymosin α1,so it can be used for control quality of thymosin preparations.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家科技支撑计划“药品注射剂检测技术体系研究”(编号2008BAI55B04)。 作者简介:陈妍珂(1973-),女,蒙古族,博士,讲师,研究方向为肿瘤的发生发展机制,E-mail:yankechen@126.com。
更新日期/Last Update: 2016-01-20