[1]武冬娜,石喜习,聂 菲,等.HbF变异体对三种不同HbA1c检测系统的干扰评价[J].现代检验医学杂志,2016,31(02):99-102.[doi:10.3969/j.issn.1671-7414.2016.02.029]
　WU Dong-na,SHI Xi-xi,NIE Fei,et al.Evaluation of Interference of Variant Hemoglobin F on Three Different HbA1c Measurement Systems[J].Journal of Modern Laboratory Medicine,2016,31(02):99-102.[doi:10.3969/j.issn.1671-7414.2016.02.029]
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HbF变异体对三种不同HbA1c检测系统的干扰评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
99-102
栏目:
研究简报·实验技术
出版日期:
2016-06-01

文章信息/Info

Title:
Evaluation of Interference of Variant Hemoglobin F on Three Different HbA1c Measurement Systems
作者:
武冬娜; 石喜习; 聂 菲; 王晓倩; 贾 卉
西北妇女儿童医院检验科,西安 710061
Author(s):
WU Dong-na; SHI Xi-xi; NIE Fei; WANG Xiao-qian; JIAHui
Department of Clinical Laboratory, Northwest Women's and Children's Hospital,Xi'an 710061,China
关键词:
血红蛋白F;  血红蛋白A;  糖基化
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2016.02.029
文献标志码:
A
摘要:
目的 评价血红蛋白变异体F(HbF)对三种糖化血红蛋白(HbA1c)检测系统测定结果的干扰。三种方法分别为离子交换高效液相色谱法(IE-HPLC)、硼酸盐亲和层析高效液相色谱法(AC-HPLC)和免疫抑制比浊法。方法 分别用三种检测系统检测血红蛋白结构正常标本及含有胎儿血红蛋白(HbF)的标本,依据美国国家糖化血红蛋白标准化计划(NGSP)的判定标准,对检测结果进行比对分析和偏倚评估。结果 以Primus Ultra2(AC-HPLC)为比较系统,IE-HPLC和免疫比浊法为检测系统,两种检测系统与比较系统检测正常样品HbA1c值的相关性良好。当HbF≤8.75%时,IE-HPLC测定结果与比较系统偏差<6%; HbF为17.50%时,IE-HPLC测定结果与比较系统偏差>6%; 当HbF浓度达35.00%～70.00%时IE-HPLC无法检测出结果。免疫比浊法测定不同HbF浓度HbA1c值与比较系统偏差均<6%,测试几乎不受HbF的干扰。结论 HbF对不同HbA1c检测系统的干扰程度不同,临床实验室在进行HbA1c检测时,应注意血红蛋白变异体的存在,必要时选用替代指标或者合适的方法进行HbA1c测定以防止干扰的发生。
Abstract:
Objective To evaluate the interference of variant hemoglobin F(HbF)on three kinds of glycosylated hemoglobin(HbA1c)testingsystems.The three methods were ion exchange high performance liquid chromatography(HPLC)method,affinity hromatography HPLC method and mmunosuppression turbidimetry respectively.Methods To measure normal hemoglobin structure specimens and contain fetal hemoglobin(HbF)specimens with three measurement systems respectively,according to the standards of the National Glycated hemoglobin Standardization Program(NGSP),the results were comparatively analyzed and bias evaluated.Results Took the Primus Ultra2(AC-HPLC)as comparing system,the Variant II and immune turbid metric methodas the detection system.The testing results of those testing systems had good correlation for various concentrations with normal hemoglobin HbA1c blood samples.When the HbF ≤8.75%,the deviation was less than 6% between IE-HPLC determination and comparison system.When HbF was 17.50%,the deviation was greater than 6% between IE-HPLC determination and comparison system.When HbF concentration was 35.00%～70.00%,IE-HPLC could not be detected.The deviation was lessthan 6% between Immune turbid metric method and comparison system for different HbF concentration of HbA1c.The test was almost without any interference of HbF.Conclusion The interference degree of HbF on differentHbA1c detection systems were different when tested HbA1c in clinical laboratory,should pay attention to the existence of hemoglobin variant.If necessary,choose alternative indicators or appropriate method to determinate HbA1c to preventinterference.

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备注/Memo

备注/Memo:
作者简介:武冬娜(1976-),女,硕士,主管检验师,专业:生化检验,E-mail:dongna2860@sina.com。
更新日期/Last Update: 2016-02-20