[1]李腾达,刘鹏,谷明莉,等.sCD93在免疫性血小板减少症患者中表达升高及临床意义[J].现代检验医学杂志,2016,31(06):115-117.[doi:10.3969/j.issn.1671-7414.2016.06.033]
　LI Teng-da,LIU Peng,GU Ming-li,et al.Higher Expression of Soluble CD93 in Patients with Immune Thrombocytopenia and Clinical Significance[J].Journal of Modern Laboratory Medicine,2016,31(06):115-117.[doi:10.3969/j.issn.1671-7414.2016.06.033]
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sCD93在免疫性血小板减少症患者中表达升高及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
115-117
栏目:
研究简报·实验技术
出版日期:
2016-12-20

文章信息/Info

Title:
Higher Expression of Soluble CD93 in Patients with Immune Thrombocytopenia and Clinical Significance
作者:
李腾达1; 刘鹏1; 谷明莉1; 邓顺江1; 吴林洪1; 叶辛1; 刘云1; 张薇薇1; 钱琤2; 邓安梅1
1.第二军医大学长海医院,上海 200433; 2.解放军100医院,江苏苏州 215007
Author(s):
LI Teng-da1; LIU Peng1; GU Ming-li1; DENG Shun-jiang1; WU Lin-hong1;  YE Xin1; LIU Yun1; ZHANG Wei-wei1; QIAN Cheng2; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433,China; 2.No.100 Hospital of PLA,Jiangsu Suzhou 215007,China
关键词:
免疫性血小板减少症;  sCD93;  肿瘤坏死因子-α;  白介素-17
分类号:
R558.2; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.06.033
文献标志码:
A
摘要:
目的 探究sCD93在免疫性血小板减少症(ITP)患者中的表达水平及其与TNF-α等细胞因子的相关性,分析其临床意义。方法 收集2012年5月～2015年8月在上海长海医院血液科确诊的34例ITP患者和同期体检的34例健康个体外周血,分别作为实验组和对照组,以酶联免疫吸附法(ELISA)检测血浆中sCD93,TNF-α,IL-4,IL-17和IFN-γ的蛋白表达水平,对sCD93与TNF-α等细胞因子进行Pearson相关性分析。结果 实验组与对照组sCD93为(367.28±206.98 vs 218.43±96.14)ng/ml,差异具有统计学意义(t=3.803,P=0.000 3); sCD93与TNF-α,IL-17呈正相关,差异具有统计学意义(r=0.448,0.473,P均<0.01)。结论 sCD93可能参与了ITP疾病免疫机制,是该病潜在的疾病预测指标和研究靶点。
Abstract:
Objective To probe into the expression level of sCD93 in patients with Immune thrombocytopenia(ITP)and its correlation withcytokines such as TNF-α,and analyze its clinical significance.Methods Peripheral blood of 34 patients diagnosed as ITP in hematologydepartment and 34 health controls at the same time was collected from Changhai Hospital,in Shanghai during May,2012 to August,2015 and classified as experimental and controlled groups respectively.Enzyme-linked immunosorbent assay(ELISA)was used to test protein level of sCD93,TNF-α,IL-4,IL-17 and IFN-γin plasma,Pearson correlation analysis was to measure the relation between sCD93 and cytokines such as TNF-α.Results sCD93 in experimental and controlled columns was(367.28±206.98 vs 218.43±96.14 ng/ml,t=3.803,P=0.000 3)with statistical difference; sCD93 was positively related with TNF-α,IL-17(r=0.448, 0.473,P<0.01)and there were differences in statistics.Conclusion sCD93 might involve in immunemechanism of ITP,it was a potential predictor and studied target for this disease.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,12MA056,15ZD009,15XD007),美捷登基金(MJR20150019)。 作者简介:李腾达(1990-),女,在读硕士研究生,主要从事自身免疫研究,Tel: 18302192042,E-mail:tengdali@smmu.edu.cn。 刘 鹏(1982-),女,在读博士研究生,主要从事自身免疫研究,E-mail:ppliu23@163.com,共同第一作者。 通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。 钱 琤,女,检验技师,E-mail:qiancheng824@126.com,共同通讯作者。
更新日期/Last Update: 2016-12-20