[1]刘志远,潘 健,齐 杰,等.黄芩苷对一株产NDM-1 大肠埃希菌体内外抗菌作用的研究[J].现代检验医学杂志,2016,31(03):124-126.[doi:10.3969/j.issn.1671-7414.2016.03.035]
 LIU Zhi-yuan,PAN Jian,QI Jie,et al.Anti-bacterial Effects of Baicalin on One Escherichia coli Strain Carrying NDM-1 Gene[J].Journal of Modern Laboratory Medicine,2016,31(03):124-126.[doi:10.3969/j.issn.1671-7414.2016.03.035]
点击复制

黄芩苷对一株产NDM-1 大肠埃希菌体内外抗菌作用的研究()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
124-126
栏目:
研究简报·实验技术
出版日期:
2016-06-15

文章信息/Info

Title:
Anti-bacterial Effects of Baicalin on One Escherichia coli Strain Carrying NDM-1 Gene
作者:
刘志远潘 健齐 杰张婷菊马玉芝
中国中医科学院广安门医院检验科,北京 100053
Author(s):
LIU Zhi-yuanPAN JianQI JieZHANG Ting-juMAYu-zhi
Department of Clinical Laboratory,Guanganmen Hospital, China Academy of Chinese Medical Science,Beijing 100053,China
关键词:
黄芩苷 大肠埃希菌 NDM-1金属酶 抗菌作用
分类号:
R378.21; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.03.035
文献标志码:
A
摘要:
目的 观察黄芩苷对产NDM-1大肠埃希菌的体内外抗菌作用。方法 肉汤稀释法测定黄芩苷的最小抑菌浓度(MIC)、最小杀菌浓度(MBC); 棋盘联合药敏试验检测黄芩苷与亚胺培南的协同作用; 通过小鼠菌血症模型,观察黄芩苷对感染模型的保护作用。结果 黄芩苷的最小抑菌浓度和最小杀菌浓度相同,均为8 mg/ml; 黄芩苷与亚胺培南呈明显的协同作用(FIC=0.125),单独使用黄芩苷使菌血症小鼠死亡率降低25%。结论 黄芩苷体内、外均具有一定的抗产NDM-1大肠埃希菌活性。
Abstract:
Objective To examine the bacteriostasis of baicalin against Escherichia coli strain carrying NDM-1 gene.Methods Minimal inhibitory concentration(MIC)and minimal bactericidal concentration(MBC)were determined by broth dilution method.Synergy function was designed withcheckerboard method.Anti-infection effect in vivo of baicalin wasobserved in mice with bacteremia.Results In vitro antibacterial tests showed that the MIC and MBC baicalin were 8 mg/ml.Synergistic inhibitory effect was observed between baicalin and imipenem(FIC=0.125).In vivo inhibition experiments showed that baicalin had decreased the mortality of 25% of Escherichia coli Strain carrying NDM-1 gene infection in mice.Conclusion Baicalin has antibacterial effect on NDM-1 E.coli.

参考文献/References:

[1] Kumarasamy KK,Toleman MA,Walsh TR,et al.Emergence of a new antibiotic resistance mechanism in India,Pakistan,and the UK:a molecular,biological,and epidemiological study[J].Lancet Infect Dis,2010,10(10):597-602.
[2] Lai MY,Hsiu SL,Tsai SY,et al.Comparison of metabolic pharmacokineticsof baicalin and baicalein in rats[J].J Pharm Pharmacol,2003,55(2):205-209.
[3] 施高翔,邵 菁,汪天明,等.黄芩及其有效成分抗菌作用新进展[J].中国中药杂志,2014,39(19):3713-3718. Shi GX,Shao J,Wang TM,et al.New advance in studies on antimicrobal activity of scutellaria baicalensis and its effective ingredients[J].ChinaJournal of Chinese Meteria Medica,2014,39(19):3713-3718.
[4] Clinical and Laboratory Standards Institute.Performalice standards for antimicrobial susceptibility testing:Twenty First informational supplement[S].Wayne:Pa,CLSI M100-S11.
[5] 邹明祥,邬靖敏,李 军,等.产NDM-1肺炎克雷伯菌中国分离株的初步研究[J].中国当代儿科杂志,2012,14(8):616-621. Zou MX,Wu JM,Li J,et al.NDM-1 producing Klebsiella pneumoniae in mainland China[J].Chin J Contemp Pediatr,2012,14(8):616-621.
[6] 宁明哲,张之烽,周万青,等.耐亚胺培南的肺炎克雷伯菌碳青霉烯酶基因检测及其同源性分析[J].现代检验医学杂志,2014,29(2):58-59,63. Ning MZ,Zhang ZF,Zhou WQ,et al.Carbapenemase gene detection andhomology analysis of imipenem-resistant Klebsiella pneumoniae[J].J Mod Lab Med,2014,29(2):58-59,63.

相似文献/References:

[1]陈 菲,曹小利,程 莉,等.尿培养产CTX-M大肠埃希菌的种系分型及耐药和毒力特点分析[J].现代检验医学杂志,2016,31(02):12.[doi:10.3969/j.issn.1671-7414.2016.02.004]
 CHEN Fei,CAO Xiao-li,CHENG Li,et al.Analysis on the Resistance and Virulence Characteristics and Phylogenetic Groups of CTX-M-Producing Escherichia Coli Isolated from Urine[J].Journal of Modern Laboratory Medicine,2016,31(03):12.[doi:10.3969/j.issn.1671-7414.2016.02.004]
[2]靳占奎a,王 曦,杨 乐,等.重组人肿瘤坏死因子hTNF-α在大肠埃希菌[E.coli BL21DE3]中表达条件的优化[J].现代检验医学杂志,2017,32(05):100.[doi:10.3969/j.issn.1671-7414.2017.05.017]
 JIN Zhan-kuia,WANG Xi,YANG Le,et al.Optimization of Expression of Recombinant Human Tumor Necrosis Factor(hTNF-α)in Escherichia coli[E.coli BL21(DE3)][J].Journal of Modern Laboratory Medicine,2017,32(03):100.[doi:10.3969/j.issn.1671-7414.2017.05.017]
[3]杨宏斌,张 妍.甘肃省天水地区产ESBLs大肠埃希菌致尿路感染的基因分型及耐药性分析[J].现代检验医学杂志,2019,34(03):55.[doi:10.3969/j.issn.1671-7414.2019.03.013]
 YANG Hong-bin,ZHANG Yan.Genotyping and Drug Resistance Analysis of UrinaryTract Infection Caused by ESBLs-producing Escherichia coliin Tianshui Area of Gansu Province[J].Journal of Modern Laboratory Medicine,2019,34(03):55.[doi:10.3969/j.issn.1671-7414.2019.03.013]

备注/Memo

备注/Memo:
基金项目:国家中医临床研究示范基地科研专项暨所级科研基金课题(2011S242)。作者简介:刘志远(1978-),男,硕士,主要从事细菌耐药机制及中药抗感染的研究,Tel:(010)88001460,E-mail:593793204@qq.com。
更新日期/Last Update: 2016-06-25