[1]刘 青,樊 冰.儿童呼吸道感染患者金黄色葡萄球菌的分离与耐药性分析[J].现代检验医学杂志,2016,31(03):150-152.[doi:10.3969/j.issn.1671-7414.2016.03.042]
　LIU Qing,FAN Bing.Separation and Analysis of Drug Resistance of Staphylococcus Aureus in Patients with Respiratory Infection of Children[J].Journal of Modern Laboratory Medicine,2016,31(03):150-152.[doi:10.3969/j.issn.1671-7414.2016.03.042]
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儿童呼吸道感染患者金黄色葡萄球菌的分离与耐药性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
150-152
栏目:
检验与临床
出版日期:
2016-06-15

文章信息/Info

Title:
Separation and Analysis of Drug Resistance of Staphylococcus Aureus in Patients with Respiratory Infection of Children
作者:
刘 青1; 樊 冰2
1.深圳市光明新区人民医院检验科,广东深圳 518106;
2.深圳市第二人民医院检验科,广东深圳 518000
Author(s):
LIU Qing1; FAN Bing2
1.Department of Clinical Laboratory,the People's Hospital of Guangming New Dictrict,Guangdong Shenzhen 518106,China;
2.Department of Clinical Laboratory,the Second People's Hospital of Shenzhen,Guangdong Shenzhen518000,China
关键词:
金黄色葡萄球菌;  耐甲氧西林金黄色葡萄球菌;  儿科;  呼吸道感染;  耐药性
分类号:
R378.11; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.03.042
文献标志码:
A
摘要:
目的 探讨和分析金黄色葡萄球菌在儿科呼吸道感染患者中的分离与耐药性变化,为指导临床用药控制感染提供依据。方法 采集2013年1月～2014年12月两年儿科(包括新生儿科)呼吸道感染患者痰液标本共5 413例,应用MicroScan-As4微生物自动鉴定分析仪对分离后的菌株进行鉴定和药物敏感试验,用Whonet5.6软件进行统计分析。结果 从儿科呼吸道感染患者标本中共分离出各种病原菌29种1 540株。其中金黄色葡萄球菌(S.aureus,SAU)336株(21.82%,336/1 540),超过总病原菌的1/5,在儿科呼吸道感染分离率占6.21%,显著高于其它病原菌分离率(除流感嗜血杆菌5%外,其余均<5%)。在所有检出SAU中耐甲氧西林金黄色葡萄球菌(MRSA)59株(17.56%,59/336),可以看出SAU在儿科呼吸道感染患者中占据首要位置。SAU对万古霉素、利奈唑胺、达托霉素、奎奴普丁这4种抗菌药物耐药率为0,对其它抗菌药物呈现不同程度耐药性; MRSA对青霉素、氨苄西林、苯唑西林、氨苄西林/舒巴坦、阿莫西林/克拉维酸、头孢唑林、头孢曲松耐药率为100%,对红霉素、克林霉素耐药率超过50%,对其它抗菌药物耐药性呈低耐药性。结论 儿童呼吸道感染SAU较多,尤其MRSA多重耐药现象严重。应加强对金黄色葡萄球菌耐药性监测,根据药敏试验结果进行个体化、合理化选用抗菌药物,有效控制和减少耐药菌株产生。
Abstract:
Objective Discuss and analyze Staphylococcu saureus in isolation and drug resistance in patients with pediatric respiratoryinfection,providing guidelines for clinical treatment on the control of infection.Approaches collect 5 413 cases of respiratory tract infection from Jan.2013 to Dec.2014 in patients(including pediatrics neonatology)with sputum specimens,applying MicroScan-As4 automated Microbes Identification analyzer to identify and test drug sensitiveness of the separated strains,using Whonet 5.6 forstatistical analysis.Results 1 540 strains of 29 different pathogenic bacteria were isolate from pediatric patients with respiratory tract infection,among which there were 336 strains(21.82%,336/1540)of Staphylococcus aureus(SAU,S.aureus),more than 1/5 of the total of pathogenic bacteria.And the isolation rate of pediatric respiratory tract infection accounted for 6.21%,significantly higher than that of other pathogenic bacteria isolation rate(with the exception of Haemophilus influenzae>5%,the rest all<5%).In all detected SAU,methicillin-resistant Staphylococcus aureus(MRSA)occpied 59 strains(17.56%,59/336),which indicated that SAU played a leading place in patients with pediatric respiratory infection.The antimicrobial drug resistance rate of SAU against vancomycin,Nai thiazoleamine,daptomycin,and Quinupristin was 0,whereas to other antibiotics it showed different degrees of resistance.The drug resistance rate of MRSA to penicillin and ampicillin,oxacillin,ampicillin/sulbactam,amoxicillin/clavulanic acid and cefazolin,ceftriaxone was 100%,to erythromycin,clindamycin over 50%,to other antimicrobial resistance in low resistance.Conclusion There were more SAU in children with respiratory infection; especially MRSA bears multi drug resistance.Therefore,the monitoring of drug resistance of Staphylococcus aureus shouldbe strengthened,and antimicrobial drugs should be rationally chosen accordingto the results of drug sensitive test for individuals,so that resistant strainscan be under effective control and kept being reduced from emergence.

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备注/Memo

备注/Memo:
作者简介:刘 青(1972-),男,大专,副主任技师,主要从事微生物检验工作,Tel:15920034339,E-mail:445232653@qq.com。
更新日期/Last Update: 2016-06-25