[1]李大鹏a,吕春雷b,王凤娇a,等.添加丙氨酰谷氨酰胺血液保存液对储存血液红细胞影响的实验研究[J].现代检验医学杂志,2016,31(06):108-111.[doi:10.3969/j.issn.1671-7414.2016.06.031]
　LI Da-penga,LV Chun-leib,WANG Feng-jiaoa,et al.Effect of Alanyl-Glutamine in Blood Preservation Solution on the Storage of Red Blood Cells[J].Journal of Modern Laboratory Medicine,2016,31(06):108-111.[doi:10.3969/j.issn.1671-7414.2016.06.031]
点击复制

添加丙氨酰谷氨酰胺血液保存液对储存血液红细胞影响的实验研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
108-111
栏目:
研究简报·实验技术
出版日期:
2016-12-20

文章信息/Info

Title:
Effect of Alanyl-Glutamine in Blood Preservation Solution on the Storage of Red Blood Cells
作者:
李大鹏a; 吕春雷b; 王凤娇a; 任红c; 郑文哲b
解放军88医院a.中心实验室; b.医务处; c.输血科,山东泰安 271000
Author(s):
LI Da-penga; LV Chun-leib; WANG Feng-jiaoa; REN Hongc; ZHENG Wen-zheb
a.Centrality Laboratory; b.Medical Service; c.Department of Blood Transfusion,the 88th Hospital of PLA,ShandongTaian 271000,China
关键词:
血液;  输血;  血液保存液;  抗凝剂;  丙氨酰谷氨酰胺;  游离血红蛋白
分类号:
R531.3; R381
DOI:
10.3969/j.issn.1671-7414.2016.06.031
文献标志码:
A
摘要:
目的 探讨添加丙氨酰谷氨酰胺的血液保存液对储存血液红细胞的影响。方法 选取无菌试管20只均分为试验组(A)和对照组(B),A组1～10管中分别加入丙氨酰谷氨酰胺稀释液0.756 ml及血液保存液Ⅲ0.42 ml,B组1～10管分别加入血液保存液Ⅲ0. 42 ml; 选取同性别无偿献血人员10例,采集未抗凝静脉血,分别对应加入A,B两组1～10试管中各3 ml,4～6℃冰箱保存; 采血后第10,21,35天分别检测A,B两组血浆游离血红蛋白(FHB)含量,并予以统计分析。结果 不同检测时间A组FHB分别为13.687±3.490,24.677±11.510,48.081±16.412mg/L,B组FHB分别为31.319±13.949,47.753±22.231,71.346±23.342 mg/L,各检测时间之间FHB含量差异均有统计学显著性意义(P<0.05); 相同检测时间A,B两组间FHB含量差异均有统计学显著性意义(P<0.05),A组均显著低于B组。结论储存血液随保存时间延长,血浆FHB含量显著升高,添加丙氨酰谷氨酰胺血液保存液Ⅲ对储存红细胞具有显著保护作用,对红细胞膜保护作用优于血液保存液Ⅲ,可显著降低红细胞溶血及血浆FHB含量,提高储存血液质量及输注效果。
Abstract:
Objective To study the influence of alanyl-glutamine in blood preservation solution on the storage of red blood cells.Methods Twenty sterile tubes were divided into two group:experimental group and control group(n=10).The 0.42 ml blood preservation solution III was added into each tube in both groups.Additionally,the 0.756 ml alanyl-glutamine solution was further added intoeach tube of experimental group.The 3 ml of venous blood from clinical donors was preserved in each tube of bothgroups at 4～6℃.The FHB(Free hemoglobin)in the plasma of each tube was detected after 10,21 and 35 days for analysis.Results Theamout of FHB in experimental group and control group were respectively(13.687±3.490 vs 31.319±13.949)mg/L,(24.677±11.510 vs 47.753±22.231)mg/Land(48.081±16.412 vs 71.346±23.342)mg/L respectively after 10,21 and35 days.The results between groups at each point were significantly different(P<0.05),which was lower in the expermental group than that of controlgroup(P<0.05).Conclusion The amout of FHB in theplasma of storage increased with time.The alanyl-glutamine in preservation solution Ⅲ was proven to have protective effect on the red blood cells of storage,and had protective effect excel blood preservation solution Ⅲ in erythrocyte membrane.The FHB and hemolysis were ameliorated and it thus improved the quality of blood storage and infusion effect.

参考文献/References:

[1] 张 永,黎红平,李慈梅.盐酸小檗碱对血浆游离血红蛋白的影响[J].现代医院,2012,12(8):77-78. Zhang Y,Li HP,Li CM.The affect of berberine hy-drochloride on plasma free hemoglobin[J].Modern Hospital,2012,12(8):77-78.
[2] 王九兵,王永维.红细胞悬液辐照前后在不同贮存期游离血红蛋白含量的比较[J].医学理论与实践,2012,25(14):1756-1758. Wang JB,Wang YW.The comparison of free hemoglobin content of different storage period for red blood cell irradiation[J].J Med Theor & Prac,2012,25(14):1756-1758.
[3] 袁志军.大量输血前后血清钾的变化和输血后高钾血症发生的影响因素[J].中国实用医药,2014,9(15):79-80. Yuan ZJ.Influence factors on the change of serum potassium for a large number of blood transfusion before and after and the hyperkalemia occurred after blood transfusion[J].China Piac Med,2014,9(15):79-80.
[4] 王 勇,欧阳孟林.洗涤式自体血回输中不同温度洗涤液对人红细胞形态结构的影响[J].中国现代医生,2014,52(14):31-33. Wang Y,Ouyang ML.Effect of different temperature scrubbing solution on red blood cell morphological structure[J].China Modern Doctor,2014,52(14):31-33.
[5] 冯 倩,刘 奕,王 彦,等.不同保存期内全血质量变化的研究[J].科学技术与工程,2013,13(13):3691-3694,3709. Feng Q,Liu Y,Wang Y,et al.Study on the quality changes of the whole blood during storage time[J].Science Technology and Engineering,2013,13(13):3691-3694,3709.
[6] 张 微,徐国美,刘青宁.不同配方洗涤液对浓缩血小板的洗涤效果研究[J].中国输血杂志,2010,23(11):944-946. Zhang W,Xu GM,Liu QN.Research on the washing effect for differentfor mulations of concentrated platelet washing liquid[J].Chinese Journal ofBlood Transfusion,2010,23(11):944-946.
[7] 陈云庆.血液保存液Ⅱ在高危出血患者血液透析封管中的应用体会[J].吉林医学,2011,32(32):6773-6774. Chen YQ.Application experience on the blood preservation solutionⅡ in patients at high risk of bleeding for the sealing tube of blood dialysis[J].Jilin Medical Journal,2011,32(32):6773-6774.
[8] 黄小辉,胡炜华.术前预存自体血液联合术中血液稀释在外科手术中的应用[J].南昌大学学报(医学版),2011,51(9):64-67. Huang XH,Hu WH.Combined application of predebosit autotransfusionand intraoperative hemodilution in surgical operation[J].Journal of NanchangUniversity(Medical Science),2011,51(9):64-67.
[9] 刘海波.游离血红蛋白指标在悬浮红细胞质量控制中的应用[J].国际检验医学杂志,2012,33(21):2639,2667. Liu HB.Application on the free hemoglobin target in quality controlof suspended red blood cells[J].Int J Lab Med,2012,33(21):2639,2667.
[10] 崔 莹,吴春雪,杨 龙,等.丙氨酰谷氨酰胺对急性肺损伤患者的保护作用及其机制初探[J].临床荟萃,2012,27(4):298-300,303. Cui Y,Wu CX,Yang L,et al.Studies on protection effect and mechanism of analyl-glutamine during acute lung injury[J].Clinical Focus,2012,27(4):298-300,303.
[11] 郭永军,孔媛媛,关永东,等.外源性补充谷氨酰胺对危重病患者的影响[J].中华全科医学,2012,10(1):49,97. Guo YJ,Kong YY,Guan YD,et al.Effects of ectrinsic glutamine on patients with critical illness[J].Chinese Journal of General Practice,2012,10(1):49,97.
[12] 魏 超,庄 远,汪德清.红细胞保存时间与功能变化的研究进展[J].中国输血杂志,2013,26(11):1152-1155. Wei C,Zhuang Y,Wang DQ.The research prgress on the save time and function change of red blood cells[J].Chin J Blood Transfusion,2013,26(11):1152-1155.

相似文献/References:

[1]戴芳,党 妮,李建武.AIHA患者应用两种配血方法的输血疗效比较[J].现代检验医学杂志,2016,31(02):130.[doi:10.3969/j.issn.1671-7414.2016.02.039]
　DAI Fang,DANG Ni,LI Jian-wu.Comparison on the Efficacy of Two Kinds of Blood Matching Method for Autoimmune Hemolytic Anemia[J].Journal of Modern Laboratory Medicine,2016,31(06):130.[doi:10.3969/j.issn.1671-7414.2016.02.039]
[2]党盼玉,王文婷,刘志新,等.Lewis血型系统抗体检出情况分析及其临床输血策略的探讨[J].现代检验医学杂志,2019,34(03):128.[doi:10.3969/j.issn.1671-7414.2019.03.032]
　DANG Pan-yu,WANG Wen-ting,LIU Zhi-xin,et al.Analysis of Lewis Blood Group System Antibody Detectionand Discussion on Clinical Transfusion Strategy[J].Journal of Modern Laboratory Medicine,2019,34(06):128.[doi:10.3969/j.issn.1671-7414.2019.03.032]

备注/Memo

备注/Memo:
基金项目:军队“十二五”后勤科研计划项目(课题编号:CWS11J297)。 作者简介:李大鹏(1964-),男,本科,副主任技师,研究方向:医学检验,Tel:13583848186,E-mail:lidapeng1964@126.com。 通讯作者:吕春雷,男,主任医师,Tel:15505486678,E-mail:15653808901@163.com。
更新日期/Last Update: 2016-12-20